Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY67_RS02065 Genome accession   NZ_CP087203
Coordinates   463858..465930 (+) Length   690 a.a.
NCBI ID   WP_265065725.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-056     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458858..470930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY67_RS02045 (LOY67_02045) comM 461070..462134 (+) 1065 WP_265065721.1 pilus assembly protein PilM Machinery gene
  LOY67_RS02050 (LOY67_02050) - 462134..462700 (+) 567 WP_265065722.1 PilN domain-containing protein -
  LOY67_RS02055 (LOY67_02055) pilO 462697..463320 (+) 624 WP_265065723.1 type 4a pilus biogenesis protein PilO -
  LOY67_RS02060 (LOY67_02060) - 463317..463844 (+) 528 WP_265065724.1 pilus assembly protein PilP -
  LOY67_RS02065 (LOY67_02065) pilQ 463858..465930 (+) 2073 WP_265065725.1 type IV pilus secretin PilQ Machinery gene
  LOY67_RS02070 (LOY67_02070) aroK 465935..466453 (+) 519 WP_265065726.1 shikimate kinase AroK -
  LOY67_RS02075 (LOY67_02075) aroB 466602..467702 (+) 1101 WP_265065727.1 3-dehydroquinate synthase -
  LOY67_RS02080 (LOY67_02080) - 467713..469284 (+) 1572 WP_265065728.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74742.94 Da        Isoelectric Point: 5.2033

>NTDB_id=628054 LOY67_RS02065 WP_265065725.1 463858..465930(+) (pilQ) [Pseudomonas sp. B21-056]
MNRILTALGVSLWIALMSPMVLGASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLARKTQD
LGSGNARSATVVEAGDRTRLIIGLTQLVPYNARVEGNNLFVVVGKGAEPKTAAQAPRPAVAGAMPARSNAPAGRAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEPGGTFD
YSTYQTDNKLTVSVRPMTVEDLRKRDTDRAAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNQGNWNSSGVTNGS
STTIGTPGSTSTNQPFVDMGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDLVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=628054 LOY67_RS02065 WP_265065725.1 463858..465930(+) (pilQ) [Pseudomonas sp. B21-056]
ATGAACAGGATTCTTACCGCCCTCGGTGTTTCGCTATGGATAGCGCTGATGTCACCGATGGTCCTTGGGGCGAGCCTGAA
GACCCTGGATGTCGCGGCGCTGCCGGGGGACCGTGTCGAGTTGAAGCTCACGTTCGACGGACCACCGCCAACGCCCCGTG
GCTATACCACCGAACAGCCGGCCCGGATTGCCCTGGACCTGCCCGGGGTGACCAACCAGCTGGCGCGCAAGACCCAGGAC
CTTGGCAGCGGCAATGCACGCAGCGCCACCGTGGTCGAGGCGGGGGACCGCACCCGGCTGATCATCGGTCTTACCCAGCT
GGTGCCGTATAACGCCCGGGTCGAGGGCAATAACCTGTTCGTGGTGGTCGGCAAGGGCGCGGAACCGAAAACCGCCGCCC
AGGCCCCACGCCCGGCGGTTGCCGGTGCGATGCCGGCCAGGAGTAACGCACCGGCGGGCCGGGCCATCCGTGGCGTGGAC
TTCCAGCGCGGGACCCAGGGCGAAGGCAATGTCGTGATCGACCTGTCGGACCCGTCCATTGCGCCGGATATCCAGGAGCG
CGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAATTGCCTGAACCGCTGCGGGTGCGCCTGGACGTCAAGGACTTCG
CCACCCCGGTACAGTTCGTCAATGCCAGCGCCGGCTCCGACCGGGCCACCATCAGCATCGAGCCCGGCGGTACCTTCGAT
TACTCGACCTACCAGACCGACAACAAGCTGACCGTCAGTGTGAGGCCCATGACTGTAGAGGACCTGCGCAAGCGCGACAC
CGACCGTGCCGCCTACAACGGTGAGAAGCTGTCCTTGAATTTCCAGGACATCGAGGTGCGCTCGGTCCTGCAACTGATTG
CCGACTTCACCAACCTGAACCTGGTGGCAAGCGACACGGTGCAGGGCGGTATCACCCTGCGGTTGCAGAATGTTCCATGG
GACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAACGTCTTGCTGGTGGCCCCGGC
CGATGAGATTGCCGCTCGCGAGCGCCAGGAACTGGAGTCCCAGAAGCAGATTGCCGACCTGGCCCCGCTGCGACGGGAGC
TGTTGCAGGTCAACTATGCGAAGGCGGCGGACATTGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCGAAGGCCGAT
GAGCGCGGCACGATCACGGTGGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTCGACGAATTGCG
CCGGATCGTGGCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCCCGGATCGTCGAGGCCAACGTCGATTACG
ACAAGAGCCTGGGTGTGCGTTGGGGCGGCTCGGTGCAGAACCAGGGCAACTGGAATTCTTCCGGTGTGACCAACGGCAGC
TCCACCACCATCGGCACGCCAGGCAGCACCAGCACCAACCAGCCGTTCGTCGACATGGGCGCGTCGGGCAATACCTCGGG
CATCGGCATTGCGTTCATCACCGACAACGTATTGCTGGACCTGGAGCTGACGGCCATGGAGAAGACCGGCAACGGCGAAA
TCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACCGCAAAAATCCTCAAGGGCACGGAGATCCCCTATCAGGAA
GCCAGTTCCAGCGGTGCCACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTGACCCCGCAGATCACCCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTGCAGGAAGTACCACCGATCA
AGAAGAACGAAGTCAACGCCAAGGTGCTGGTCAATGACGGCGAGACCATTGTGATTGGCGGCGTTTTCTCAAATACTCAG
AGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATCTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.727

100

0.754
  comQ Acinetobacter baylyi ADP1

41.534

100

0.416
  pilQ Acinetobacter baumannii strain A118

38.243

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.243

100

0.41
  pilQ Legionella pneumophila strain ERS1305867

38.839

97.391

0.378
  pilQ Legionella pneumophila strain Lp02

38.599

97.246

0.375