Detailed information NTG0631011

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LPH63_RS06410
• Genome accession: NZ_CP087695
• Coordinates: 1542513..1543643 (+)
• Length: 376 a.a.
• NCBI ID: WP_038272110.1
• Uniprot ID: Z9JI39
• Organism: Xylella taiwanensis strain PLS434
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1537513..1548643

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPH63_RS06400 (LPH63_06400)	-	1538865..1539716 (-)	852	WP_081755470.1	glycosyltransferase family 2 protein	-
LPH63_RS06405 (LPH63_06405)	pilT	1541321..1542355 (+)	1035	WP_038272165.1	type IV pilus twitching motility protein PilT	Machinery gene
LPH63_RS06410 (LPH63_06410)	pilU	1542513..1543643 (+)	1131	WP_038272110.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LPH63_RS06415 (LPH63_06415)	-	1543874..1544772 (-)	899	Protein_1272	WYL domain-containing protein	-
LPH63_RS06420 (LPH63_06420)	-	1545137..1545730 (+)	594	WP_230430827.1	hypothetical protein	-
LPH63_RS06425 (LPH63_06425)	-	1545907..1546716 (+)	810	WP_038272108.1	DUF481 domain-containing protein	-
LPH63_RS06430 (LPH63_06430)	hemC	1546754..1547671 (-)	918	WP_038272106.1	hydroxymethylbilane synthase	-
LPH63_RS06435 (LPH63_06435)	-	1547679..1548437 (-)	759	WP_038272104.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42121.51 Da
• Isoelectric Point: 6.7724

>NTDB_id=631011 LPH63_RS06410 WP_038272110.1 1542513..1543643(+) (pilU) [Xylella taiwanensis strain PLS434]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKMHGNITPITQVPLTSDQSRDMVFNVMTQAQREEFEKTHECNFAIDITN
IGRFRVNAFYQRSQIGMVLRRIEMSIPTFEKLTLPHVIKNLVMAKRGIIIIAGATGSGKSTSMASMVNYRNQNSTGHIIT
IEDPIEFMHKHENCIITQREIGTDTDSWENALKNTLRQAPDVIMIGEVRTQKTMDHAISFAETGHLVMCTLHANNANQAI
DRVINFFPEDRRGQLLMDLSLNLKGIIAQQLIQTPDGKGRCLAVEILLGTPLVQDYIRAGEIHKLKEIMQSSTNLGMKTF
DQSLVELYQAGAISHADALRYADSQNEVRLRIKLSEGKDAKTLEQGFDGIEIAEVS

Nucleotide

• Length: 1131 bp

>NTDB_id=631011 LPH63_RS06410 WP_038272110.1 1542513..1543643(+) (pilU) [Xylella taiwanensis strain PLS434]
ATGACGACCATCGATTTCACGTCCTTACTTAAGCTGATGGCGCACCAAAAGGCGTCAGACTTGTTTATCACATGTGGGAT
GCCACCATCGATGAAGATGCATGGCAATATCACTCCAATAACACAGGTACCGCTGACTTCAGACCAAAGCCGTGACATGG
TCTTCAATGTGATGACTCAGGCTCAGCGTGAAGAATTCGAGAAGACACACGAATGCAATTTCGCCATCGACATCACGAAC
ATTGGCCGCTTCCGCGTCAACGCATTCTATCAGCGCAGCCAGATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACGTTTGAGAAACTGACTCTCCCCCATGTGATCAAGAATCTCGTCATGGCAAAGCGTGGCATCATCATCATCGCTGGCG
CCACTGGCAGCGGCAAATCAACCTCAATGGCATCAATGGTCAACTACCGTAATCAAAACTCCACGGGGCATATCATCACT
ATCGAAGATCCCATTGAGTTCATGCACAAGCATGAGAACTGCATCATCACCCAACGCGAAATCGGTACCGATACCGATAG
TTGGGAAAACGCACTGAAAAACACTCTACGCCAGGCCCCAGATGTGATTATGATTGGTGAGGTACGTACCCAGAAAACGA
TGGACCACGCGATCTCCTTCGCTGAAACCGGCCATCTGGTAATGTGCACCTTGCATGCCAACAACGCCAATCAGGCAATA
GACCGTGTCATCAACTTCTTTCCGGAAGATCGGCGCGGTCAATTATTGATGGATCTGTCACTAAACCTAAAGGGCATCAT
CGCTCAACAATTAATCCAAACTCCGGATGGTAAAGGTCGCTGTTTAGCGGTGGAAATTTTGCTTGGCACTCCATTAGTCC
AAGACTACATTCGCGCAGGCGAGATCCACAAGCTCAAGGAAATCATGCAAAGCTCCACTAACCTTGGAATGAAGACTTTC
GATCAAAGCCTGGTAGAGCTGTATCAGGCCGGTGCGATTAGCCATGCAGATGCGCTACGCTATGCTGATTCGCAGAATGA
GGTACGCCTGCGCATTAAGCTCTCTGAGGGTAAAGACGCTAAAACCCTGGAGCAAGGATTCGATGGAATAGAGATTGCAG
AAGTGTCATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-272)

3D structure

• AlphaFold DB: Z9JI39

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.035	97.606	0.596
pilU	Acinetobacter baylyi ADP1	61.582	94.149	0.58
pilU	Vibrio cholerae strain A1552	52.247	94.681	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.408	94.415	0.391
pilT	Acinetobacter nosocomialis M2	39.826	91.489	0.364
pilT	Acinetobacter baumannii D1279779	39.826	91.489	0.364
pilT	Acinetobacter baumannii strain A118	39.826	91.489	0.364