Detailed information NTG0642294

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: LZ605_RS20305
• Genome accession: NZ_CP090423
• Coordinates: 4356397..4358124 (+)
• Length: 575 a.a.
• NCBI ID: WP_249843090.1
• Uniprot ID: -
• Organism: Stenotrophomonas maltophilia strain GYH
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4351397..4363124

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LZ605_RS20290 (LZ605_20290)	-	4351791..4352228 (+)	438	WP_249843087.1	pilin	-
LZ605_RS20295 (LZ605_20295)	-	4352320..4353195 (+)	876	WP_249843088.1	sterol desaturase family protein	-
LZ605_RS20300 (LZ605_20300)	-	4353327..4356383 (+)	3057	WP_249843089.1	fused MFS/spermidine synthase	-
LZ605_RS20305 (LZ605_20305)	pilB	4356397..4358124 (+)	1728	WP_249843090.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LZ605_RS20310 (LZ605_20310)	pilR	4358207..4359580 (-)	1374	WP_249843091.1	sigma-54 dependent transcriptional regulator	Regulator
LZ605_RS20315 (LZ605_20315)	-	4359641..4361254 (-)	1614	WP_249843092.1	ATP-binding protein	-
LZ605_RS20320 (LZ605_20320)	sucC	4361637..4362806 (+)	1170	WP_004154430.1	ADP-forming succinate--CoA ligase subunit beta	-

Sequence

Protein

• Length: 575 a.a.
• Molecular weight: 62328.34 Da
• Isoelectric Point: 5.8733

>NTDB_id=642294 LZ605_RS20305 WP_249843090.1 4356397..4358124(+) (pilB) [Stenotrophomonas maltophilia strain GYH]
MSVVSPPNLVGITGLARRLVQDGALDEATARDAMAKATAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDAS
QNAMNLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVETILVDEDQIRRTLEQWHASHDTLGDTLGGD
DEGMANLDVGLGDEDNAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFM
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPNCKRPAQLPEHA
LLAEGFSQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide

• Length: 1728 bp

>NTDB_id=642294 LZ605_RS20305 WP_249843090.1 4356397..4358124(+) (pilB) [Stenotrophomonas maltophilia strain GYH]
ATGAGCGTTGTTTCCCCCCCCAATCTGGTCGGCATCACCGGCCTGGCCCGGCGCCTGGTCCAGGACGGTGCCCTGGACGA
GGCCACCGCGCGCGATGCCATGGCCAAGGCCACGGCGGCCCGCCAGCCACTGCCCACCTGGTTCGCGCAGAACAAGCTGG
TCAGCGCCTCGCAGCTGGCGGCGGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGATGCCAGC
CAGAACGCGATGAACCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAACCCGACCCACGCGCTGGATGAGATCAAGTTCCACACCAATCTGGTGGTGGAGACCATCCTGG
TCGACGAGGATCAGATCCGCCGCACCCTGGAGCAGTGGCACGCCAGCCATGACACGCTGGGCGACACCCTCGGCGGCGAC
GACGAGGGGATGGCCAACCTCGATGTCGGTCTCGGCGACGAAGACAATGCCGGTGGCGACTCCGGCATCGACGCCAAGGG
CGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCCATCCGCAAGGGCGCCTCGGACATCCACTTCG
AGCCGTACGAAGACGACTATCGCGTGCGCCTGCGCATCGATGGCCTGTTGAAGATGGTCGCGCGTGCGCCGGTGAAGCTG
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGACGGGCGCAT
CAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGTGAGAAGGTGGTGC
TGCGTATCCTGGATGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCATG
GATGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCCGCACTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCCATCAAGGCAGCGCAGACCGGTCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGACTGATGAACATGGGCATTGCGCCGTACAACATCACCAGTTCGG
TGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGTCTGTGCCCGAACTGCAAGCGCCCGGCGCAGTTGCCCGAACACGCG
CTGTTGGCCGAAGGCTTCAGCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTATGAGGCGGTGGGCTGCGATGAATGCAC
CGAAGGCTACAAGGGGCGTACCGGCGTCTACCAGGTGATGCCGATGACCGATGAGATCGCAACCATTGTGCTGGCCGGCG
GTAATGCACTGCAGATTGCCGAGGCGGCGCAGCAGATTGGAGTGAATGATCTGCGCCAGTCGGCGTTGAAGAAGGCCGCG
GCCGGCGTCACCAGCCTGGCCGAGATCAACCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-150)

• Type II/IV secretion system protein (T2SSE) (198-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.614	98.609	0.558
pilB	Acinetobacter baylyi ADP1	55.459	100	0.557
pilB	Legionella pneumophila strain ERS1305867	51.489	99.304	0.511
pilF	Neisseria gonorrhoeae MS11	48.854	98.609	0.482
pilB	Vibrio cholerae strain A1552	48.763	98.435	0.48
pilB	Vibrio parahaemolyticus RIMD 2210633	46.195	98.261	0.454
pilB	Vibrio campbellii strain DS40M4	44.991	98.957	0.445
pilB/pilB1	Synechocystis sp. PCC 6803	36.007	100	0.383
pilF	Thermus thermophilus HB27	37.746	97.217	0.367
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.095	94.957	0.362