Detailed information NTG0630969

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LPH51_RS11255
• Genome accession: NZ_CP087692
• Coordinates: 2689314..2690444 (-)
• Length: 376 a.a.
• NCBI ID: WP_038272110.1
• Uniprot ID: Z9JI39
• Organism: Xylella taiwanensis strain PLS427
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2684314..2695444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPH51_RS11230 (LPH51_11230)	-	2684520..2685278 (+)	759	WP_038272104.1	LytTR family DNA-binding domain-containing protein	-
LPH51_RS11235 (LPH51_11235)	hemC	2685286..2686203 (+)	918	WP_038272106.1	hydroxymethylbilane synthase	-
LPH51_RS11240 (LPH51_11240)	-	2686241..2687050 (-)	810	WP_038272108.1	DUF481 domain-containing protein	-
LPH51_RS11245 (LPH51_11245)	-	2687227..2688027 (-)	801	WP_230428206.1	hypothetical protein	-
LPH51_RS11250 (LPH51_11250)	-	2688185..2689083 (+)	899	Protein_2221	WYL domain-containing protein	-
LPH51_RS11255 (LPH51_11255)	pilU	2689314..2690444 (-)	1131	WP_038272110.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LPH51_RS11260 (LPH51_11260)	pilT	2690602..2691636 (-)	1035	WP_038272165.1	type IV pilus twitching motility protein PilT	Machinery gene
LPH51_RS11265 (LPH51_11265)	-	2693241..2694092 (+)	852	WP_081755470.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42121.51 Da
• Isoelectric Point: 6.7724

>NTDB_id=630969 LPH51_RS11255 WP_038272110.1 2689314..2690444(-) (pilU) [Xylella taiwanensis strain PLS427]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKMHGNITPITQVPLTSDQSRDMVFNVMTQAQREEFEKTHECNFAIDITN
IGRFRVNAFYQRSQIGMVLRRIEMSIPTFEKLTLPHVIKNLVMAKRGIIIIAGATGSGKSTSMASMVNYRNQNSTGHIIT
IEDPIEFMHKHENCIITQREIGTDTDSWENALKNTLRQAPDVIMIGEVRTQKTMDHAISFAETGHLVMCTLHANNANQAI
DRVINFFPEDRRGQLLMDLSLNLKGIIAQQLIQTPDGKGRCLAVEILLGTPLVQDYIRAGEIHKLKEIMQSSTNLGMKTF
DQSLVELYQAGAISHADALRYADSQNEVRLRIKLSEGKDAKTLEQGFDGIEIAEVS

Nucleotide

• Length: 1131 bp

>NTDB_id=630969 LPH51_RS11255 WP_038272110.1 2689314..2690444(-) (pilU) [Xylella taiwanensis strain PLS427]
ATGACGACCATCGATTTCACGTCCTTACTTAAGCTGATGGCGCACCAAAAGGCGTCAGACTTGTTTATCACATGTGGGAT
GCCACCATCGATGAAGATGCATGGCAATATCACTCCAATAACACAGGTACCGCTGACTTCAGACCAAAGCCGTGACATGG
TCTTCAATGTGATGACTCAGGCTCAGCGTGAAGAATTCGAGAAGACACACGAATGCAATTTCGCCATCGACATCACGAAC
ATTGGCCGCTTCCGCGTCAACGCATTCTATCAGCGCAGCCAGATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACGTTTGAGAAACTGACTCTCCCCCATGTGATCAAGAATCTCGTCATGGCAAAGCGTGGCATCATCATCATCGCTGGCG
CCACTGGCAGCGGCAAATCAACCTCAATGGCATCAATGGTCAACTACCGTAATCAAAACTCCACGGGGCATATCATCACT
ATCGAAGATCCCATTGAGTTCATGCACAAGCATGAGAACTGCATCATCACCCAACGCGAAATCGGTACCGATACCGATAG
TTGGGAAAACGCACTGAAAAACACTCTACGCCAGGCCCCAGATGTGATTATGATTGGTGAGGTACGTACCCAGAAAACGA
TGGACCACGCGATCTCCTTCGCTGAAACCGGCCATCTGGTAATGTGCACCTTGCATGCCAACAACGCCAATCAGGCAATA
GACCGTGTCATCAACTTCTTTCCGGAAGATCGGCGCGGTCAATTATTGATGGATCTGTCACTAAACCTAAAGGGCATCAT
CGCTCAACAATTAATCCAAACTCCGGATGGTAAAGGTCGCTGTTTAGCGGTGGAAATTTTGCTTGGCACTCCATTAGTCC
AAGACTACATTCGCGCAGGCGAGATCCACAAGCTCAAGGAAATCATGCAAAGCTCCACTAACCTTGGAATGAAGACTTTC
GATCAAAGCCTGGTAGAGCTGTATCAGGCCGGTGCGATTAGCCATGCAGATGCGCTACGCTATGCTGATTCGCAGAATGA
GGTACGCCTGCGCATTAAGCTCTCTGAGGGTAAAGACGCTAAAACCCTGGAGCAAGGATTCGATGGAATAGAGATTGCAG
AAGTGTCATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-272)

3D structure

• AlphaFold DB: Z9JI39

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.035	97.606	0.596
pilU	Acinetobacter baylyi ADP1	61.582	94.149	0.58
pilU	Vibrio cholerae strain A1552	52.247	94.681	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.408	94.415	0.391
pilT	Acinetobacter nosocomialis M2	39.826	91.489	0.364
pilT	Acinetobacter baumannii D1279779	39.826	91.489	0.364
pilT	Acinetobacter baumannii strain A118	39.826	91.489	0.364