Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LXF01_RS01285 Genome accession   NZ_CP090067
Coordinates   275012..276721 (-) Length   569 a.a.
NCBI ID   WP_233944932.1    Uniprot ID   -
Organism   Acinetobacter sp. SH20PTE14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 270012..281721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXF01_RS01255 (LXF01_01255) - 270077..270400 (+) 324 WP_067763086.1 pyrimidine/purine nucleoside phosphorylase -
  LXF01_RS01260 (LXF01_01260) rlmB 270578..271336 (+) 759 WP_233944930.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LXF01_RS01265 (LXF01_01265) - 271342..272262 (+) 921 WP_180156708.1 DMT family transporter -
  LXF01_RS01270 (LXF01_01270) coaE 272281..272883 (-) 603 WP_233944931.1 dephospho-CoA kinase -
  LXF01_RS01275 (LXF01_01275) pilD 272893..273753 (-) 861 WP_180156713.1 A24 family peptidase Machinery gene
  LXF01_RS01280 (LXF01_01280) pilC 273753..274979 (-) 1227 WP_078192563.1 type II secretion system F family protein Machinery gene
  LXF01_RS01285 (LXF01_01285) pilB 275012..276721 (-) 1710 WP_233944932.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LXF01_RS01290 (LXF01_01290) tpiA 276997..277788 (+) 792 WP_180084538.1 triose-phosphate isomerase -
  LXF01_RS01295 (LXF01_01295) secG 277802..278131 (+) 330 WP_180084539.1 preprotein translocase subunit SecG -
  LXF01_RS01325 (LXF01_01325) rimP 279062..279586 (+) 525 WP_078192559.1 ribosome maturation factor RimP -
  LXF01_RS01330 (LXF01_01330) nusA 279622..281106 (+) 1485 WP_078192558.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63668.66 Da        Isoelectric Point: 4.8371

>NTDB_id=640529 LXF01_RS01285 WP_233944932.1 275012..276721(-) (pilB) [Acinetobacter sp. SH20PTE14]
MTAVQGSSRFTGFIRRLVDEGHLSAEKMQTALQNSKKAHQDIVPHLIQEYHLSARLIAETISIEFGEPVFDISTYDTSLI
IRDEFEDKLITKHRILPIFKHGNLLFVAISNPTNIEAVDAIRFGSKLNTETIIVEHDKLEKLIEQNFAEESTFDFDDDFD
LDLEQENIDPAKEDEDEATGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKTYRVRYRVDGVLRTITNPPLQLATRLASR
LKVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMQALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKETVDVPKQSLLELGFT
EDDLNTPEFQIYQPVGCAECREGYKGRVGIYEVMKVTPEISRIIMEDGNALQIAEASNQAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=640529 LXF01_RS01285 WP_233944932.1 275012..276721(-) (pilB) [Acinetobacter sp. SH20PTE14]
ATGACAGCAGTTCAAGGTTCTTCAAGATTTACGGGGTTTATTCGTCGCTTAGTTGACGAAGGGCATCTGTCTGCCGAAAA
AATGCAAACAGCGCTGCAAAATTCAAAAAAGGCTCATCAAGATATCGTGCCGCATTTGATTCAGGAATATCACCTTTCAG
CACGCCTGATCGCAGAAACCATCTCGATAGAGTTCGGTGAACCGGTTTTTGATATTTCAACTTATGATACGTCTTTAATT
ATACGTGATGAATTTGAAGATAAACTCATTACCAAGCATCGTATTTTGCCTATTTTTAAACATGGCAATTTATTATTCGT
TGCGATCAGTAATCCCACCAATATTGAAGCCGTAGATGCCATCCGATTTGGTAGTAAGCTCAATACCGAAACCATTATTG
TTGAACATGACAAACTTGAAAAGTTAATTGAACAGAACTTTGCTGAAGAAAGTACTTTTGACTTTGATGATGACTTTGAT
CTTGATTTAGAACAGGAAAATATTGATCCAGCGAAAGAAGATGAAGATGAGGCAACAGGGGATGAAGCTCCGATTGTTAA
ATACATCAATAAACTACTGGTCGACGCCATTCGTATGGGTGCATCTGACTTACATTTTGAACCCTACGAAAAAACCTATC
GCGTCCGCTACCGTGTCGATGGCGTGTTAAGAACAATTACAAATCCGCCATTACAGCTCGCTACACGACTCGCTTCACGT
TTAAAAGTCATGTCACAAATGGACATCTCGGAAAAGCGTATACCTCAAGACGGTCGTATCAAGTTAAAGCTGTCAAAGAA
CAAAGCTATCGACTTCCGTGTGAACTCTTTACCGACATTGTTTGGTGAAAAACTTGTTCTACGTATCCTTGATCCATCTA
GTGCGATGCTAGGGATTGATGCGTTGGGTTATGAACCGGATCAAAAAGAACTGTTCATGCAGGCTTTAGAAAAACCACAA
GGCATGCTACTCATTACCGGTCCAACGGGTTCCGGTAAAACCGTATCTCTATATACAGGTTTAAATATTCTCAACCGTGA
AGATACCAATATTTCAACGGCCGAAGACCCTGTGGAAATTAACTTAGAAGGGATCAATCAGGTCAACGTAAATAATAAAG
TCGGCCTCACCTTCTCTGCAGCCTTAAAATCATTCTTACGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGAGAT
TTAGAAACGGCTGAAATTGCAATTAAAGCGGCTCAAACAGGTCACATGGTGATGTCTACACTGCATACCAATAGTGCACC
TGAAACACTGACACGCTTACGCAATATGGGCGTGCCTTCATTTAACATCGCGACATCCGTGAACCTTGTCATTGCGCAGC
GACTTGCACGTCGCCTATGTTCACAATGTAAAGAAACAGTAGACGTACCAAAACAAAGTCTATTGGAGCTTGGTTTTACC
GAAGATGATTTAAATACACCAGAATTCCAAATATACCAGCCTGTTGGTTGTGCCGAATGCCGTGAAGGCTATAAAGGTCG
TGTGGGTATTTACGAAGTCATGAAAGTGACACCTGAAATTTCAAGAATTATTATGGAAGACGGCAATGCCTTACAAATTG
CCGAAGCTTCAAATCAAGCAGGTTTCAACAATTTACGCCGTTCAGGCTTAATTAAGGTCATGCAGGGGGTGACTTCACTT
CAAGAAGTCAATCGTGTGACCAGTGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.454

100

0.81
  pilB Acinetobacter baylyi ADP1

74.912

100

0.75
  pilB Legionella pneumophila strain ERS1305867

54.034

93.673

0.506
  pilF Neisseria gonorrhoeae MS11

48.932

98.77

0.483
  pilB Vibrio cholerae strain A1552

50.469

93.673

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

47.217

97.891

0.462
  pilB Vibrio campbellii strain DS40M4

46.679

97.891

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

34.244

100

0.374