Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LU301_RS08165 Genome accession   NZ_CP089978
Coordinates   1645495..1647216 (+) Length   573 a.a.
NCBI ID   WP_305269636.1    Uniprot ID   -
Organism   Moraxella sp. ZY210820     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1640495..1652216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU301_RS08130 (LU301_08115) ttcA 1640517..1641422 (+) 906 WP_305269624.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  LU301_RS08135 (LU301_08120) - 1641434..1641817 (+) 384 WP_305269627.1 SCP2 sterol-binding domain-containing protein -
  LU301_RS08140 (LU301_08125) - 1642029..1642604 (+) 576 WP_305269628.1 porin family protein -
  LU301_RS08145 (LU301_08130) - 1642737..1643291 (+) 555 WP_305269631.1 porin family protein -
  LU301_RS08150 (LU301_08135) pyrC 1643431..1644465 (+) 1035 WP_305269633.1 dihydroorotase -
  LU301_RS08155 (LU301_08140) rnt 1644495..1645157 (+) 663 WP_305274029.1 ribonuclease T -
  LU301_RS08165 (LU301_08150) pilB 1645495..1647216 (+) 1722 WP_305269636.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LU301_RS08170 (LU301_08155) pilC 1647238..1648464 (+) 1227 WP_305269638.1 type II secretion system F family protein Machinery gene
  LU301_RS08175 (LU301_08160) pilD 1648464..1649336 (+) 873 WP_305269640.1 A24 family peptidase Machinery gene
  LU301_RS08180 (LU301_08165) coaE 1649333..1649944 (+) 612 WP_305269642.1 dephospho-CoA kinase -
  LU301_RS08185 (LU301_08170) - 1649958..1650923 (-) 966 WP_305269645.1 YheT family hydrolase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63614.93 Da        Isoelectric Point: 4.7914

>NTDB_id=639629 LU301_RS08165 WP_305269636.1 1645495..1647216(+) (pilB) [Moraxella sp. ZY210820]
MSAQGNFKFTGFLRRLVEEELITPENMQSSIEASRKAQTDIVPYLIENHRIKPIQIAQAISNDFGEPLFDISLYNTSVVP
TGIVEEKVITKYKILPLLQRGNILYVATSNPTNIEAFDAIRFSSKLNVESIIVEHDKLEKVIGQLYNAEDNFTFSDDDMD
LDVEASSSEEEQDDDSGDEDSAPIVKYINKLLVDAIRMGASDLHFEPYEKVYRVRYRIDGVLRQIANPPLQLAGRLAARL
KVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKALFLEALEKPQG
MLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLQGINQVNVNPKVGLTFAAALKSFLRQDPDVVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKEMPKADVENMRPSLLE
LGFKAEELDNPEFKLFEPVGCSECREGFKGRVGVYEVMKVTPEISKIIMRDGNAIEIAEASEAAGFNNLRRSGLLKVMQG
VTTLQEISRVTAE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=639629 LU301_RS08165 WP_305269636.1 1645495..1647216(+) (pilB) [Moraxella sp. ZY210820]
ATGTCTGCTCAAGGAAATTTTAAATTTACAGGCTTTCTTCGACGTCTTGTTGAAGAAGAGCTTATTACGCCTGAAAATAT
GCAAAGTTCAATTGAAGCTTCTCGTAAAGCACAAACGGACATTGTACCATACTTAATAGAAAATCATCGTATTAAACCTA
TTCAAATTGCACAAGCGATTTCTAATGATTTTGGTGAGCCATTATTTGATATTTCTTTATATAATACTTCTGTTGTTCCT
ACAGGTATTGTAGAAGAAAAAGTCATTACAAAATACAAAATATTACCTCTATTACAACGTGGTAATATCTTATATGTTGC
AACCAGTAACCCAACTAATATTGAAGCTTTTGATGCAATTCGCTTTAGTAGTAAGCTCAATGTCGAATCAATTATTGTCG
AACATGATAAATTAGAAAAAGTAATTGGTCAGCTTTATAATGCTGAAGATAATTTTACTTTTTCTGATGATGATATGGAT
CTTGATGTAGAAGCTAGTAGTTCAGAAGAAGAGCAAGATGATGATAGTGGAGATGAGGATTCAGCACCTATTGTAAAATA
TATTAATAAACTCCTAGTCGATGCGATTCGTATGGGTGCTTCTGACTTACACTTTGAACCTTATGAAAAAGTATATCGTG
TACGTTATCGTATAGATGGTGTATTAAGACAAATTGCTAATCCTCCATTACAATTAGCAGGACGTTTAGCTGCTCGTCTA
AAAGTTATGTCACAAATGGACATTTCTGAAAAACGTATTCCACAAGATGGACGTATTAAGCTTAAATTATCAAAAACCAA
AGCAATTGATTTTCGTGTAAACTCATTACCAACCTTATTTGGTGAGAAATTAGTACTACGTATCCTTGACCCATCAAGTG
CCATGTTAGGTATTGATGCACTTGGTTATGAACCTGATCAAAAAGCACTATTTTTAGAAGCACTTGAAAAACCTCAAGGT
ATGTTATTAATTACAGGTCCTACAGGTTCTGGTAAAACTGTTTCTCTCTATACTGGTTTAAATATTTTAAATACTGAAGA
TACTAATATATCAACAGCTGAAGATCCTGTGGAAATCAACTTACAAGGCATTAACCAAGTAAACGTTAATCCTAAAGTTG
GCTTAACATTCGCTGCAGCACTCAAATCATTCTTACGTCAAGACCCTGATGTAGTCATGGTCGGTGAGATTCGTGATTTA
GAGACTGCTGAAATTGCAATTAAAGCTGCACAAACAGGACACATGGTAATGTCCACCCTACACACCAACAGTGCTCCTGA
AACCTTGACACGTCTACGTAATATGGGTGTACCATCATTTAACATTGCCACTTCAGTAAACTTGGTGATTGCACAGCGAC
TTGCACGCCGACTCTGTTCTCAATGTAAAAAAGAAATGCCTAAAGCTGATGTTGAAAATATGCGCCCTAGTTTACTCGAA
CTTGGTTTTAAAGCTGAAGAGTTAGATAATCCAGAATTTAAACTATTTGAACCAGTGGGATGCTCTGAATGTCGTGAAGG
CTTTAAAGGACGTGTTGGTGTGTATGAAGTGATGAAAGTGACTCCTGAAATTTCCAAAATTATTATGCGTGATGGTAATG
CAATTGAAATTGCAGAAGCTTCTGAAGCTGCTGGCTTTAATAATTTACGCCGTTCTGGTTTACTTAAAGTCATGCAAGGT
GTTACAACCTTACAAGAAATTAGCCGTGTAACTGCTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

76.45

99.302

0.759
  pilB Acinetobacter baylyi ADP1

73.111

99.302

0.726
  pilB Legionella pneumophila strain ERS1305867

51.562

100

0.518
  pilF Neisseria gonorrhoeae MS11

50.179

97.731

0.49
  pilB Vibrio cholerae strain A1552

48.866

100

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.135

98.255

0.473
  pilB Vibrio campbellii strain DS40M4

47.038

97.208

0.457
  pilF Thermus thermophilus HB27

37.057

98.429

0.365