Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LVE68_RS15370 Genome accession   NZ_CP089928
Coordinates   3551275..3552987 (-) Length   570 a.a.
NCBI ID   WP_232941319.1    Uniprot ID   -
Organism   Microbulbifer sp. YPW16     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3546275..3557987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVE68_RS15345 (LVE68_15345) mutT 3547450..3547860 (+) 411 WP_232941314.1 8-oxo-dGTP diphosphatase MutT -
  LVE68_RS15350 (LVE68_15350) yacG 3548080..3548301 (-) 222 WP_232941315.1 DNA gyrase inhibitor YacG -
  LVE68_RS15355 (LVE68_15355) coaE 3548308..3548913 (-) 606 WP_232941316.1 dephospho-CoA kinase -
  LVE68_RS15360 (LVE68_15360) - 3549033..3549917 (-) 885 WP_232941317.1 A24 family peptidase -
  LVE68_RS15365 (LVE68_15365) pilC 3549930..3551141 (-) 1212 WP_232941318.1 type II secretion system F family protein Machinery gene
  LVE68_RS15370 (LVE68_15370) pilB 3551275..3552987 (-) 1713 WP_232941319.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVE68_RS15380 (LVE68_15380) pilE 3553525..3553968 (+) 444 WP_305080387.1 pilin Machinery gene
  LVE68_RS15385 (LVE68_15385) - 3554041..3555960 (+) 1920 WP_232941320.1 hypothetical protein -
  LVE68_RS15390 (LVE68_15390) - 3555960..3556823 (+) 864 WP_232941321.1 glycosyltransferase family 2 protein -
  LVE68_RS15395 (LVE68_15395) - 3556862..3557905 (+) 1044 WP_255701185.1 UDP-N-acetylglucosamine 2-epimerase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62359.21 Da        Isoelectric Point: 5.9234

>NTDB_id=639191 LVE68_RS15370 WP_232941319.1 3551275..3552987(-) (pilB) [Microbulbifer sp. YPW16]
MSSIPLSGLAKRLVADKKLDEATAASATKAAQRENQTFAQHAVEAKLINSRELASTAAHAFGSPLFDLGAYNFELIPQGV
VDDRLISKHFALPLYKRGNRLFVAVADPTNLSALDEINFNTGLNTDAVLVEADKLAKAIDSYLSHSGDMGAGLDGMDDEE
LDALDVEGSNKDQGQDDDPGGDEAPVVRFVNKVLLDAIRSGASDIHFEPYEKSYRVRFRTDGVLHEIANPPIQLQARISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKMGIDALGYEEDQKKMYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSSKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSLIIAQRLARRLCSECKKPVTLPAEVLKEEGF
DMVSIPQKEWKIYQPVGCEHCSKGYKGRVGVYEVVRITQGISRIIMEGGNSIQIADQARKEGFNNLRNSALRKVVMGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=639191 LVE68_RS15370 WP_232941319.1 3551275..3552987(-) (pilB) [Microbulbifer sp. YPW16]
ATGAGTAGTATTCCCCTCAGCGGCCTGGCCAAGCGACTGGTAGCCGACAAAAAACTGGACGAAGCCACCGCGGCCTCTGC
CACAAAGGCAGCCCAGCGCGAGAACCAGACTTTTGCCCAGCATGCAGTAGAGGCCAAACTCATCAACAGTCGTGAGCTGG
CCAGCACCGCCGCTCATGCATTCGGCTCACCTCTGTTTGACCTGGGTGCCTACAATTTCGAGCTGATCCCGCAGGGTGTG
GTTGACGACAGGCTAATAAGCAAGCACTTCGCCCTGCCCCTCTATAAGCGTGGCAATCGGCTGTTTGTCGCTGTCGCAGA
CCCCACCAACCTGTCCGCGCTGGACGAAATCAATTTCAACACCGGCCTGAACACTGATGCCGTCCTGGTAGAAGCCGACA
AGCTTGCCAAAGCTATCGATAGCTACCTGTCCCATAGCGGGGACATGGGAGCAGGTCTCGATGGCATGGACGATGAGGAA
CTGGACGCTCTCGATGTAGAAGGAAGTAACAAAGACCAGGGCCAGGATGACGATCCCGGCGGTGACGAAGCCCCGGTTGT
CCGCTTTGTGAACAAGGTTCTACTGGATGCGATCAGGTCGGGCGCATCGGATATCCACTTCGAGCCGTATGAGAAGTCCT
ATCGGGTACGCTTCCGCACCGATGGCGTGCTGCACGAAATCGCCAACCCCCCAATTCAGCTTCAGGCTCGGATTTCAGCA
CGTTTGAAAGTCATGTCCAAGATGGACATCTCCGAACGCCGGGTTCCCCAGGACGGTCGCATCAAGATGAAGCTGTCCAA
GACCAAGGCGATTGACTTCCGGGTCAACAGTTTGCCTACATTATGGGGCGAGAAGATTGTACTCCGGATTCTGGATCCGT
CCTCAGCCAAAATGGGCATCGATGCCCTGGGGTATGAAGAAGACCAGAAGAAAATGTACATGGATGCCCTGGCCCAGCCA
CAGGGTATGATCCTCGTGACCGGCCCGACAGGTTCGGGTAAAACCGTCTCGCTTTACACCGGCCTGAACATCCTCAATAC
GCCTGAACGCAACATATCAACCGCGGAGGACCCGGTAGAGATTAATCTGGAGGGGATCAACCAGGTTAACGTTTCCAGTA
AGGTCGGGCTTAATTTCGCTGAGGCCCTCCGTTCCTTCTTGCGGCAGGACCCCGATATTGTGATGGTAGGTGAGATCCGG
GACCTGGAAACGGCAGAGATCGCAATCAAGGCTGCCCAAACGGGCCATCTGGTGCTATCCACCCTTCACACCAATTCCGC
CCCCGAAACCCTGACCCGCCTGATGAATATGGGCGTGCCTACTTTCAATATCGCCACCTCTGTAAGCCTGATCATTGCCC
AGCGGCTCGCACGTCGCCTCTGTAGCGAGTGCAAGAAGCCAGTCACCCTTCCAGCAGAGGTACTGAAAGAGGAGGGCTTC
GACATGGTCAGCATCCCACAAAAAGAGTGGAAGATCTATCAGCCGGTGGGCTGTGAGCACTGCTCGAAGGGCTATAAAGG
CCGGGTGGGTGTCTATGAAGTGGTTCGCATCACCCAAGGCATATCCCGAATTATCATGGAAGGTGGTAATTCCATCCAGA
TTGCCGACCAGGCTCGAAAAGAAGGGTTTAATAACCTTCGGAATTCGGCCCTGCGTAAGGTGGTAATGGGTGTGACAAGC
CTTGAAGAGGCAAACCGCGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.053

100

0.611
  pilB Acinetobacter baylyi ADP1

59.612

99.474

0.593
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.547
  pilB Vibrio parahaemolyticus RIMD 2210633

54.545

92.632

0.505
  pilB Vibrio campbellii strain DS40M4

53.686

92.807

0.498
  pilB Vibrio cholerae strain A1552

53.7

92.456

0.496
  pilF Neisseria gonorrhoeae MS11

50.854

92.456

0.47
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.817

94.912

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

41.6

87.719

0.365