Detailed information NTG0630824

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LPH41_RS00740
• Genome accession: NZ_CP087680
• Coordinates: 141635..142765 (+)
• Length: 376 a.a.
• NCBI ID: WP_038272110.1
• Uniprot ID: Z9JI39
• Organism: Xylella taiwanensis strain PLS407
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 136635..147765

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPH41_RS00730 (LPH41_00730)	-	137987..138838 (-)	852	WP_081755470.1	glycosyltransferase family 2 protein	-
LPH41_RS00735 (LPH41_00735)	pilT	140443..141477 (+)	1035	WP_038272165.1	type IV pilus twitching motility protein PilT	Machinery gene
LPH41_RS00740 (LPH41_00740)	pilU	141635..142765 (+)	1131	WP_038272110.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LPH41_RS00745 (LPH41_00745)	-	142996..143894 (-)	899	Protein_144	WYL domain-containing protein	-
LPH41_RS00750 (LPH41_00750)	-	145029..145838 (+)	810	WP_038272108.1	DUF481 domain-containing protein	-
LPH41_RS00755 (LPH41_00755)	hemC	145876..146793 (-)	918	WP_038272106.1	hydroxymethylbilane synthase	-
LPH41_RS00760 (LPH41_00760)	-	146801..147559 (-)	759	WP_038272104.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42121.51 Da
• Isoelectric Point: 6.7724

>NTDB_id=630824 LPH41_RS00740 WP_038272110.1 141635..142765(+) (pilU) [Xylella taiwanensis strain PLS407]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKMHGNITPITQVPLTSDQSRDMVFNVMTQAQREEFEKTHECNFAIDITN
IGRFRVNAFYQRSQIGMVLRRIEMSIPTFEKLTLPHVIKNLVMAKRGIIIIAGATGSGKSTSMASMVNYRNQNSTGHIIT
IEDPIEFMHKHENCIITQREIGTDTDSWENALKNTLRQAPDVIMIGEVRTQKTMDHAISFAETGHLVMCTLHANNANQAI
DRVINFFPEDRRGQLLMDLSLNLKGIIAQQLIQTPDGKGRCLAVEILLGTPLVQDYIRAGEIHKLKEIMQSSTNLGMKTF
DQSLVELYQAGAISHADALRYADSQNEVRLRIKLSEGKDAKTLEQGFDGIEIAEVS

Nucleotide

• Length: 1131 bp

>NTDB_id=630824 LPH41_RS00740 WP_038272110.1 141635..142765(+) (pilU) [Xylella taiwanensis strain PLS407]
ATGACGACCATCGATTTCACGTCCTTACTTAAGCTGATGGCGCACCAAAAGGCGTCAGACTTGTTTATCACATGTGGGAT
GCCACCATCGATGAAGATGCATGGCAATATCACTCCAATAACACAGGTACCGCTGACTTCAGACCAAAGCCGTGACATGG
TCTTCAATGTGATGACTCAGGCTCAGCGTGAAGAATTCGAGAAGACACACGAATGCAATTTCGCCATCGACATCACGAAC
ATTGGCCGCTTCCGCGTCAACGCATTCTATCAGCGCAGCCAGATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACGTTTGAGAAACTGACTCTCCCCCATGTGATCAAGAATCTCGTCATGGCAAAGCGTGGCATCATCATCATCGCTGGCG
CCACTGGCAGCGGCAAATCAACCTCAATGGCATCAATGGTCAACTACCGTAATCAAAACTCCACGGGGCATATCATCACT
ATCGAAGATCCCATTGAGTTCATGCACAAGCATGAGAACTGCATCATCACCCAACGCGAAATCGGTACCGATACCGATAG
TTGGGAAAACGCACTGAAAAACACTCTACGCCAGGCCCCAGATGTGATTATGATTGGTGAGGTACGTACCCAGAAAACGA
TGGACCACGCGATCTCCTTCGCTGAAACCGGCCATCTGGTAATGTGCACCTTGCATGCCAACAACGCCAATCAGGCAATA
GACCGTGTCATCAACTTCTTTCCGGAAGATCGGCGCGGTCAATTATTGATGGATCTGTCACTAAACCTAAAGGGCATCAT
CGCTCAACAATTAATCCAAACTCCGGATGGTAAAGGTCGCTGTTTAGCGGTGGAAATTTTGCTTGGCACTCCATTAGTCC
AAGACTACATTCGCGCAGGCGAGATCCACAAGCTCAAGGAAATCATGCAAAGCTCCACTAACCTTGGAATGAAGACTTTC
GATCAAAGCCTGGTAGAGCTGTATCAGGCCGGTGCGATTAGCCATGCAGATGCGCTACGCTATGCTGATTCGCAGAATGA
GGTACGCCTGCGCATTAAGCTCTCTGAGGGTAAAGACGCTAAAACCCTGGAGCAAGGATTCGATGGAATAGAGATTGCAG
AAGTGTCATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-272)

3D structure

• AlphaFold DB: Z9JI39

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.035	97.606	0.596
pilU	Acinetobacter baylyi ADP1	61.582	94.149	0.58
pilU	Vibrio cholerae strain A1552	52.247	94.681	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.408	94.415	0.391
pilT	Acinetobacter nosocomialis M2	39.826	91.489	0.364
pilT	Acinetobacter baumannii D1279779	39.826	91.489	0.364
pilT	Acinetobacter baumannii strain A118	39.826	91.489	0.364