Detailed information NTG0063214

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: SAGCMC97051_RS04750
• Genome accession: NZ_AP020310
• Coordinates: 857794..858447 (+)
• Length: 217 a.a.
• NCBI ID: WP_000461744.1
• Uniprot ID: Q8E0F2
• Organism: Streptococcus agalactiae strain GCMC97051
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 852794..863447

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAGCMC97051_RS04725 (SAGCMC97051_08560)	-	852960..853790 (+)	831	WP_000735504.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
SAGCMC97051_RS04730 (SAGCMC97051_08570)	-	854025..855611 (+)	1587	WP_000673089.1	DEAD/DEAH box helicase	-
SAGCMC97051_RS04735 (SAGCMC97051_08580)	-	855796..856062 (-)	267	WP_000598736.1	GIY-YIG nuclease family protein	-
SAGCMC97051_RS04740 (SAGCMC97051_08590)	-	856055..856819 (-)	765	WP_000567425.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
SAGCMC97051_RS04745 (SAGCMC97051_08600)	-	856954..857694 (+)	741	WP_000500220.1	lysophospholipid acyltransferase family protein	-
SAGCMC97051_RS04750 (SAGCMC97051_08610)	comEA/celA/cilE	857794..858447 (+)	654	WP_000461744.1	helix-hairpin-helix domain-containing protein	Machinery gene
SAGCMC97051_RS04755 (SAGCMC97051_08620)	comEC/celB	858431..860668 (+)	2238	WP_000939903.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SAGCMC97051_RS04760 (SAGCMC97051_08630)	-	860794..861603 (+)	810	WP_000153219.1	Cof-type HAD-IIB family hydrolase	-
SAGCMC97051_RS04765 (SAGCMC97051_08640)	-	861614..862558 (+)	945	WP_000200830.1	LacI family DNA-binding transcriptional regulator	-

Sequence

Protein

• Length: 217 a.a.
• Molecular weight: 23713.07 Da
• Isoelectric Point: 9.5543

>NTDB_id=63214 SAGCMC97051_RS04750 WP_000461744.1 857794..858447(+) (comEA/celA/cilE) [Streptococcus agalactiae strain GCMC97051]
MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA
VNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID

Nucleotide

• Length: 654 bp

>NTDB_id=63214 SAGCMC97051_RS04750 WP_000461744.1 857794..858447(+) (comEA/celA/cilE) [Streptococcus agalactiae strain GCMC97051]
ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTATTTGG
AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACAATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA
TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA
GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAAGCAA
TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA
AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATCGCTACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA
GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTGGGGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA
AGGTGGTTTTAAAACTATTGATGACCTCAAAAACGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG
TTACAATTGACTAA

Domains

Predicted by InterProScan.

• SLBB domain (SLBB) (75-128)

• Helix-hairpin-helix motif (HHH_3) (153-215)

3D structure

• AlphaFold DB: Q8E0F2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	45	100	0.456
comEA/celA/cilE	Streptococcus pneumoniae Rx1	43.694	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae D39	43.694	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae R6	43.694	100	0.447
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.243	100	0.442
comEA/celA/cilE	Streptococcus mitis SK321	43.636	100	0.442
comEA	Lactococcus lactis subsp. cremoris KW2	39.013	100	0.401
comEA	Streptococcus thermophilus LMD-9	54.545	70.968	0.387