Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LSO60_RS14830 Genome accession   NZ_CP089051
Coordinates   3121087..3122808 (+) Length   573 a.a.
NCBI ID   WP_044434067.1    Uniprot ID   -
Organism   Acinetobacter ursingii strain RIVM_C010559     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3116087..3127808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO60_RS14795 (LSO60_14770) nusA 3116869..3118353 (-) 1485 WP_004991663.1 transcription termination factor NusA -
  LSO60_RS14800 (LSO60_14775) rimP 3118389..3118913 (-) 525 WP_263512502.1 ribosome maturation factor RimP -
  LSO60_RS14820 (LSO60_14795) secG 3119599..3119928 (-) 330 WP_004991669.1 preprotein translocase subunit SecG -
  LSO60_RS14825 (LSO60_14800) tpiA 3119938..3120735 (-) 798 WP_044434065.1 triose-phosphate isomerase -
  LSO60_RS14830 (LSO60_14805) pilB 3121087..3122808 (+) 1722 WP_044434067.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LSO60_RS14835 (LSO60_14810) pilC 3122839..3124065 (+) 1227 WP_044434069.1 type II secretion system F family protein Machinery gene
  LSO60_RS14840 pilD 3124065..3124925 (+) 861 WP_044434071.1 A24 family peptidase Machinery gene
  LSO60_RS14845 (LSO60_14825) coaE 3124928..3125521 (+) 594 WP_044434073.1 dephospho-CoA kinase -
  LSO60_RS14850 (LSO60_14830) - 3125503..3126426 (-) 924 WP_044434075.1 DMT family transporter -
  LSO60_RS14855 (LSO60_14835) rlmB 3126467..3127216 (-) 750 WP_263512503.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LSO60_RS14860 (LSO60_14840) - 3127336..3127662 (-) 327 WP_004991681.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 64475.17 Da        Isoelectric Point: 5.6966

>NTDB_id=634828 LSO60_RS14830 WP_044434067.1 3121087..3122808(+) (pilB) [Acinetobacter ursingii strain RIVM_C010559]
MADDELKSKYPTYLDRLVATGFLTKEQIHRMFYEINKNNKFIVQYLVEAFDIKPSIIAETISIEYEEPQFDLNTFNLSLI
PHGIIDEKLITKHKILPLSVKDHTLYVAVVDPSLTQDLDTIRFNSKKKLELVIVEHDKLERALEEVYGTNAQLEFLNSLH
NEDSENTDDILSEFSFDINADHEKDDTIVVKYLNKLLYDAIKMGASDLHFEPYAKIYRVRYRIDGVLHAISFPPLVLAPR
LTVRLKIMAKMDISERRSPQDGRIKLPVSQFKSIDFRVSSLPTLYGEKLVLRILDASSATLGIDALGYEEDQKALFLEAL
NKPQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGVNQVNVNPKVGLTFAAALKSFLRQDPDIIMVG
EIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNLATSVNLVIAQRLARRLCTKCKEPIQIPRNSLIE
MGFTEEDLSHPKLRIYQPVGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIADASEKLGFNNLRRSGLKKVMQG
VTSLQEVNRVTSE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=634828 LSO60_RS14830 WP_044434067.1 3121087..3122808(+) (pilB) [Acinetobacter ursingii strain RIVM_C010559]
ATGGCAGATGATGAGCTAAAGTCAAAATATCCAACTTACCTGGATCGCTTAGTTGCGACTGGATTTCTGACAAAAGAACA
GATCCATCGGATGTTTTATGAAATAAATAAAAATAACAAATTTATTGTTCAGTATCTTGTAGAAGCCTTTGATATAAAGC
CATCAATTATTGCTGAAACTATTTCTATTGAATATGAAGAACCTCAATTTGATCTTAATACATTTAATCTCTCTTTGATT
CCTCATGGTATTATCGATGAAAAATTAATCACAAAGCATAAAATTTTACCTCTAAGCGTAAAAGACCACACCTTATATGT
TGCTGTTGTAGACCCCTCCCTTACTCAAGATTTAGATACAATTCGCTTTAATAGTAAGAAAAAACTAGAACTTGTGATCG
TTGAACATGACAAATTGGAACGCGCACTTGAAGAAGTTTATGGTACAAATGCCCAGCTTGAATTTCTGAATAGTTTACAT
AATGAAGACAGCGAAAATACTGACGACATCTTAAGTGAGTTTAGCTTTGATATAAATGCAGACCATGAGAAAGACGACAC
TATTGTTGTTAAATATTTAAATAAACTGCTTTATGATGCAATTAAAATGGGGGCATCCGACCTTCATTTTGAACCTTATG
CCAAAATCTATCGCGTTAGATATCGTATCGATGGTGTTTTACATGCAATTTCATTCCCACCATTAGTACTCGCACCAAGA
CTAACTGTTCGCCTAAAAATTATGGCGAAGATGGATATCTCAGAACGGCGCTCTCCACAAGATGGTCGTATAAAATTACC
AGTCTCACAATTTAAATCTATCGATTTTCGTGTCAGCTCACTGCCTACATTATATGGTGAAAAATTAGTGCTACGTATTC
TAGATGCTTCTAGCGCAACATTAGGTATTGATGCGCTTGGCTACGAGGAAGATCAAAAAGCATTATTTTTAGAAGCACTG
AACAAGCCACAAGGTATGTTGCTAATTACAGGACCAACGGGTTCTGGTAAAACAGTTTCGTTATACACAGGCCTCAATAT
TCTTAATACTGAGAGTTCAAATATTTCCACAGCAGAAGATCCTGTGGAAATTAACCTTGAAGGTGTTAATCAGGTGAATG
TTAACCCCAAGGTGGGCTTGACCTTTGCCGCTGCGCTAAAGTCTTTTTTACGTCAGGATCCTGACATTATCATGGTGGGT
GAGATTCGTGATCTGGAAACAGCAGAAATCGCAATCAAAGCCGCACAAACGGGTCACATGGTCATGTCTACGCTGCATAC
TAACAGTGCGCCAGAAACATTAACACGTTTACGCAATATGGGCGTACCTTCATTTAATCTTGCAACGTCCGTGAACTTAG
TAATTGCTCAACGATTGGCCCGACGTCTTTGTACTAAATGTAAAGAGCCAATTCAGATCCCCAGAAATAGTTTGATCGAG
ATGGGGTTTACAGAGGAGGATCTATCTCACCCTAAACTCCGTATTTACCAACCTGTGGGATGTTCAGAATGCCGTGAAGG
CTACAAAGGTCGTGTCGGGATTTACGAAGTCATGAAAGTCACACCTGAAATTTCGAGAATTATTATGGAAGATGGAAATG
CAATACAAATTGCAGATGCATCGGAAAAACTGGGGTTTAATAATCTTCGTCGCTCAGGTCTTAAAAAGGTGATGCAAGGG
GTTACTTCTTTACAGGAAGTCAATCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

69.097

100

0.695
  pilB Acinetobacter baylyi ADP1

66.898

100

0.674
  pilB Legionella pneumophila strain ERS1305867

50.647

94.415

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

48.736

96.684

0.471
  pilF Neisseria gonorrhoeae MS11

47.857

97.731

0.468
  pilB Vibrio cholerae strain A1552

47.748

96.859

0.462
  pilB Vibrio campbellii strain DS40M4

47.491

97.382

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

33.981

100

0.366