Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   LOY68_RS02070 Genome accession   NZ_CP087205
Coordinates   465462..466526 (+) Length   354 a.a.
NCBI ID   WP_163907870.1    Uniprot ID   A0A6L5BVK1
Organism   Pseudomonas sp. B21-053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 460462..471526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY68_RS02060 (LOY68_02055) - 461334..462602 (+) 1269 WP_163907866.1 malic enzyme-like NAD(P)-binding protein -
  LOY68_RS02065 (LOY68_02060) - 462802..465243 (-) 2442 WP_413787185.1 penicillin-binding protein 1A -
  LOY68_RS02070 (LOY68_02065) comM 465462..466526 (+) 1065 WP_163907870.1 pilus assembly protein PilM Machinery gene
  LOY68_RS02075 (LOY68_02070) - 466526..467092 (+) 567 WP_265042631.1 PilN domain-containing protein -
  LOY68_RS02080 (LOY68_02075) pilO 467089..467712 (+) 624 WP_163907874.1 type 4a pilus biogenesis protein PilO -
  LOY68_RS02085 (LOY68_02080) - 467709..468236 (+) 528 WP_265042632.1 pilus assembly protein PilP -
  LOY68_RS02090 (LOY68_02085) pilQ 468250..470337 (+) 2088 WP_163907876.1 type IV pilus secretin PilQ Machinery gene
  LOY68_RS02095 (LOY68_02090) aroK 470342..470860 (+) 519 WP_265042634.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38173.68 Da        Isoelectric Point: 4.8199

>NTDB_id=628112 LOY68_RS02070 WP_163907870.1 465462..466526(+) (comM) [Pseudomonas sp. B21-053]
MLGLFNKKTRTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVIEKNIAELEGVGQALSRVLLKARTGIRNVAV
AVAGSAVITKTIEMDAGLSDEEMENQLKIEADQYIPYPLDEVAIDFEVQGVSVRNPERVNVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLATQLASNQERPTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTT
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=628112 LOY68_RS02070 WP_163907870.1 465462..466526(+) (comM) [Pseudomonas sp. B21-053]
GTGCTAGGACTCTTCAATAAAAAGACCAGAACGCTTCTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTTCTGGAGCT
CAGCCGCCAGGGCGACCGATACCGGGTCGAGGCCTACGCGGTCGAACCGCTGCCGGCCAGCGCGGTCATCGAGAAGAACA
TCGCCGAGCTTGAAGGTGTGGGCCAGGCGTTGTCACGCGTGCTTCTCAAGGCTCGGACCGGTATCCGGAACGTCGCGGTT
GCGGTGGCCGGTTCGGCGGTGATTACCAAGACCATCGAGATGGACGCCGGGCTGTCCGACGAAGAGATGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCTTATCCATTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGTGTATCGGTGC
GCAACCCCGAGCGGGTCAATGTGCTGCTGGCCGCCTGTCGGAAAGAAAACGTCGAAGTTCGCGAGGCGGCGCTGGCTCTC
GCCGGGCTGACCGCCAAGGTGGTGGACGTCGAAGCTTATGCCCTCGAACGTTCCTTTGGCCTGCTGGCGACCCAACTGGC
CTCCAATCAAGAACGCCCGACCGTCGCGGTGGTGGACATCGGCGCGACCATGACCACCCTGAGCGTGCTGCATAACGGCC
GGATCATCTACACCCGCGAACAATTGTTCGGCGGTCGCCAACTCACTGAAGAAATCCAGCGCCGCTATGGCCTGACCACT
GAGCAGGCCGGGCTGGCAAAGAAGCAGGGCGGTTTGCCGGACGACTATGTCAGCGAGGTGTTGCAGCCGTTTCGCGATGC
GCTGGTGCAGCAGGTCTCTCGCTCATTGCAGTTTTTCTTTGCGTCCGGGCAGTACAACGCGGTCGACCATATCCTGCTGG
CCGGCGGCACCGCCTCGGTGCCCGGGCTTGATCGATTGATCGAGCAGCGGCTGGGCACGCCGACCCAGGTCGCCAACCCA
TTCGCGGACATGGCGCTGAGCAGCAAGGTCAACGCCGGCGCCCTCGCCAGCGATGCCCCGGCCTTGATGATTGCCTGCGG
CCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6L5BVK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486