Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY36_RS24250 Genome accession   NZ_CP087193
Coordinates   5412795..5414495 (+) Length   566 a.a.
NCBI ID   WP_258671184.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-019     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5407795..5419495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY36_RS24230 (LOY36_24190) - 5407897..5410851 (+) 2955 WP_258671183.1 DUF748 domain-containing protein -
  LOY36_RS24235 (LOY36_24195) - 5410851..5411147 (+) 297 WP_007938996.1 DUF2845 domain-containing protein -
  LOY36_RS24240 (LOY36_24200) - 5411475..5411831 (-) 357 WP_019650991.1 BON domain-containing protein -
  LOY36_RS24245 (LOY36_24205) - 5412158..5412568 (-) 411 WP_019650990.1 pilin -
  LOY36_RS24250 (LOY36_24210) pilB 5412795..5414495 (+) 1701 WP_258671184.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY36_RS24255 (LOY36_24215) pilC 5414498..5415715 (+) 1218 WP_019650988.1 type II secretion system F family protein Machinery gene
  LOY36_RS24260 (LOY36_24220) pilD 5415717..5416589 (+) 873 WP_258671185.1 A24 family peptidase Machinery gene
  LOY36_RS24265 (LOY36_24225) coaE 5416586..5417209 (+) 624 WP_026346004.1 dephospho-CoA kinase -
  LOY36_RS24270 (LOY36_24230) yacG 5417206..5417406 (+) 201 WP_007938961.1 DNA gyrase inhibitor YacG -
  LOY36_RS24275 (LOY36_24235) - 5417416..5417631 (-) 216 WP_007938959.1 hypothetical protein -
  LOY36_RS24280 (LOY36_24240) - 5417695..5418384 (-) 690 WP_258671186.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62930.98 Da        Isoelectric Point: 5.5491

>NTDB_id=627793 LOY36_RS24250 WP_258671184.1 5412795..5414495(+) (pilB) [Pseudomonas sp. B21-019]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRDRVSLVSYLVQNKLLKSWQVAEVASEHFGMALLDLNCLEKDTQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPSNQQVINDIQFSTGLNTEAILVEEDKLSDAIEKFFDSHATGLEEMADVDLDG
LDIESVDDQKQDTIAGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILHEVAKPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGSFTIYEPVGCNQCNAGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=627793 LOY36_RS24250 WP_258671184.1 5412795..5414495(+) (pilB) [Pseudomonas sp. B21-019]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTACTGGCCGAGCTGATCACTGATCAAAGTGCGCAACAGGC
ATATCAGCAAGCCCAACGCGATCGAGTCTCACTGGTCAGTTATCTGGTGCAAAACAAACTGCTGAAAAGCTGGCAGGTCG
CCGAGGTGGCTTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAACTGCCTGGAAAAAGACACCCAGCCCAAGGGGCTG
GTCAGCGAAAAACTGATCCGCCAGCACCACGCCCTACCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGGGTTTCCGA
TCCGAGCAATCAACAGGTCATCAACGACATCCAGTTCAGTACCGGGCTGAACACCGAAGCCATCCTGGTCGAAGAAGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGACAGCCACGCCACCGGCCTGGAAGAGATGGCCGATGTCGACCTCGACGGT
CTGGACATCGAGTCGGTCGATGACCAGAAACAGGACACCATCGCCGGACAGGATACCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAGATGCTGCTCGATGCGATCAAGAGCGGGTCTTCCGACCTGCATTTCGAACCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGATGGCATCCTGCATGAAGTCGCCAAGCCACCCACGCAACTGGCCAACCGCATTGCTTCCCGGCTC
AAAGTCATGGCCAGTCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAAATGGGCATTGATGCCCTCGGCTATGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAACAACCACAGGGG
ATGATCCTGGTGACCGGGCCCACCGGTTCAGGCAAGACCGTGTCGCTCTATACCGGGCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACATGGAGGGCATCAACCAGGTCAACGTCAATCCCAGGCAGG
GAATGGATTTCGCCCAAGCGTTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGCGACCTC
GAAACTGCCGAAATTGCCATCAAAGCCGCCCAGACCGGGCACATGGTGCTTTCAACCCTGCACACCAACAGCGCCGCGCA
AACCCTGACCCGCCTGCACAACATGGGGATTCAGGGCTTCAACATTGCGACCTCGGTCAGCCTGATCATTGCCCAGCGCC
TGGCGCGCAAGTTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGACGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAACCGGTCGGCTGCAATCAATGTAACGCCGGCTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTAAAGAACACCCCTGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAGATCGACCTCCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACCTCGGGCTTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.672

100

0.578
  pilB Acinetobacter baumannii D1279779

57.801

99.647

0.576
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.18

98.233

0.493
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.668

99.47

0.484
  pilF Thermus thermophilus HB27

36.396

100

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

93.286

0.36