Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LSO58_RS15040 Genome accession   NZ_CP089044
Coordinates   3176816..3178537 (+) Length   573 a.a.
NCBI ID   WP_004991672.1    Uniprot ID   A0A3F3LEM8
Organism   Acinetobacter ursingii strain RIVM_C010761     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3171816..3183537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO58_RS15005 (LSO58_14975) nusA 3172599..3174083 (-) 1485 WP_004991663.1 transcription termination factor NusA -
  LSO58_RS15010 (LSO58_14980) rimP 3174119..3174643 (-) 525 WP_004991666.1 ribosome maturation factor RimP -
  LSO58_RS15030 (LSO58_15000) secG 3175329..3175658 (-) 330 WP_004991669.1 preprotein translocase subunit SecG -
  LSO58_RS15035 (LSO58_15005) tpiA 3175668..3176465 (-) 798 WP_004991671.1 triose-phosphate isomerase -
  LSO58_RS15040 (LSO58_15010) pilB 3176816..3178537 (+) 1722 WP_004991672.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LSO58_RS15045 (LSO58_15015) pilC 3178568..3179794 (+) 1227 WP_004991675.1 type II secretion system F family protein Machinery gene
  LSO58_RS15050 pilD 3179794..3180654 (+) 861 WP_004991676.1 prepilin peptidase Machinery gene
  LSO58_RS15055 (LSO58_15030) coaE 3180657..3181250 (+) 594 WP_004991677.1 dephospho-CoA kinase -
  LSO58_RS15060 (LSO58_15035) - 3181232..3182155 (-) 924 WP_004991678.1 DMT family transporter -
  LSO58_RS15065 (LSO58_15040) rlmB 3182196..3182945 (-) 750 WP_004991679.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LSO58_RS15070 (LSO58_15045) - 3183065..3183391 (-) 327 WP_004991681.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 64463.18 Da        Isoelectric Point: 5.6966

>NTDB_id=634788 LSO58_RS15040 WP_004991672.1 3176816..3178537(+) (pilB) [Acinetobacter ursingii strain RIVM_C010761]
MADDELKSKYPTYLDRLVATGFLTKEQIHRMFYEINKNNKFIVQYLVEAFDIKPSIIAEAISIEYEEPQFDLNTFNLSLI
PHGIIDEKLITKHKILPLSVKDHTLYVAVVDPSLTQDLDTIRFNSKKKLELVIVEHDKLERALEEVYGTNAQLEFLNSLH
NEDSENTDDILSEFSFDINADHEKDDTIVVKYLNKMLYDAIKMGASDLHFEPYAKIYRVRYRIDGVLHAISFPPLVLAPR
LTVRLKIMAKMDISERRSPQDGRIKLPVSQFKSIDFRVSSLPTLYGEKLVLRILDASSATLGIDALGYEEDQKALFLEAL
NKPQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGVNQVNVNPKVGLTFAAALKSFLRQDPDIIMVG
EIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNLATSVNLVIAQRLARRLCSKCKEPIQIPRNSLIE
MGFTEEDLSHPKLRIYQPVGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIADASEKLGFNNLRRSGLKKVMQG
ITSLQEVNRVTSE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=634788 LSO58_RS15040 WP_004991672.1 3176816..3178537(+) (pilB) [Acinetobacter ursingii strain RIVM_C010761]
ATGGCAGATGATGAGCTAAAGTCAAAATATCCAACTTACCTGGATCGCTTAGTTGCGACTGGATTTCTGACAAAAGAACA
GATCCATCGGATGTTTTATGAAATAAATAAAAATAACAAATTTATTGTTCAGTATCTTGTAGAAGCCTTTGATATAAAGC
CATCAATTATTGCTGAAGCTATTTCTATTGAATATGAAGAACCTCAATTTGATCTTAATACATTTAATCTCTCTTTGATT
CCTCATGGTATTATCGATGAAAAATTAATCACAAAGCATAAAATTTTACCTCTAAGCGTAAAAGACCACACCTTATATGT
TGCTGTTGTAGACCCCTCTCTTACTCAAGATTTAGATACAATTCGCTTTAATAGTAAGAAAAAACTAGAACTTGTGATCG
TTGAACATGACAAATTGGAACGCGCACTTGAAGAGGTTTATGGTACAAATGCCCAACTTGAATTTCTGAATAGTTTACAT
AATGAAGACAGCGAAAATACTGACGACATCTTAAGTGAGTTTAGCTTTGATATAAATGCAGACCATGAGAAAGACGACAC
TATTGTTGTTAAATATTTAAATAAAATGCTTTATGATGCAATTAAAATGGGGGCATCCGACCTTCATTTTGAACCTTATG
CCAAAATCTATCGCGTTAGATATCGTATCGATGGTGTTTTACATGCAATTTCATTCCCACCATTAGTACTCGCACCAAGA
CTAACTGTTCGCCTAAAAATTATGGCGAAGATGGATATCTCAGAACGGCGCTCTCCACAAGATGGTCGTATAAAATTACC
AGTCTCACAATTTAAATCTATCGATTTTCGTGTCAGCTCACTGCCTACATTATATGGTGAAAAATTAGTGCTACGTATTC
TGGATGCTTCTAGCGCAACATTGGGTATTGATGCGCTTGGCTACGAGGAAGATCAAAAAGCATTATTTTTAGAAGCACTG
AACAAGCCACAAGGTATGTTGCTAATTACAGGACCAACGGGTTCTGGTAAAACAGTTTCGTTATACACAGGCCTCAATAT
TCTTAATACTGAGAGTTCAAATATTTCCACAGCAGAAGATCCTGTGGAAATTAACCTTGAAGGTGTTAATCAGGTGAATG
TTAACCCCAAGGTGGGCTTGACCTTTGCCGCTGCGCTAAAGTCTTTTTTACGTCAGGATCCTGACATTATCATGGTGGGT
GAGATTCGTGATCTGGAAACAGCAGAAATCGCAATCAAAGCCGCACAAACGGGTCACATGGTCATGTCTACGCTGCATAC
TAACAGTGCGCCAGAAACATTAACACGTTTACGCAATATGGGCGTACCTTCATTTAATCTTGCAACGTCCGTGAACTTAG
TAATTGCTCAACGATTGGCCCGACGTCTTTGTAGTAAATGTAAAGAGCCAATTCAGATCCCCAGAAATAGTTTGATCGAG
ATGGGGTTTACAGAGGAGGATCTATCTCACCCTAAACTCCGTATTTACCAACCTGTGGGATGTTCAGAATGCCGTGAAGG
CTACAAAGGTCGTGTCGGGATTTACGAAGTCATGAAAGTCACACCTGAAATTTCGAGAATTATTATGGAAGATGGAAATG
CAATACAAATTGCAGATGCATCGGAAAAACTGGGGTTTAATAATCTTCGTCGCTCAGGTCTTAAAAAGGTGATGCAAGGG
ATTACTTCTTTACAGGAAGTCAATCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3F3LEM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.75

100

0.691
  pilB Acinetobacter baylyi ADP1

66.724

100

0.672
  pilB Legionella pneumophila strain ERS1305867

50.647

94.415

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

48.917

96.684

0.473
  pilF Neisseria gonorrhoeae MS11

48.214

97.731

0.471
  pilB Vibrio cholerae strain A1552

47.928

96.859

0.464
  pilB Vibrio campbellii strain DS40M4

47.67

97.382

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

33.981

100

0.366