Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY25_RS01870 Genome accession   NZ_CP087188
Coordinates   424071..426140 (+) Length   689 a.a.
NCBI ID   WP_258683174.1    Uniprot ID   -
Organism   Pseudomonas laurylsulfatiphila strain B21-025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419071..431140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY25_RS01850 (LOY25_01840) comM 421283..422347 (+) 1065 WP_218475837.1 pilus assembly protein PilM Machinery gene
  LOY25_RS01855 (LOY25_01845) - 422347..422913 (+) 567 WP_258683171.1 PilN domain-containing protein -
  LOY25_RS01860 (LOY25_01850) - 422910..423533 (+) 624 WP_258683172.1 type 4a pilus biogenesis protein PilO -
  LOY25_RS01865 (LOY25_01855) - 423530..424057 (+) 528 WP_258683173.1 pilus assembly protein PilP -
  LOY25_RS01870 (LOY25_01860) pilQ 424071..426140 (+) 2070 WP_258683174.1 type IV pilus secretin PilQ Machinery gene
  LOY25_RS01875 (LOY25_01865) aroK 426145..426663 (+) 519 WP_015093141.1 shikimate kinase AroK -
  LOY25_RS01880 (LOY25_01870) aroB 426814..427914 (+) 1101 WP_258683175.1 3-dehydroquinate synthase -
  LOY25_RS01885 (LOY25_01875) - 427925..429514 (+) 1590 WP_258683176.1 AAA family ATPase -

Sequence

Protein


Download         Length: 689 a.a.        Molecular weight: 74335.51 Da        Isoelectric Point: 5.2739

>NTDB_id=627654 LOY25_RS01870 WP_258683174.1 424071..426140(+) (pilQ) [Pseudomonas laurylsulfatiphila strain B21-025]
MNRIFSAFGLSLWIAMLSPMVQAANLKALDVAALPGDRVELRLSFDAPPPLPQGYMTESPARIALDLPGVASQLASKTRD
LGSGNARSATVVEAGDRTRLIINLAQSAPYDSRIEGNNLLVVVGQGVKPSTPRPAAPAMPVPARAATTPGKAIRGVDFQR
GTQGEGNVVIDLSDPSIAPDIQQREGKIIVGFAKTRLPEPLRVRLDVKDFATPVQFVNASASGDRAIISIEPGGAFDYST
YQTDSKLTVSVRPATVDDLQKRNAGAQAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQA
LDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKPDERG
SITVDERTNNIIAYQTQDRLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNRGNWNTSGVSNGLNGS
STIGTPGSTSTNSPFVDMGVANNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEA
SSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQS
KVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2070 bp        

>NTDB_id=627654 LOY25_RS01870 WP_258683174.1 424071..426140(+) (pilQ) [Pseudomonas laurylsulfatiphila strain B21-025]
ATGAACAGGATTTTCTCCGCCTTCGGCCTTTCGCTATGGATAGCGATGTTGTCGCCGATGGTACAAGCGGCCAATCTCAA
GGCGCTGGATGTCGCTGCGCTGCCGGGAGACCGGGTCGAGTTGAGGTTGTCGTTCGATGCACCGCCCCCGTTGCCTCAGG
GTTACATGACGGAGTCGCCGGCCCGGATTGCGCTGGATTTGCCGGGTGTCGCCAGTCAACTGGCCAGCAAGACTCGCGAT
CTCGGCAGTGGAAATGCCCGCAGTGCAACGGTGGTGGAGGCCGGGGACCGTACGCGATTGATCATCAACCTGGCTCAGTC
GGCCCCGTACGACTCGCGAATCGAAGGCAACAATTTGCTGGTGGTAGTCGGGCAGGGGGTCAAGCCGTCGACACCCAGGC
CGGCGGCTCCGGCAATGCCGGTTCCGGCGCGAGCCGCTACGACGCCGGGCAAGGCCATTCGGGGTGTGGATTTTCAGCGA
GGCACACAGGGTGAGGGCAACGTGGTGATCGACTTGTCGGACCCATCGATTGCGCCCGACATACAACAGCGCGAGGGCAA
GATTATTGTCGGGTTTGCCAAAACCCGACTGCCTGAGCCGCTGCGCGTGCGTCTGGATGTAAAGGATTTCGCCACGCCCG
TGCAGTTCGTCAATGCCAGCGCAAGCGGCGATCGAGCCATCATCAGCATCGAGCCCGGTGGTGCGTTCGATTACTCGACC
TATCAGACCGACAGCAAGCTCACGGTCAGCGTTCGCCCGGCGACGGTCGACGACCTGCAAAAGCGCAATGCCGGGGCACA
GGCTTACAGCGGCGAGAAGCTTTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGCAATTGATCGCCGATTTCA
CCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGTGGCATCACGTTGCGCCTGCAGAACGTGCCGTGGGATCAGGCG
CTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTCCTGCTTGTTGCGCCGGCCGATGAAAT
TGCTGCCCGTGAGCGTCAGGAACTGGAATCACAAAAGCAGATTGCCGATCTGGCGCCGTTGCGCCGGGAACTGTTGCAGG
TGAATTACGCCAAGGCGGCGGACATCGCCAAGCTCTTCCAGTCGGTGACCAGTGCTGAAGCGAAACCCGACGAACGGGGG
TCGATCACCGTCGACGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTTGATGAGCTGCGCAGGATCGT
CACGCAGTTGGATATCCCTGTGCGTCAGGTCATGATCGAGGCGCGGATTGTCGAAGCCAACGTCGACTATGACAAAAGCC
TCGGGGTGCGCTGGGGCGGATCGATACAGAACAGGGGCAACTGGAACACCTCCGGTGTCAGCAATGGACTCAACGGGTCA
TCGACCATCGGCACGCCCGGCAGTACCAGTACCAACTCACCGTTCGTCGACATGGGCGTCGCCAACAACACCTCGGGAAT
CGGCATCGCGTTCATCACCGATAACGTCTTGCTGGACCTGGAACTGACGGCCATGGAGAAAACCGGCAACGGCGAAATCG
TCTCGCAACCCAAGGTGGTCACGTCCGACAAGGAAACCGCCAAAATCCTCAAGGGCACCGAGATTCCCTATCAGGAGGCC
AGTTCCAGTGGCGCGACTTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCTTGGAGGTGACGCCGCAGATCACGCCCGACAA
CCGGATCATCATGGAGGTCAAGGTGACCAAGGACGAACCGGACTACCTGAACAAGGTCCAGGATGTACCGCCGATCAAGA
AAAACGAAGTCAACGCCAAGGTCCTGGTCAATGATGGCGAGACCATCGTCATTGGCGGCGTTTTCTCAAATACTCAGAGC
AAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGAGAA
AAAATCCGAGCTGTTGGTATTCCTCACTCCGCGTATCATGAATAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(297-343)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(524-680)

Bacterial type II/III secretion system short domain (Secretin_N)

(371-436)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.81

100

0.736
  pilQ Acinetobacter baumannii strain A118

38.585

100

0.419
  pilQ Acinetobacter baumannii D1279779

38.585

100

0.419
  comQ Acinetobacter baylyi ADP1

41.679

100

0.418
  pilQ Legionella pneumophila strain Lp02

38.905

98.113

0.382
  pilQ Legionella pneumophila strain ERS1305867

38.757

98.113

0.38