Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY70_RS02115 Genome accession   NZ_CP087204
Coordinates   475792..477867 (+) Length   691 a.a.
NCBI ID   WP_265032909.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-054     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470792..482867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY70_RS02095 (LOY70_02090) pilM 473003..474067 (+) 1065 WP_123414962.1 pilus assembly protein PilM Machinery gene
  LOY70_RS02100 (LOY70_02095) - 474067..474633 (+) 567 WP_265032905.1 PilN domain-containing protein -
  LOY70_RS02105 (LOY70_02100) pilO 474630..475253 (+) 624 WP_025211415.1 type 4a pilus biogenesis protein PilO -
  LOY70_RS02110 (LOY70_02105) - 475250..475795 (+) 546 WP_265032907.1 pilus assembly protein PilP -
  LOY70_RS02115 (LOY70_02110) pilQ 475792..477867 (+) 2076 WP_265032909.1 type IV pilus secretin PilQ Machinery gene
  LOY70_RS02120 (LOY70_02115) aroK 477872..478390 (+) 519 WP_123345370.1 shikimate kinase AroK -
  LOY70_RS02125 (LOY70_02120) aroB 478520..479620 (+) 1101 WP_123345367.1 3-dehydroquinate synthase -
  LOY70_RS02130 (LOY70_02125) - 479631..481211 (+) 1581 WP_265032913.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74744.91 Da        Isoelectric Point: 5.5772

>NTDB_id=628083 LOY70_RS02115 WP_265032909.1 475792..477867(+) (pilQ) [Pseudomonas sp. B21-054]
MNRFLSAFGISLWIALLSPMVHAASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTDQPARIALDLPGVTNQLTSKSRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYNTRVEGNNLFVVVGKGVVVPKATAQAARPAVASTAPARGNAPVGKAIRGV
DFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGGDRATITIEPSGTF
DYSTYQTDNKLTVSVRPMTVEDLQKRNTDRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKA
DERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSGVTNG
TSTTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=628083 LOY70_RS02115 WP_265032909.1 475792..477867(+) (pilQ) [Pseudomonas sp. B21-054]
ATGAACAGGTTTTTGTCAGCCTTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCACGCGGCCAGTCTGAA
AACGCTGGATGTCGCGGCGTTGCCGGGCGACCGCGTCGAGTTGAAGCTCACCTTCGACGGGCCGCCGCCCACGCCCCGGG
GCTACACCACCGATCAACCGGCCCGGATCGCACTGGATCTGCCCGGGGTCACCAATCAACTGACCAGCAAGAGCCGCGAC
CTGGGGGGCGGCAATGCGCGCAGCGCCACAGTGGTCGAGGCGGGCGACCGCACGCGGTTGATCATCGGCCTTACCCAGCT
GGCGCCGTATAACACCCGTGTCGAGGGTAATAATCTGTTCGTGGTGGTTGGCAAAGGCGTCGTAGTGCCCAAGGCCACCG
CCCAGGCTGCGCGCCCAGCGGTTGCCAGCACCGCGCCCGCCAGAGGCAATGCCCCGGTGGGCAAGGCTATTCGTGGCGTG
GATTTCCAGCGCGGGACCCAGGGTGAAGGCAATGTGGTGATCGATCTGTCGGACCCTTCCATCGCCCCGGACATCCAGGA
ACGCGAAGGCAAGATCATTCTCAATTTCGCCAGGACCCAGTTGCCCGAACCGCTGCGGGTGCGCCTGGACGTCAAGGACT
TCGCCACCCCGGTGCAATTCGTCAATGCCAGTGCGGGGGGCGACCGAGCGACGATCACCATCGAGCCGAGCGGCACCTTC
GATTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGAGGATCTGCAGAAACGCAA
TACCGATCGCGCCGCCTACAGTGGTGAAAAGCTGTCCTTGAACTTCCAGGACATCGAAGTGCGTTCTGTCCTGCAGTTGA
TCGCCGACTTCACCAACCTGAACCTGGTGGCCAGCGACACCGTGCAGGGCGGCATCACCCTGCGCCTGCAGAACGTGCCT
TGGGATCAGGCGCTGGATCTCGTGCTGAAGACCAAAGGACTGGACAAGCGCAAGGTTGGCAACGTGCTGCTGGTCGCCCC
GGCCGATGAAATTGCCGCGCGCGAGCGCCAGGAACTGGAGTCCCAGAAGCAGATTGCCGACCTGGCGCCGCTGCGGCGTG
AGTTGTTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAAGCGAAAGCC
GATGAGCGCGGCACGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTCGACGAACT
GCGCCGGATCGTGGCGCAGCTGGATATTCCAGTGCGTCAGGTGATGATCGAGGCGCGAATCGTCGAGGCCAACGTCGACT
ACGACAAAAGCCTGGGTGTGCGCTGGGGCGGTTCGATACAAAACAAGGGCAACTGGAACTCCTCGGGGGTGACCAATGGC
ACCTCCACGACCATTGGCACGCCGGGCAGCACCAGTACCAACCAGCCGTTCGTCGATCTGGGCGCGTCGGGCAATACTTC
CGGGATCGGTATCGCGTTCATTACCGACAACGTCTTGCTGGACCTGGAGCTGACGGCCATGGAGAAAACCGGTAACGGGG
AAATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAAATCCTCAAGGGCACGGAAATCCCCTATCAG
GAAGCCAGCTCCAGCGGCGCCACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTGACCCCGCAGATCACACC
GGACAATCGCATCATCATGGAGGTCAAGGTTACCAAGGATGAGCCGGACTACCTGAACAAAGTGCAGGATGTGCCACCGA
TCAAGAAAAACGAAGTCAACGCCAAGGTCCTGGTCAACGATGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Secretin and TonB N terminus short domain (STN)

(301-347)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(526-682)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.287

100

0.758
  pilQ Acinetobacter baumannii strain A118

38.792

100

0.418
  pilQ Acinetobacter baumannii D1279779

38.792

100

0.418
  comQ Acinetobacter baylyi ADP1

41.618

100

0.417
  pilQ Legionella pneumophila strain Lp02

39.111

97.685

0.382
  pilQ Legionella pneumophila strain ERS1305867

38.988

97.25

0.379