Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY63_RS02005 Genome accession   NZ_CP087202
Coordinates   447169..448233 (+) Length   354 a.a.
NCBI ID   WP_010453641.1    Uniprot ID   A0A1H6LC79
Organism   Pseudomonas asplenii strain B21-058     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442169..453233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY63_RS01995 (LOY63_01995) - 443061..444329 (+) 1269 WP_010453637.1 malic enzyme-like NAD(P)-binding protein -
  LOY63_RS02000 (LOY63_02000) - 444531..446963 (-) 2433 WP_406822886.1 penicillin-binding protein 1A -
  LOY63_RS02005 (LOY63_02005) pilM 447169..448233 (+) 1065 WP_010453641.1 pilus assembly protein PilM Machinery gene
  LOY63_RS02010 (LOY63_02010) - 448233..448799 (+) 567 WP_010453642.1 PilN domain-containing protein -
  LOY63_RS02015 (LOY63_02015) pilO 448796..449419 (+) 624 WP_010453643.1 type 4a pilus biogenesis protein PilO -
  LOY63_RS02020 (LOY63_02020) pilP 449416..449943 (+) 528 WP_010453644.1 type 4a pilus biogenesis lipoprotein PilP -
  LOY63_RS02025 (LOY63_02025) pilQ 449955..452030 (+) 2076 WP_010453646.1 type IV pilus secretin PilQ Machinery gene
  LOY63_RS02030 (LOY63_02030) aroK 452035..452553 (+) 519 WP_010453648.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38141.51 Da        Isoelectric Point: 4.5877

>NTDB_id=628023 LOY63_RS02005 WP_010453641.1 447169..448233(+) (pilM) [Pseudomonas asplenii strain B21-058]
MLGLFGKKAHSLLGIDISSTSVKLLELSRSGNRYRVESYAVEPLPPNAVIEKNIAELEGVGQALTRVLLKARTSAREAAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALGL
AGLDARVVDVEAYALERSFGLLAAQLATDHDKLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSN
EEAGLAKKQGGLPDDYVIEVLQPFKDALVQQVSRSLQFFFASGQYNDVDYILLAGGTASIAGLDHLIQQRLGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=628023 LOY63_RS02005 WP_010453641.1 447169..448233(+) (pilM) [Pseudomonas asplenii strain B21-058]
GTGCTTGGACTCTTCGGCAAAAAAGCGCATTCGCTCCTGGGCATAGACATCAGCTCCACCTCCGTGAAACTCCTCGAATT
GAGCCGCTCAGGCAATCGATATCGGGTCGAGTCCTACGCGGTGGAGCCGCTGCCGCCCAATGCCGTGATCGAGAAAAACA
TCGCCGAACTCGAGGGGGTTGGCCAGGCCCTGACGCGCGTGCTGCTAAAGGCCCGGACCAGTGCGCGCGAAGCCGCGGTG
GCGGTGGCAGGTTCAGCGGTCATTACCAAGACCATCGAGATGGATGCGGGGCTCTCCGACGATGATATGGAGAACCAGCT
CAAGATAGAGGCCGACCAGTACATTCCCTATCCGCTGGAGGAGGTCGCCATCGACTTCGAGGTCCAGGGGTATTCGGCGC
GCAATCCCGAGCGGGTCGAAGTGCTCTTGGCCGCCTGTCGGAAAGAAAACGTCGAAGTGCGCGAAGCAGCCCTTGGCCTG
GCCGGACTCGATGCCCGCGTAGTTGATGTCGAAGCGTATGCGCTGGAGCGCTCCTTTGGCCTGCTGGCTGCCCAGTTGGC
TACCGATCACGACAAGTTGACGGTCGCTGTGGTCGATATCGGCGCCACCATGACCACCCTGAGTGTCCTGCATAACGGAC
GGATCATCTATACCCGCGAACAGTTGTTCGGTGGACGTCAGCTCACCGAGGAAATCCAGCGGCGCTATGGCCTCTCCAAT
GAAGAGGCCGGATTGGCGAAGAAGCAGGGCGGTTTGCCCGACGACTATGTCATTGAGGTCCTGCAGCCATTCAAGGATGC
CCTGGTGCAGCAGGTGTCGCGCTCCCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGATGTCGACTACATCCTGCTGG
CCGGCGGTACCGCTTCGATTGCCGGCCTCGATCACCTGATCCAGCAGCGCCTGGGTACTCCGACCATGGTCGCCAACCCG
TTCGCCGACATGGCATTGAGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGATGCGCCAGCCCTGATGATCGCCTGTGG
TCTGGCGTTGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H6LC79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.345

100

0.573
  comM Acinetobacter nosocomialis M2

57.345

100

0.573
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

47.324

100

0.475