Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LRO84_RS16900 Genome accession   NZ_CP088955
Coordinates   3575277..3576989 (+) Length   570 a.a.
NCBI ID   WP_231762042.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain ARu19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3570277..3581989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO84_RS16865 (LRO84_16865) nusA 3571163..3572647 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  LRO84_RS16870 (LRO84_16870) rimP 3572684..3573208 (-) 525 WP_005037183.1 ribosome maturation factor RimP -
  LRO84_RS16890 (LRO84_16890) secG 3573849..3574178 (-) 330 WP_231762040.1 preprotein translocase subunit SecG -
  LRO84_RS16895 (LRO84_16895) tpiA 3574191..3574985 (-) 795 WP_231762041.1 triose-phosphate isomerase -
  LRO84_RS16900 (LRO84_16900) pilB 3575277..3576989 (+) 1713 WP_231762042.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LRO84_RS16905 (LRO84_16905) pilC 3577017..3578243 (+) 1227 WP_004640845.1 type II secretion system F family protein Machinery gene
  LRO84_RS18730 pilD 3578243..3579103 (+) 861 WP_269449639.1 prepilin peptidase Machinery gene
  LRO84_RS16920 (LRO84_16920) coaE 3579105..3579701 (+) 597 WP_003654573.1 dephospho-CoA kinase -
  LRO84_RS16925 (LRO84_16925) - 3579698..3580612 (-) 915 WP_231762043.1 DMT family transporter -
  LRO84_RS16930 (LRO84_16930) rlmB 3580646..3581395 (-) 750 WP_005037200.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LRO84_RS16935 (LRO84_16935) - 3581498..3581824 (-) 327 WP_003654567.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63522.84 Da        Isoelectric Point: 5.0228

>NTDB_id=634221 LRO84_RS16900 WP_231762042.1 3575277..3576989(+) (pilB) [Acinetobacter calcoaceticus strain ARu19]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDTAKRAKQDTVSYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILRHRVLPLVQRGQILYVATSNPSNIEAIDAIRFNSKLMVEQVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLDTSAAPEEDDDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPSDIPKQSLLEMGF
TEQDLTHPDFQVFQPIGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFSNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=634221 LRO84_RS16900 WP_231762042.1 3575277..3576989(+) (pilB) [Acinetobacter calcoaceticus strain ARu19]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATACAGCAAAAAGAGCTAAACAGGATACAGTTTCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAGATT
CCTAAAGATTTGGTCGACCAGAAACTGATTTTAAGACATCGGGTCTTACCTCTCGTACAAAGAGGACAGATCTTATATGT
TGCAACCAGTAATCCCAGCAATATTGAAGCGATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAGCAGGTTATTG
TCGAGCACCATAAACTTGAGAAAATTCTGGCGCAGCAATTTACTGAAGAAAGTAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTTGATACCTCAGCCGCTCCAGAAGAGGATGATGACACTCCTCAGGGCGATGAAGCACCTATTGT
TAAATATGTTAACAAGTTATTAATAGATGCGATTCGTATGGGAGCTTCAGACTTGCATTTCGAACCCTACGAGAAATCGT
ATCGGGTCCGTTATCGGGTCGATGGCGTATTGCGTCAGATTGCAAACCCACCTTTGCAACTCGCCACTCGGTTAGCTTCA
CGTTTAAAAGTTATGTCTCAAATGGACATTTCTGAAAAGCGTGTTCCTCAAGATGGTCGTATCAAACTCAAGCTATCAAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACTTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CTAGTGCCATGCTGGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCACTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATTACTGGTCCTACAGGTTCTGGTAAAACAGTATCCTTATATACGGGCCTCAACATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGTATTAATCAGGTTAACGTAAACCCTA
AAGTAGGTCTCACTTTTTCGGCTGCGCTTAAATCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAGGCTGCTCAGACGGGTCACATGGTAATGTCCACACTACATACCAACAGTGC
CCCTGAAACACTTACTCGTCTACGCAATATGGGAGTTCCATCTTTCAATATTGCGACATCAGTCAATCTGGTCATTGCTC
AACGTTTGGCACGTCGATTATGCCCTCAATGTAAGGTGCCTAGTGACATTCCTAAACAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGATCTAACACATCCCGATTTTCAAGTTTTTCAACCGATCGGTTGTCCTGAGTGTCGTGAGGGCTACAAAGG
TCGAGTAGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAATTGGGGTTTAGTAATCTGCGTCGCTCAGGTTTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

93.158

100

0.932
  pilB Acinetobacter baylyi ADP1

78.596

100

0.786
  pilB Legionella pneumophila strain ERS1305867

52.456

100

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

51.692

93.333

0.482
  pilF Neisseria gonorrhoeae MS11

48.754

98.596

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

49.904

91.053

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

35.993

100

0.388