Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LVE68_RS15380 Genome accession   NZ_CP089928
Coordinates   3553525..3553968 (+) Length   147 a.a.
NCBI ID   WP_305080387.1    Uniprot ID   -
Organism   Microbulbifer sp. YPW16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3548525..3558968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVE68_RS15360 (LVE68_15360) - 3549033..3549917 (-) 885 WP_232941317.1 A24 family peptidase -
  LVE68_RS15365 (LVE68_15365) pilC 3549930..3551141 (-) 1212 WP_232941318.1 type II secretion system F family protein Machinery gene
  LVE68_RS15370 (LVE68_15370) pilB 3551275..3552987 (-) 1713 WP_232941319.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVE68_RS15380 (LVE68_15380) pilE 3553525..3553968 (+) 444 WP_305080387.1 pilin Machinery gene
  LVE68_RS15385 (LVE68_15385) - 3554041..3555960 (+) 1920 WP_232941320.1 hypothetical protein -
  LVE68_RS15390 (LVE68_15390) - 3555960..3556823 (+) 864 WP_232941321.1 glycosyltransferase family 2 protein -
  LVE68_RS15395 (LVE68_15395) - 3556862..3557905 (+) 1044 WP_255701185.1 UDP-N-acetylglucosamine 2-epimerase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15135.13 Da        Isoelectric Point: 3.8453

>NTDB_id=639192 LVE68_RS15380 WP_305080387.1 3553525..3553968(+) (pilE) [Microbulbifer sp. YPW16]
MKQQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAQVTEAVNLMGGLKSTVTEIYSTEGSFDDADSGSLGIPAAADVT
GDNVQGVTVTNGVITAQMASSNVAEAIQDATISMSPVTSSGSIDWVCVPGLTGTALDNKYLPSECRT

Nucleotide


Download         Length: 444 bp        

>NTDB_id=639192 LVE68_RS15380 WP_305080387.1 3553525..3553968(+) (pilE) [Microbulbifer sp. YPW16]
ATGAAACAACAAAAGGGTTTTACTCTTATTGAATTGATGATCGTGGTAGCGATCATCGGTATTCTCGCTGCAGTAGCGCT
GCCGGCCTACCAGGACTACACCGCGCGTGCCCAGGTGACTGAGGCTGTTAACCTTATGGGTGGACTGAAGAGCACCGTGA
CTGAGATCTATAGTACTGAAGGTTCATTTGATGATGCTGACAGCGGATCACTTGGAATTCCTGCTGCCGCAGACGTCACC
GGCGATAACGTACAGGGCGTCACTGTTACTAATGGTGTAATCACTGCGCAGATGGCTTCCTCTAATGTTGCTGAGGCCAT
TCAGGACGCAACAATTTCCATGAGCCCGGTAACTAGTTCTGGTTCCATTGATTGGGTCTGCGTGCCGGGTCTTACAGGCA
CTGCCCTTGACAATAAGTACCTGCCGTCTGAGTGTCGCACCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

52.258

100

0.551
  pilE Neisseria gonorrhoeae MS11

47.742

100

0.503
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.87

100

0.449
  comP Acinetobacter baylyi ADP1

40.881

100

0.442
  pilA2 Legionella pneumophila strain ERS1305867

44.521

99.32

0.442
  pilA/pilA1 Eikenella corrodens VA1

43.046

100

0.442
  pilA Ralstonia pseudosolanacearum GMI1000

39.752

100

0.435
  pilA2 Legionella pneumophila str. Paris

43.836

99.32

0.435
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.838

100

0.381