Detailed information NTG0630367

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LO769_RS08910
• Genome accession: NZ_CP087600
• Coordinates: 1787481..1788128 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis subsp. lactis strain IBB109
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1782481..1793128

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LO769_RS08885 (LO769_08880)	atpF	1782551..1783057 (-)	507	WP_004255258.1	F0F1 ATP synthase subunit B	-
LO769_RS08890 (LO769_08885)	atpB	1783072..1783785 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LO769_RS08895 (LO769_08890)	-	1783830..1784045 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LO769_RS08900 (LO769_08895)	-	1784229..1785005 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LO769_RS08905 (LO769_08900)	comEC	1785290..1787500 (-)	2211	WP_252172302.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LO769_RS08910 (LO769_08905)	comEA	1787481..1788128 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
LO769_RS08915 (LO769_08910)	-	1788188..1789549 (-)	1362	WP_021214597.1	ABC transporter permease	-
LO769_RS08920 (LO769_08915)	-	1789546..1790478 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LO769_RS08925 (LO769_08920)	-	1790583..1790981 (-)	399	WP_004255236.1	hypothetical protein	-
LO769_RS08930 (LO769_08925)	-	1791085..1791648 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LO769_RS08935 (LO769_08930)	-	1791821..1792999 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=630367 LO769_RS08910 WP_004255240.1 1787481..1788128(-) (comEA) [Lactococcus lactis subsp. lactis strain IBB109]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=630367 LO769_RS08910 WP_004255240.1 1787481..1788128(-) (comEA) [Lactococcus lactis subsp. lactis strain IBB109]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367