Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LQZ07_RS05170 Genome accession   NZ_CP088080
Coordinates   1102692..1104401 (-) Length   569 a.a.
NCBI ID   WP_152780580.1    Uniprot ID   A0A7C9G2U3
Organism   Agarivorans sp. B2Z047     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1097692..1109401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQZ07_RS05140 (LQZ07_05140) mutT 1098465..1098869 (+) 405 WP_249483546.1 8-oxo-dGTP diphosphatase MutT -
  LQZ07_RS05145 (LQZ07_05145) yacG 1098872..1099072 (-) 201 WP_016400794.1 DNA gyrase inhibitor YacG -
  LQZ07_RS05150 (LQZ07_05150) zapD 1099072..1099812 (-) 741 WP_152780588.1 cell division protein ZapD -
  LQZ07_RS05155 (LQZ07_05155) coaE 1099871..1100473 (-) 603 WP_249483548.1 dephospho-CoA kinase -
  LQZ07_RS05160 (LQZ07_05160) pilD 1100470..1101351 (-) 882 WP_152780584.1 A24 family peptidase Machinery gene
  LQZ07_RS05165 (LQZ07_05165) pilC 1101354..1102604 (-) 1251 WP_152780582.1 type II secretion system F family protein Machinery gene
  LQZ07_RS05170 (LQZ07_05170) pilB 1102692..1104401 (-) 1710 WP_152780580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LQZ07_RS05175 (LQZ07_05175) - 1104408..1104896 (-) 489 WP_152780579.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LQZ07_RS05180 (LQZ07_05180) nadC 1105150..1105989 (-) 840 WP_152780577.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LQZ07_RS05185 (LQZ07_05185) ampD 1106163..1106699 (+) 537 WP_152780575.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LQZ07_RS05190 (LQZ07_05190) ampE 1106774..1107628 (+) 855 WP_152780573.1 beta-lactamase regulator AmpE -
  LQZ07_RS05195 (LQZ07_05195) pdhR 1107885..1108649 (+) 765 WP_016400804.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62933.32 Da        Isoelectric Point: 4.9073

>NTDB_id=632572 LQZ07_RS05170 WP_152780580.1 1102692..1104401(-) (pilB) [Agarivorans sp. B2Z047]
MTNSIKELNGLASSLAVSFPEKQEQLVELYKRSVKEQRSFTTVLVENELISSRELADFCEAQFGIPLLDLDAIELSEIPQ
SFLNEKLIFKHHVLPVYSQNQTLYVAMADPSNVTALEDFGFSFGLHVEALLVEETKLLKAIQSLVDDTANLDLGDISDDD
ISDLEVSEENSRLDEKQNAKEDDAPIVVYINKILMDSIRRGASDLHFEPYEHKYRIRFRIDGILHEIASPPINLANRFSA
RLKVMARMDIAERRLPQDGRIKLKTGRNKAIDMRVNSLPTMWGEKIVIRILDSSAANLNIDILGYDDKQKKLYLDALARP
QGMILVTGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLPGVNQVQINNKAGMTFASALRAFLRQDPDIVMVGEIR
DLETAEISIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAFNIASSVSLIIAQRLARRLCEKCKKPKILEAELLPKIGF
SQEQLNAGVKLFDPVGCDECTGGFKGRVGIYEVMPMSTAISKIIMAEGNSIEIAEQAVAEGMYSLRMSALEKARLGVTSL
SEVERVTNV

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=632572 LQZ07_RS05170 WP_152780580.1 1102692..1104401(-) (pilB) [Agarivorans sp. B2Z047]
ATGACTAACAGCATCAAAGAATTAAACGGCCTAGCTTCTAGCTTGGCCGTTTCTTTTCCAGAAAAACAAGAGCAATTAGT
TGAACTATACAAGCGCAGCGTTAAGGAACAACGTTCTTTTACAACAGTGCTAGTAGAAAATGAGCTTATTTCTAGTAGAG
AACTAGCCGACTTTTGCGAGGCGCAATTTGGAATCCCACTACTCGATTTAGATGCGATTGAACTTAGCGAAATCCCGCAA
AGCTTCCTCAATGAAAAGCTGATATTTAAACATCACGTATTACCAGTATATAGCCAAAACCAAACTCTTTATGTAGCCAT
GGCCGACCCAAGTAATGTTACGGCACTCGAAGATTTTGGTTTTAGTTTTGGACTGCACGTTGAAGCTTTATTGGTTGAAG
AAACCAAGTTACTCAAGGCTATTCAATCCCTTGTTGATGATACGGCCAATCTCGATTTGGGCGATATATCCGATGATGAC
ATCAGCGATTTAGAAGTTAGTGAAGAGAACTCGCGTTTAGATGAAAAGCAAAACGCTAAAGAAGATGATGCACCAATTGT
TGTTTACATCAACAAAATATTGATGGACTCAATACGCCGTGGTGCTTCCGATTTGCATTTCGAACCTTACGAGCACAAAT
ACCGGATCCGTTTTCGAATAGACGGTATCCTGCACGAAATCGCCAGTCCACCGATTAACTTAGCTAACCGCTTTTCGGCT
CGCCTAAAAGTAATGGCGCGAATGGATATCGCCGAGAGGCGTTTACCTCAAGATGGCCGGATTAAGCTAAAAACTGGTCG
CAACAAAGCCATTGATATGCGGGTTAACTCTCTACCTACCATGTGGGGAGAGAAAATTGTAATTCGTATTCTCGACTCAT
CGGCTGCAAATCTAAATATTGATATTCTTGGTTATGACGACAAACAGAAGAAGCTCTACTTAGATGCTCTTGCTCGTCCA
CAAGGGATGATTCTGGTTACCGGGCCTACTGGCTCCGGTAAAACAGTATCTTTGTATACTGGCTTAAATATTCTAAATAC
CGTAGAAACTAATATTTCTACCGCAGAAGACCCTGTAGAAATTAATTTACCCGGGGTTAACCAAGTACAAATCAATAACA
AGGCGGGCATGACCTTCGCCAGCGCTTTGCGCGCCTTCCTTCGTCAAGACCCCGACATTGTAATGGTAGGTGAGATTCGA
GATTTAGAAACTGCTGAAATCTCGATTAAAGCGGCGCAAACCGGTCACTTAGTATTATCTACCTTACACACTAACTCTGC
GGCGGAAACTTTAACCCGTTTGCTCAATATGGGCGTGCCCGCCTTTAATATTGCATCTTCAGTAAGTTTGATTATTGCCC
AGCGACTGGCTCGACGTTTATGCGAGAAATGTAAAAAACCTAAGATATTAGAAGCTGAGTTACTACCTAAAATTGGTTTT
AGCCAAGAGCAACTAAATGCCGGAGTCAAGCTATTTGATCCTGTGGGTTGTGACGAATGCACTGGTGGTTTCAAAGGCCG
AGTAGGTATTTACGAAGTAATGCCTATGAGTACCGCCATATCAAAAATTATTATGGCTGAAGGTAATTCAATAGAAATCG
CTGAACAAGCTGTGGCAGAAGGCATGTACTCCCTTCGTATGTCAGCGTTAGAAAAAGCCCGCCTAGGCGTAACTAGCCTT
TCAGAGGTGGAGCGAGTCACAAATGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7C9G2U3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.795

92.443

0.534
  pilB Acinetobacter baylyi ADP1

55.288

94.728

0.524
  pilB Legionella pneumophila strain ERS1305867

52.305

99.121

0.518
  pilB Vibrio cholerae strain A1552

54.135

93.497

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

52.372

96.309

0.504
  pilB Vibrio campbellii strain DS40M4

51.906

96.837

0.503
  pilF Neisseria gonorrhoeae MS11

47.978

95.606

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.986

100

0.392
  pilB/pilB1 Synechocystis sp. PCC 6803

39.098

93.497

0.366