Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LQ335_RS16715 Genome accession   NZ_CP088244
Coordinates   3629045..3630775 (-) Length   576 a.a.
NCBI ID   WP_134968484.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain 2013-SM4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3624045..3635775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ335_RS16700 (LQ335_16700) sucC 3624247..3625416 (-) 1170 WP_005418614.1 ADP-forming succinate--CoA ligase subunit beta -
  LQ335_RS16705 (LQ335_16705) - 3625824..3627437 (+) 1614 WP_134968485.1 ATP-binding protein -
  LQ335_RS16710 (LQ335_16710) pilR 3627498..3628871 (+) 1374 WP_075676419.1 sigma-54 dependent transcriptional regulator Regulator
  LQ335_RS16715 (LQ335_16715) pilB 3629045..3630775 (-) 1731 WP_134968484.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LQ335_RS16720 (LQ335_16720) - 3631000..3631407 (-) 408 WP_134968489.1 pilin -
  LQ335_RS16725 (LQ335_16725) pilA/pilAI 3631524..3631940 (-) 417 WP_134968483.1 pilin Machinery gene
  LQ335_RS16730 (LQ335_16730) pilC 3632297..3633556 (+) 1260 WP_134968482.1 type II secretion system F family protein Machinery gene
  LQ335_RS16735 (LQ335_16735) - 3633564..3634427 (+) 864 WP_065185263.1 A24 family peptidase -
  LQ335_RS16740 (LQ335_16740) coaE 3634439..3635050 (+) 612 WP_134968481.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62195.12 Da        Isoelectric Point: 5.8733

>NTDB_id=633439 LQ335_RS16715 WP_134968484.1 3629045..3630775(-) (pilB) [Stenotrophomonas maltophilia strain 2013-SM4]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGIGNLDVSGGDEDISGGGDAGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPANLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=633439 LQ335_RS16715 WP_134968484.1 3629045..3630775(-) (pilB) [Stenotrophomonas maltophilia strain 2013-SM4]
ATGAACGCCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGACGCCATGGCCAAGGCCGCTGCGGCCCGCCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TTGGGGCGGCCCAGTTGGCGGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAAGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTGGGTACCAGCAACCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAACCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCGCTCGGTGGTGAC
GACGATGGCATTGGTAACCTGGATGTGTCCGGCGGTGACGAGGACATTTCCGGGGGCGGCGACGCCGGCATCGATGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGACGACTACCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTGAAG
TTGAACCAGCGCATTGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAACCGGACCAGCAGAAGCTGTTC
CTGGAGGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCCGCGGCACTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTTGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGGCTGTGCAACAACTGCAAGCGCCCGGCCAATCTGCCCGAGCAT
GCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGACGCGGGTATCGAGCTGTACGAGGCGGTCGGCTGCGATGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGTTGGCTG
GCGGCAATGCGTTGCAGATTGCCGAGGCCGCGCAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCT
GCTGCGGGCATTACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.268

100

0.556
  pilB Acinetobacter baylyi ADP1

54.623

100

0.554
  pilB Legionella pneumophila strain ERS1305867

52.098

99.306

0.517
  pilF Neisseria gonorrhoeae MS11

49.12

98.611

0.484
  pilB Vibrio cholerae strain A1552

48.427

99.306

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

44.965

100

0.45
  pilB Vibrio campbellii strain DS40M4

45.343

98.785

0.448