Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   LOY25_RS01850 Genome accession   NZ_CP087188
Coordinates   421283..422347 (+) Length   354 a.a.
NCBI ID   WP_218475837.1    Uniprot ID   -
Organism   Pseudomonas laurylsulfatiphila strain B21-025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416283..427347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY25_RS01840 (LOY25_01830) - 417089..418357 (+) 1269 WP_258683169.1 malic enzyme-like NAD(P)-binding protein -
  LOY25_RS01845 (LOY25_01835) - 418625..421066 (-) 2442 WP_420832650.1 penicillin-binding protein 1A -
  LOY25_RS01850 (LOY25_01840) comM 421283..422347 (+) 1065 WP_218475837.1 pilus assembly protein PilM Machinery gene
  LOY25_RS01855 (LOY25_01845) - 422347..422913 (+) 567 WP_258683171.1 PilN domain-containing protein -
  LOY25_RS01860 (LOY25_01850) - 422910..423533 (+) 624 WP_258683172.1 type 4a pilus biogenesis protein PilO -
  LOY25_RS01865 (LOY25_01855) - 423530..424057 (+) 528 WP_258683173.1 pilus assembly protein PilP -
  LOY25_RS01870 (LOY25_01860) pilQ 424071..426140 (+) 2070 WP_258683174.1 type IV pilus secretin PilQ Machinery gene
  LOY25_RS01875 (LOY25_01865) aroK 426145..426663 (+) 519 WP_015093141.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37613.09 Da        Isoelectric Point: 4.7181

>NTDB_id=627653 LOY25_RS01850 WP_218475837.1 421283..422347(+) (comM) [Pseudomonas laurylsulfatiphila strain B21-025]
MPGLFNKKSSTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPVGAVIEKNIADLEGVGLALSRALTRARTGLKGVAV
AVAGSAVITKIIEMDAALSEDELESQLKIEADQYIPYPLDEVAIDFEVQGSSARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLASRLAVSEKPLTVAVVDIGATMTTLSVLHDGRIIYTREQLFGGRQLTGEIQRRYGLTL
EQASLAQRKGGLPDDYAGEVLQPFQQALVQQVSRSLQFFFAAGQYSAVDHILLAGGTASVPGLDGLVEQQLGTPTQIANP
FSAMTLGSKVNGAALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627653 LOY25_RS01850 WP_218475837.1 421283..422347(+) (comM) [Pseudomonas laurylsulfatiphila strain B21-025]
GTGCCAGGACTCTTCAACAAAAAATCCAGTACGCTCCTGGGAATCGATATCAGCTCTACCTCGGTAAAGCTTCTGGAATT
GAGCCGCCAGGGCGAACGCTACCGGGTCGAGGCTTATGCGGTCGAGCCGTTGCCGGTCGGCGCGGTGATCGAAAAGAACA
TTGCTGACCTCGAAGGCGTGGGGCTGGCCCTTTCGCGCGCGCTGACCAGGGCCAGAACCGGCCTCAAGGGCGTGGCCGTG
GCTGTGGCAGGCTCGGCAGTGATCACCAAGATCATCGAAATGGATGCCGCTCTTTCCGAAGACGAGTTGGAAAGCCAGCT
GAAGATCGAGGCGGATCAATACATCCCTTATCCCCTGGATGAAGTCGCGATCGACTTTGAAGTCCAGGGTTCCTCCGCGC
GCAATCCGGAGCGCGTCAACGTGCTGCTCGCGGCTTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCCCTGGCCCTG
GCTGGGCTGACCGCCCGCGTAGTCGATGTCGAGGCCTATGCACTGGAGCGCTCATTCGGGTTGCTGGCCAGTCGGCTAGC
GGTCTCCGAGAAACCGTTGACGGTCGCAGTAGTGGATATCGGCGCCACCATGACCACTCTGAGCGTGCTGCACGACGGGC
GAATTATCTACACCCGCGAGCAGCTGTTCGGCGGTCGGCAGTTGACCGGGGAAATCCAGCGTCGCTATGGCCTGACGCTG
GAGCAGGCGAGTCTGGCGCAAAGGAAGGGCGGCCTGCCGGATGACTATGCCGGTGAGGTCTTGCAGCCATTTCAACAGGC
ACTGGTGCAGCAGGTTTCGCGCTCGTTGCAATTCTTCTTTGCCGCCGGCCAGTACAGCGCCGTCGACCATATCCTGCTGG
CCGGCGGTACCGCGTCGGTCCCGGGGCTGGACGGTTTGGTCGAGCAGCAATTGGGCACGCCGACCCAGATCGCGAATCCT
TTTTCCGCAATGACGCTGGGCAGCAAGGTCAACGGTGCTGCCCTGGCGAGTGATGCCCCGGCGTTGATGATCGCCTGTGG
TCTGGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

54.802

100

0.548
  pilM Acinetobacter baumannii D1279779

54.802

100

0.548
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469