Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY68_RS02090 Genome accession   NZ_CP087205
Coordinates   468250..470337 (+) Length   695 a.a.
NCBI ID   WP_163907876.1    Uniprot ID   A0A6L5BYQ9
Organism   Pseudomonas sp. B21-053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463250..475337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY68_RS02070 (LOY68_02065) comM 465462..466526 (+) 1065 WP_163907870.1 pilus assembly protein PilM Machinery gene
  LOY68_RS02075 (LOY68_02070) - 466526..467092 (+) 567 WP_265042631.1 PilN domain-containing protein -
  LOY68_RS02080 (LOY68_02075) pilO 467089..467712 (+) 624 WP_163907874.1 type 4a pilus biogenesis protein PilO -
  LOY68_RS02085 (LOY68_02080) - 467709..468236 (+) 528 WP_265042632.1 pilus assembly protein PilP -
  LOY68_RS02090 (LOY68_02085) pilQ 468250..470337 (+) 2088 WP_163907876.1 type IV pilus secretin PilQ Machinery gene
  LOY68_RS02095 (LOY68_02090) aroK 470342..470860 (+) 519 WP_265042634.1 shikimate kinase AroK -
  LOY68_RS02100 (LOY68_02095) aroB 471012..472112 (+) 1101 WP_265042635.1 3-dehydroquinate synthase -
  LOY68_RS02105 (LOY68_02100) - 472123..473712 (+) 1590 WP_265042636.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75185.59 Da        Isoelectric Point: 5.8321

>NTDB_id=628113 LOY68_RS02090 WP_163907876.1 468250..470337(+) (pilQ) [Pseudomonas sp. B21-053]
MNRIFSTLGISLWIALLSPMVQAATLNALDVAALPGDRVELKLTFDGPPPPPHGYTTESPARIALDLPGVVSKLANKNRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYSSRVEGNNLFVVVGQGAKSTAAKPVASAPRPATAKPAPAKAYAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILGFARTQLPDPLRVRLDVKDFATPVQFVNASATGDRAVISIEPSG
AFEYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFTSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGANASNTIGTPGSTSTNSPFVDMGTAANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=628113 LOY68_RS02090 WP_163907876.1 468250..470337(+) (pilQ) [Pseudomonas sp. B21-053]
ATGAACAGGATTTTCTCAACCCTCGGTATTTCGCTATGGATAGCGCTGCTGTCGCCGATGGTACAAGCGGCCACCCTCAA
CGCGTTGGATGTCGCCGCGCTGCCGGGTGATCGCGTCGAGTTGAAGTTGACCTTCGATGGGCCGCCACCTCCGCCCCACG
GCTACACCACCGAATCACCGGCGCGCATCGCGCTGGATCTGCCGGGCGTGGTCAGCAAACTGGCCAACAAAAACCGTGAC
CTCGGTGGCGGCAATGCCCGCAGCGCCACGGTGGTCGAAGCCAAGGACCGCACGCGGCTGATCATCAACCTGACTCAACT
GACGCCCTATAGCTCCAGGGTTGAGGGCAACAATCTGTTCGTGGTCGTTGGGCAAGGCGCGAAAAGCACGGCGGCCAAAC
CCGTCGCCAGCGCACCCCGCCCAGCTACCGCAAAACCGGCGCCCGCCAAGGCCTATGCGCCCGTCGGCAAGGCCATTCGC
GGCGTGGATTTTCAGCGCGGCACCCAAGGTGAAGGCAATGTGGTGATCGACTTGTCGGATCCATCCATTGCACCGGATAT
TCAGGAGCGCGAGGGCAAGATCATCCTCGGTTTCGCCAGGACCCAATTGCCTGACCCGTTACGGGTGCGCCTGGACGTCA
AGGATTTCGCGACTCCGGTGCAGTTCGTCAACGCCAGTGCCACCGGTGACCGCGCGGTGATCAGCATCGAGCCGAGCGGC
GCTTTCGAATACTCGACCTACCAGACCGACAACAAGCTGACCGTCAGCATCCGTCCGATGACCGTCGATGACTTGCAGAA
GCGCAACGCCGAGCGCTTTGCCTACACCGGCGAAAAGCTCTCGCTGAACTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAACCTCGTGGCCAGTGACACGGTTCAGGGCGGCATCACCTTGCGTCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGATCGGCAACGTCTTGCTGGT
CGCGCCAGCGGATGAAATCGCCGCCCGTGAGCGTCAGGAACTGGAGTCGCAAAAACAGATCGCCGAACTGGCGCCGCTGC
GGCGGGAGCTGTTGCAGGTCAATTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCACTTCGGTGACCAGTGCCGAGGCG
AAAATCGATGAGCGGGGTTCGATCACGGTCGATGAGCGCACCAACAACATCATCGCCTACCAGACCCAGGATCGCCTCGA
CGAGCTTCGCCGCATCGTTGCGCAACTGGATATCCCGGTGCGCCAGGTGATGATCGAAGCGCGGATCGTCGAAGCCAACG
TCGATTACGACAAGAGTCTCGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACACCTCGGGTGTCAGT
AATGGCGCCAATGCTTCGAACACCATCGGCACGCCCGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGC
GGCTAACACCTCGGGGCTCGGCATCGCCTTCATCACCGACAATGTGCTGCTGGATCTTGAGCTGACGGCCATGGAAAAAA
CCGGCAACGGCGAAATCGTCTCGCAGCCCAAAGTGGTCACGTCCGATAAGGAAACCGCGAAAATCCTCAAGGGCACCGAG
ATTCCGTATCAGGAGGCCAGCTCCAGTGGTGCGACGTCGGTGTCGTTCAAAGAGGCTTCGCTGTCCCTGGAAGTGACGCC
GCAAATCACCCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTCAACAAAGTGCAGG
ATGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTTAACGACGGCGAGACCATCGTCATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGACGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6L5BYQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.986

100

0.761
  comQ Acinetobacter baylyi ADP1

42.429

100

0.427
  pilQ Acinetobacter baumannii D1279779

37.903

100

0.406
  pilQ Acinetobacter baumannii strain A118

37.903

100

0.406
  pilQ Legionella pneumophila strain Lp02

37.739

97.986

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.739

97.986

0.37