Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LTQ02_RS10970 Genome accession   NZ_CP089205
Coordinates   2466421..2468106 (+) Length   561 a.a.
NCBI ID   WP_243578788.1    Uniprot ID   -
Organism   Vibrio splendidus strain 4_C08b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2461421..2473106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ02_RS10950 (LTQ02_10950) pdhR 2462922..2463683 (-) 762 WP_004735446.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  LTQ02_RS10955 (LTQ02_10955) ampD 2464164..2464763 (-) 600 WP_054541864.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LTQ02_RS10960 (LTQ02_10960) nadC 2464847..2465737 (+) 891 WP_017079961.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LTQ02_RS10965 (LTQ02_10965) pilA 2465993..2466421 (+) 429 WP_243578789.1 pilin Machinery gene
  LTQ02_RS10970 (LTQ02_10970) pilB 2466421..2468106 (+) 1686 WP_243578788.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LTQ02_RS10975 (LTQ02_10975) pilC 2468121..2469350 (+) 1230 WP_243578787.1 type II secretion system F family protein Machinery gene
  LTQ02_RS10980 (LTQ02_10980) pilD 2469418..2470287 (+) 870 WP_004735439.1 A24 family peptidase Machinery gene
  LTQ02_RS10985 (LTQ02_10985) coaE 2470290..2470904 (+) 615 WP_017088713.1 dephospho-CoA kinase -
  LTQ02_RS10990 (LTQ02_10990) zapD 2470931..2471671 (+) 741 WP_017087400.1 cell division protein ZapD -
  LTQ02_RS10995 (LTQ02_10995) yacG 2471753..2471950 (+) 198 WP_004735515.1 DNA gyrase inhibitor YacG -
  LTQ02_RS11000 (LTQ02_11000) rplS 2472302..2472655 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62092.73 Da        Isoelectric Point: 4.9258

>NTDB_id=635748 LTQ02_RS10970 WP_243578788.1 2466421..2468106(+) (pilB) [Vibrio splendidus strain 4_C08b]
MLTNLPTVLRQANLLSLTQEQAVAEHVQASGVSTPEALLVLDFFSGDSLANNIKTIFGLPLVRVANTDYEVLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEISDDEITSIEDLSQDDSPVSRFINQILVDAARKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCDHCKAADDSPDIFLRHSIPNSQI
IYKANSQGCNECNQGYSGRVGIYEVMPFSDQLKNSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=635748 LTQ02_RS10970 WP_243578788.1 2466421..2468106(+) (pilB) [Vibrio splendidus strain 4_C08b]
ATGCTAACCAACCTCCCCACGGTTCTACGTCAGGCTAATCTACTTAGTCTGACTCAAGAACAAGCTGTTGCGGAACATGT
ACAAGCTTCAGGTGTTTCGACGCCTGAAGCTTTGCTCGTTCTTGATTTCTTCAGCGGTGATTCCTTAGCAAACAATATAA
AAACCATCTTCGGCTTACCGTTGGTACGAGTTGCTAATACAGATTATGAAGTATTATGTGAACAACTAGGGCTTCGCGAA
CTGATCACCAAGTATCGCGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTTGCCTCAGCCGACCCAACCGACTT
ACAAGCTGAAGACGACTTTCGATTCGCGACAGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAGGCG
CAATACGAAAGCTGTATGGCCGTTCTATTTCCGGACAAGACTCCAAGCGTAAAGAGATCACTCAAGATGAGCTCGCTAAC
CTTGTTGAGATATCTGATGACGAGATAACTTCGATTGAAGACCTCAGCCAAGATGACTCCCCTGTCAGTCGCTTTATCAA
TCAGATTCTAGTCGATGCAGCACGTAAAGGCGCATCGGATATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAAATTCAACAGCCCGCCTCTCATTTAAGTCGTCGCCTATCGGCTCGCTTGAAGATT
CTCGCCAAACTCGATATCGCCGAACGTCGCTTACCTCAAGATGGTCGTATCAAGTTACGTTTAAATGACGAACTCGCGAT
TGATATGCGTGTATCGACGCTGCCAACATTATGGGGAGAAAAGATCGTTCTTCGTCTTCTAGACAGCAGCGCTGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGATCAAAAAGCACTCTACCTCAATGCATTGAAACGCCCACAAGGGATGATC
TTAATGACCGGGCCAACGGGCAGCGGTAAAACGGTTTCTCTTTATACTGGCCTTCGAGTACTCAACACCACTGAACGCAA
TATATCAACAGCCGAAGACCCTGTTGAAATTAACCTATGTGGTATCAATCAGGTACAAGTGACGCCTAAGATCGGCTTTG
GTTTCGCTGAAGCGTTGCGATCATTTTTACGACAAGATCCCGATGTGGTAATGGTCGGTGAGATCCGCGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACCGGACACCTCGTTCTTTCGACACTGCATACCAACTCCGCTGCAGAAACCGT
GACTCGACTCGCTCACATGGGAATTGAACCTTTTAATCTTGCTTCATCATTAAGCCTGATTATCGCCCAACGACTGGCAA
GACGATTGTGCGACCACTGTAAAGCCGCTGACGACTCACCTGATATATTTCTACGCCACTCTATTCCCAATAGCCAAATC
ATCTACAAAGCCAACTCACAAGGGTGTAATGAGTGTAATCAGGGATACTCGGGGCGAGTCGGTATCTATGAAGTCATGCC
GTTTAGCGACCAACTGAAAAATAGCCTGATAGACAAACCGAACGCGTTAGCCATTGAAAACTTGGCACGTCGAGAAGGCA
TGAGAACTTTACAAGAATCTGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.404

100

0.738
  pilB Vibrio campbellii strain DS40M4

70.285

100

0.704
  pilB Vibrio parahaemolyticus RIMD 2210633

69.751

100

0.699
  pilB Acinetobacter baumannii D1279779

50.482

92.513

0.467
  pilB Legionella pneumophila strain ERS1305867

50

90.909

0.455
  pilB Acinetobacter baylyi ADP1

48.94

92.513

0.453
  pilF Neisseria gonorrhoeae MS11

43.784

98.93

0.433