Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LRO55_RS16900 Genome accession   NZ_CP088954
Coordinates   3589113..3590825 (+) Length   570 a.a.
NCBI ID   WP_231764178.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain ACa13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3584113..3595825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO55_RS16865 (LRO55_16865) nusA 3584999..3586483 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  LRO55_RS16870 (LRO55_16870) rimP 3586520..3587044 (-) 525 WP_005037183.1 ribosome maturation factor RimP -
  LRO55_RS16890 (LRO55_16890) secG 3587685..3588014 (-) 330 WP_005037186.1 preprotein translocase subunit SecG -
  LRO55_RS16895 (LRO55_16895) tpiA 3588027..3588821 (-) 795 WP_003654580.1 triose-phosphate isomerase -
  LRO55_RS16900 (LRO55_16900) pilB 3589113..3590825 (+) 1713 WP_231764178.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LRO55_RS16905 (LRO55_16905) pilC 3590852..3592078 (+) 1227 WP_231764179.1 type II secretion system F family protein Machinery gene
  LRO55_RS18755 pilD 3592078..3592938 (+) 861 WP_080026205.1 prepilin peptidase Machinery gene
  LRO55_RS16920 (LRO55_16920) coaE 3592940..3593536 (+) 597 WP_004640841.1 dephospho-CoA kinase -
  LRO55_RS16925 (LRO55_16925) - 3593533..3594447 (-) 915 WP_231764180.1 DMT family transporter -
  LRO55_RS16930 (LRO55_16930) rlmB 3594482..3595231 (-) 750 WP_005037200.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LRO55_RS16935 (LRO55_16935) - 3595334..3595660 (-) 327 WP_003654567.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63523.82 Da        Isoelectric Point: 5.0228

>NTDB_id=634174 LRO55_RS16900 WP_231764178.1 3589113..3590825(+) (pilB) [Acinetobacter calcoaceticus strain ACa13]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVSYLIEEVHISPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLVQRGQILYVATSNPSNIEAIDTIRFNSKLMVEQVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLDTSTAPEEDDDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPSDIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFSNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=634174 LRO55_RS16900 WP_231764178.1 3589113..3590825(+) (pilB) [Acinetobacter calcoaceticus strain ACa13]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACAGGATACAGTTTCGTACCTGATTGAAGAGGTTCATATCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTATGATACCAGCCAGATT
CCTAAAGATTTGGTCGACCAGAAACTGATTTTAAAACATCGGGTCTTACCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCTACCAGTAATCCCAGTAACATTGAAGCGATTGATACTATTCGCTTCAACAGCAAGCTTATGGTTGAGCAGGTTATTG
TCGAGCATCATAAACTTGAGAAAATTCTGGCGCAGCAATTTACTGAAGAAAGTAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTTGATACCTCAACCGCTCCAGAAGAGGATGATGACACTCCTCAGGGCGATGAAGCACCTATTGT
TAAATATGTTAACAAGTTATTAATAGATGCGATTCGTATGGGGGCTTCAGACTTGCATTTCGAACCCTATGAGAAATCGT
ATCGGGTCCGTTATCGGGTCGATGGCGTTTTGCGTCAAATTGCAAATCCGCCTTTGCAACTGGCTAACCGTTTGGCTTCA
CGCCTGAAAGTTATGTCTCAAATGGACATTTCCGAAAAGCGTGTTCCTCAAGATGGTCGTATCAAGCTCAAGCTATCGAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CTAGTGCCATGTTAGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCACTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATTACTGGTCCTACGGGTTCTGGTAAAACAGTATCCTTATATACGGGCCTCAACATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGATCCGGTCGAAATTAACCTTGAAGGTATTAATCAGGTTAACGTAAACCCTA
AAGTAGGTCTCACTTTTTCGGCTGCGCTTAAATCATTTTTACGTCAGGATCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAGGCTGCTCAGACGGGTCACATGGTAATGTCCACACTACATACCAACAGTGC
CCCTGAAACACTTACTCGTCTACGCAATATGGGAGTTCCATCTTTTAATATTGCGACATCGGTCAATTTGGTAATTGCTC
AACGTTTGGCACGTCGATTATGCCCTCAATGTAAGGTGCCTAGTGACATTCCTAAACAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGATCTAACACATCCCGATTTTCAAGTTTTTCAACCGGTCGGTTGTCCTGAGTGTCGTGAGGGCTACAAAGG
TCGAGTAGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAATTGGGGTTTAGTAATCTGCGTCGCTCAGGTTTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

93.684

100

0.937
  pilB Acinetobacter baylyi ADP1

78.947

100

0.789
  pilB Legionella pneumophila strain ERS1305867

52.189

100

0.523
  pilF Neisseria gonorrhoeae MS11

49.11

98.596

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

51.504

93.333

0.481
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB Vibrio cholerae strain A1552

49.711

91.053

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384