Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY48_RS01930 Genome accession   NZ_CP087186
Coordinates   444947..447025 (+) Length   692 a.a.
NCBI ID   WP_258707484.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain B21-063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 439947..452025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY48_RS01910 (LOY48_01905) pilM 442162..443226 (+) 1065 WP_258707482.1 pilus assembly protein PilM Machinery gene
  LOY48_RS01915 (LOY48_01910) - 443226..443792 (+) 567 WP_056787334.1 PilN domain-containing protein -
  LOY48_RS01920 (LOY48_01915) pilO 443789..444412 (+) 624 WP_258707483.1 type 4a pilus biogenesis protein PilO -
  LOY48_RS01925 (LOY48_01920) - 444409..444933 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  LOY48_RS01930 (LOY48_01925) pilQ 444947..447025 (+) 2079 WP_258707484.1 type IV pilus secretin PilQ Machinery gene
  LOY48_RS01935 (LOY48_01930) aroK 447030..447548 (+) 519 WP_024011270.1 shikimate kinase AroK -
  LOY48_RS01940 (LOY48_01935) aroB 447701..448801 (+) 1101 WP_056787345.1 3-dehydroquinate synthase -
  LOY48_RS01945 (LOY48_01940) - 448812..450398 (+) 1587 WP_041477574.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74895.18 Da        Isoelectric Point: 5.4106

>NTDB_id=627594 LOY48_RS01930 WP_258707484.1 444947..447025(+) (pilQ) [Pseudomonas siliginis strain B21-063]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQMANKNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAPRAATAAAAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=627594 LOY48_RS01930 WP_258707484.1 444947..447025(+) (pilQ) [Pseudomonas siliginis strain B21-063]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTCGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCGCAGCCCAAGG
GCTACACAACCGAGTCACCGGCACGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGATGGCCAACAAGAATCTTGAT
CTGGGCAGCGGCAACGCGCGTACCGCAACGGTGGCCGAAGCCAAGGATCGAACCCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCGTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGACTGCGCCGCGCC
CGGCTGCCGTTGCACCGCGTGCCGCCACGGCCGCTGCGGCACCTGCCAGACCAGCCGCGCAACGAAGCAGGGCGATTCGC
GGTGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAACGTGGTCATCGATCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAACTGCCGGAAAAACTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACCCCGGTGCAATTCGTCAACGCAGCGCTCACCGGCGATCGTACGGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTACTCCACTTTTCAGACCGACAACAAGCTCACCGTGAGCGTTCGGCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAATCTCAATCTGGTGGCGAGCGATACGGTGCAGGGCGGTATCACCTTGCGCCTGCAAAAC
GTGCCGTGGGATCAGGCACTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGGCGCCCCTGC
GTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTACGACAAGAGCCTGGGCGTGCGCTGGGGTGGTTCGGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGTTCGTCGAGCACTATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAATAC
CTCGGGGATCGGCATCGCGTTCATCACCGACAACGTGCTGCTGGATCTGGAACTGACGGCGATGGAAAAGACCGGCAACG
GTGAAATCGTCTCGCAGCCGAAGGTAGTCACCTCGGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTCACACCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.207

100

0.747
  comQ Acinetobacter baylyi ADP1

41.499

100

0.416
  pilQ Acinetobacter baumannii strain A118

37.517

100

0.402
  pilQ Acinetobacter baumannii D1279779

37.517

100

0.402
  pilQ Legionella pneumophila strain Lp02

37.977

98.555

0.374
  pilQ Legionella pneumophila strain ERS1305867

37.962

97.832

0.371