Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY70_RS02095 Genome accession   NZ_CP087204
Coordinates   473003..474067 (+) Length   354 a.a.
NCBI ID   WP_123414962.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-054     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468003..479067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY70_RS02085 (LOY70_02080) - 468835..470103 (+) 1269 WP_003186625.1 malic enzyme-like NAD(P)-binding protein -
  LOY70_RS02090 (LOY70_02085) - 470350..472794 (-) 2445 WP_405045698.1 penicillin-binding protein 1A -
  LOY70_RS02095 (LOY70_02090) pilM 473003..474067 (+) 1065 WP_123414962.1 pilus assembly protein PilM Machinery gene
  LOY70_RS02100 (LOY70_02095) - 474067..474633 (+) 567 WP_265032905.1 PilN domain-containing protein -
  LOY70_RS02105 (LOY70_02100) pilO 474630..475253 (+) 624 WP_025211415.1 type 4a pilus biogenesis protein PilO -
  LOY70_RS02110 (LOY70_02105) - 475250..475795 (+) 546 WP_265032907.1 pilus assembly protein PilP -
  LOY70_RS02115 (LOY70_02110) pilQ 475792..477867 (+) 2076 WP_265032909.1 type IV pilus secretin PilQ Machinery gene
  LOY70_RS02120 (LOY70_02115) aroK 477872..478390 (+) 519 WP_123345370.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38176.70 Da        Isoelectric Point: 4.9070

>NTDB_id=628082 LOY70_RS02095 WP_123414962.1 473003..474067(+) (pilM) [Pseudomonas sp. B21-054]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVIEKNIAELEGVGQALSRVLAKAKTASRSVAV
AVAGSAVITKIIEMDAGMSDDDMENQLKIEADQYIPYPLDEVAIDFEVLGVSPRSTERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGQAKRQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=628082 LOY70_RS02095 WP_123414962.1 473003..474067(+) (pilM) [Pseudomonas sp. B21-054]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTCTGGGAATAGACATCAGCTCCACCTCGGTGAAGCTGCTTGAGTT
GAGCCGCCAGGGTGACCGGTACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAACGCCGTGATCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGCCAGGCATTGTCTCGGGTGCTCGCCAAGGCCAAGACCGCCTCGCGAAGCGTGGCAGTG
GCCGTGGCGGGTTCGGCGGTGATCACCAAGATCATCGAGATGGACGCCGGGATGTCCGACGACGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAGTACATTCCTTATCCGCTGGATGAGGTCGCCATCGATTTTGAAGTGCTGGGCGTGTCACCGC
GCAGCACCGAGCGGGTCGAGGTGTTGTTGGCGGCCTGTCGCAAGGAAAACGTCGAGGTTCGCGAGGCTGCGCTGGCGCTG
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCCTACGCGCTGGAGCGCGCCTTTGGCCTGCTCGCCACGCAACTGGC
GGCGTCCCAGGAGCGGCTGACTGTGGCGGTCATCGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GGATCATCTATACCCGCGAGCAGTTGTTCGGCGGCCGTCAGCTCACCGAGGAAATCCAGCGCCGCTATGGCCTGACGCCC
GAGCAGGCCGGCCAGGCAAAAAGGCAGGGTGGCCTGCCGGACGATTATCTCAGTGAGGTGCTGCAACCCTTTCGCGAGGC
CCTGGTGCAGCAGGTTTCGCGGTCCTTGCAGTTTTTCTTCGCTTCGGGCCAGTACAGCGCGGTGGACCACATTTTGTTGG
CCGGAGGCACGGCGTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAACGCCTGGGCACACCGACCCAGGTCGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGTGCCCTGGCCAGTGACGCGCCGGCGCTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475