Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LU301_RS03640 Genome accession   NZ_CP089978
Coordinates   715972..717168 (-) Length   398 a.a.
NCBI ID   WP_305272629.1    Uniprot ID   -
Organism   Moraxella sp. ZY210820     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 710972..722168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU301_RS03600 (LU301_03590) - 711167..711826 (+) 660 WP_305272613.1 NAD(P)H-dependent oxidoreductase -
  LU301_RS03605 (LU301_03595) - 711944..712426 (+) 483 WP_305272615.1 META domain-containing protein -
  LU301_RS03610 (LU301_03600) mpl 712479..713846 (-) 1368 WP_305272619.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -
  LU301_RS03615 (LU301_03605) - 714185..714760 (-) 576 WP_370692237.1 IS630 family transposase -
  LU301_RS03620 (LU301_03610) - 714744..715064 (-) 321 WP_305272622.1 IS630 transposase-related protein -
  LU301_RS03630 (LU301_03620) - 715456..715725 (-) 270 WP_305272625.1 hypothetical protein -
  LU301_RS03635 (LU301_03625) - 715694..715912 (-) 219 WP_305272627.1 hypothetical protein -
  LU301_RS03640 (LU301_03630) pilU 715972..717168 (-) 1197 WP_305272629.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LU301_RS03650 (LU301_03640) - 717570..718877 (-) 1308 WP_305272632.1 L,D-transpeptidase -
  LU301_RS03655 (LU301_03645) rplI 719018..719464 (-) 447 WP_305272636.1 50S ribosomal protein L9 -
  LU301_RS03660 (LU301_03650) rpsR 719474..719701 (-) 228 WP_305272639.1 30S ribosomal protein S18 -
  LU301_RS03665 (LU301_03655) rpsF 719716..720105 (-) 390 WP_305272642.1 30S ribosomal protein S6 -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 44788.21 Da        Isoelectric Point: 6.2801

>NTDB_id=639623 LU301_RS03640 WP_305272629.1 715972..717168(-) (pilU) [Moraxella sp. ZY210820]
MSTEESSPLFTEETLAEARRIMFNMLTKVVEVGGSDLFITAEFPPSIKHQGSMKPLSKQALTADKTKLFAYSLMNERQRA
EFEEQLECNFAINVPEVSRFRVNIFVQQLQVGMVIRTITAEIPNFAKLRLPEKLKDVIMEKRGLVLVVGGTGSGKSTSLA
AMIDHRNENSAGHIITVEDPVEYVHKHKKSMITHREVGVDTHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETG
HLCLGTLHANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIVSQRLIRTQDGQGRRAAVEIMLNTPLISDLILKGQMHE
LKEIMGKSRELGMQTFDQALFDLYDEGAISYEEAIRNADSANELRLQIKLKSKRGGEQSSPSVGLSLHKDEEEKTEGE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=639623 LU301_RS03640 WP_305272629.1 715972..717168(-) (pilU) [Moraxella sp. ZY210820]
ATGAGTACCGAAGAATCATCTCCATTATTTACTGAAGAAACACTTGCTGAAGCACGTCGTATTATGTTTAATATGCTCAC
TAAAGTTGTTGAAGTGGGTGGTTCTGATTTATTTATTACTGCTGAATTTCCACCAAGTATTAAACATCAAGGTTCGATGA
AACCTTTAAGTAAACAAGCACTTACAGCAGATAAAACTAAACTTTTTGCTTATAGCTTAATGAATGAACGACAACGTGCA
GAATTTGAAGAACAATTAGAATGTAATTTTGCAATTAATGTCCCTGAAGTTTCACGTTTCCGCGTTAATATTTTTGTACA
ACAATTACAAGTTGGTATGGTCATCCGTACGATTACTGCAGAGATTCCAAACTTTGCAAAATTGCGTTTACCTGAGAAAC
TCAAAGATGTGATTATGGAAAAACGTGGTCTTGTACTTGTCGTAGGTGGTACTGGTTCTGGTAAATCTACATCATTGGCT
GCCATGATTGATCATCGTAATGAAAATTCAGCAGGTCATATTATTACTGTTGAAGACCCTGTTGAATATGTTCACAAACA
CAAAAAATCAATGATTACCCATCGTGAGGTCGGTGTTGATACTCATACTTGGCATCACGCACTCAAAAATACGCTCCGCC
AAGCACCCGATGTAATTTTGATTGGGGAAATTCGTGATACTGAAACGATGGAACATGCGATTGCCTTTGCTGAAACAGGA
CATTTATGTTTAGGTACATTACACGCTAATAATGCAAACCAAACACTTGACCGTATTATTAACTTCTTCCCTGAAGAACG
TCGTAATCAATTATTGATGGATTTATCATCAAATATGAAAGCAATTGTATCACAGCGATTAATTCGTACACAAGATGGTC
AAGGTCGCCGTGCAGCTGTTGAAATCATGCTCAATACCCCATTAATTTCGGACTTAATTTTAAAAGGACAAATGCATGAA
CTAAAAGAAATCATGGGTAAATCTCGTGAATTAGGTATGCAAACCTTTGACCAAGCTTTATTTGATTTGTACGATGAAGG
TGCAATTTCTTATGAAGAAGCAATCCGTAATGCAGACTCTGCAAACGAACTTCGTTTACAAATTAAACTAAAGAGTAAAC
GTGGCGGTGAACAGAGTTCGCCATCAGTCGGTTTATCTTTACATAAAGATGAAGAAGAAAAAACTGAAGGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(97-290)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.378

92.965

0.543
  pilU Acinetobacter baylyi ADP1

57.471

87.437

0.503
  pilU Vibrio cholerae strain A1552

52

87.94

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.551

90.704

0.377
  pilT Acinetobacter nosocomialis M2

43.636

82.915

0.362
  pilT Acinetobacter baumannii D1279779

43.636

82.915

0.362
  pilT Acinetobacter baumannii strain A118

43.636

82.915

0.362