Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY63_RS02025 Genome accession   NZ_CP087202
Coordinates   449955..452030 (+) Length   691 a.a.
NCBI ID   WP_010453646.1    Uniprot ID   -
Organism   Pseudomonas asplenii strain B21-058     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444955..457030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY63_RS02005 (LOY63_02005) pilM 447169..448233 (+) 1065 WP_010453641.1 pilus assembly protein PilM Machinery gene
  LOY63_RS02010 (LOY63_02010) - 448233..448799 (+) 567 WP_010453642.1 PilN domain-containing protein -
  LOY63_RS02015 (LOY63_02015) pilO 448796..449419 (+) 624 WP_010453643.1 type 4a pilus biogenesis protein PilO -
  LOY63_RS02020 (LOY63_02020) pilP 449416..449943 (+) 528 WP_010453644.1 type 4a pilus biogenesis lipoprotein PilP -
  LOY63_RS02025 (LOY63_02025) pilQ 449955..452030 (+) 2076 WP_010453646.1 type IV pilus secretin PilQ Machinery gene
  LOY63_RS02030 (LOY63_02030) aroK 452035..452553 (+) 519 WP_010453648.1 shikimate kinase AroK -
  LOY63_RS02035 (LOY63_02035) aroB 452703..453803 (+) 1101 WP_010453651.1 3-dehydroquinate synthase -
  LOY63_RS02040 (LOY63_02040) - 453814..455397 (+) 1584 WP_010453652.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74773.98 Da        Isoelectric Point: 5.4228

>NTDB_id=628024 LOY63_RS02025 WP_010453646.1 449955..452030(+) (pilQ) [Pseudomonas asplenii strain B21-058]
MNRILSALGISLWMALVSPMVFAANLKTLDVAALPGDRVEIKLAFDSPPAAPHGYTTEQPARIALDLTGVASQLATKTRD
LGVGNARSVTVVEAKDRTRLIINLTKLAPYSTRIEGNNLFVVVGQGAAPAVATPLASTRPVATAAQSARVAPKPYVAVGK
AIRSVDFQRGEQGEGNVVIELSDPSISPDVQEKGGKISVEFPRTQLPESLRVRLDVKDFATPVQYVNATASADRASISIE
PSGAFDYSTYQTDNKLTISVRPLTVDDLQRRNAERFVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AESKGDERGSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVGYDKQLGARWGGSISGGNWKTNG
GSPTLSRLTGQSASTPFVDLGATSATSGIGIGFVTDNTILDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGTEIPYQ
ESSSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYQNVLNNVPPIKKNEVNAKVLVNDGETVVIGGVFSNT
QSKTEEKVPFLGDVPYVGRLFRRDIVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=628024 LOY63_RS02025 WP_010453646.1 449955..452030(+) (pilQ) [Pseudomonas asplenii strain B21-058]
ATGAACAGGATCCTTTCCGCCCTCGGCATTTCGCTATGGATGGCGCTGGTGTCGCCGATGGTGTTCGCGGCCAATCTCAA
GACCCTCGACGTGGCGGCACTGCCGGGCGATCGGGTCGAGATCAAGCTGGCTTTCGACAGCCCGCCTGCGGCCCCGCACG
GCTATACCACCGAGCAGCCGGCGCGGATTGCGCTGGACCTGACCGGAGTGGCCAGCCAACTGGCTACCAAGACTCGCGAC
CTGGGTGTCGGTAACGCGCGTAGCGTGACCGTGGTAGAGGCCAAGGATCGGACCCGGCTGATCATCAACCTGACCAAGCT
GGCGCCCTACAGCACGCGGATCGAGGGCAACAACCTGTTCGTGGTGGTCGGTCAGGGCGCTGCACCCGCTGTTGCGACTC
CGCTCGCCAGTACCCGGCCTGTAGCTACCGCTGCGCAGTCGGCGCGTGTGGCACCCAAACCCTACGTGGCTGTTGGCAAG
GCCATTCGCAGCGTTGACTTCCAGCGCGGCGAGCAGGGCGAAGGCAATGTGGTGATCGAGCTTTCCGATCCCTCCATCAG
TCCGGATGTGCAGGAAAAGGGCGGCAAGATCAGCGTGGAGTTTCCGCGTACCCAACTGCCGGAATCCCTGCGCGTGCGCC
TGGATGTGAAGGATTTCGCCACGCCGGTGCAATACGTCAACGCCACGGCGTCTGCCGACCGGGCAAGCATCTCCATCGAG
CCGAGTGGTGCCTTCGATTATTCGACCTACCAGACCGACAATAAACTGACCATCAGTGTCCGTCCGCTGACCGTCGATGA
CCTGCAGCGACGTAACGCCGAGCGCTTTGTCTACACCGGTGAAAAACTCTCGCTGAACTTCCAGGACATCGATGTGCGTT
CGGTTCTGCAACTGATCGCCGATTTTACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAAGGCGGGATCACCCTGCGC
CTGCAAAACGTGCCGTGGGACCAGGCATTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGATCGGCAACGT
GCTGTTGGTGGCGCCGGCGGACGAGATCGCTGCGCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCAGAGCTTG
CGCCACTGCGTCGTGAGCTGCTGCAGGTCAACTACGCCAAGGCCGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGC
GCCGAGAGCAAGGGCGACGAGCGTGGCTCGATCACCGTGGACGAACGCACCAACAACATCATTGCCTACCAGACCCAGGA
GCGTCTCGACGAACTGCGACGGATCGTTGCGCAACTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGTATCGTCG
AGGCCAACGTCGGTTATGACAAACAATTGGGGGCCCGTTGGGGCGGCAGTATCTCGGGTGGCAACTGGAAAACCAATGGC
GGCTCGCCAACGTTGTCGAGGTTGACTGGCCAGAGTGCCAGTACACCGTTCGTCGATCTGGGAGCAACCTCGGCGACCTC
CGGCATCGGTATCGGCTTCGTCACCGACAACACCATCCTCGACTTGGAACTGAGTGCGATGGAGAAGACCGGTAACGGTG
AGGTCGTGTCGCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACCGAGATCCCGTACCAG
GAGTCCAGCTCCAGCGGTGCCACTTCGGTGTCCTTCAAGGAGGCGTCGCTGTCGCTTGAGGTCACGCCGCAGATCACCCC
GGACAATCGGATCATCATGGAGGTGAAGGTCACCAAGGATGAGCCGGACTACCAGAACGTGCTCAACAACGTACCGCCGA
TCAAGAAAAACGAAGTCAACGCCAAGGTGCTGGTCAATGACGGTGAAACCGTGGTGATTGGCGGTGTGTTCTCCAATACT
CAGAGCAAAACCGAAGAGAAGGTGCCATTTCTCGGCGATGTGCCGTATGTTGGGCGCCTTTTCCGTCGGGACATCGTTTC
GGAAGCAAAATCCGAGCTGCTGGTATTCCTGACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

Bacterial type II and III secretion system protein (Secretin)

(526-682)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.059

100

0.768
  comQ Acinetobacter baylyi ADP1

42.017

100

0.434
  pilQ Acinetobacter baumannii strain A118

39.001

100

0.418
  pilQ Acinetobacter baumannii D1279779

39.001

100

0.418
  pilQ Legionella pneumophila strain Lp02

39.201

97.829

0.384
  pilQ Legionella pneumophila strain ERS1305867

39.201

97.829

0.384