Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY48_RS01910 Genome accession   NZ_CP087186
Coordinates   442162..443226 (+) Length   354 a.a.
NCBI ID   WP_258707482.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain B21-063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 437162..448226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY48_RS01900 (LOY48_01895) - 437910..439178 (+) 1269 WP_016772053.1 malic enzyme-like NAD(P)-binding protein -
  LOY48_RS01905 (LOY48_01900) - 439498..441942 (-) 2445 WP_374046344.1 penicillin-binding protein 1A -
  LOY48_RS01910 (LOY48_01905) pilM 442162..443226 (+) 1065 WP_258707482.1 pilus assembly protein PilM Machinery gene
  LOY48_RS01915 (LOY48_01910) - 443226..443792 (+) 567 WP_056787334.1 PilN domain-containing protein -
  LOY48_RS01920 (LOY48_01915) pilO 443789..444412 (+) 624 WP_258707483.1 type 4a pilus biogenesis protein PilO -
  LOY48_RS01925 (LOY48_01920) - 444409..444933 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  LOY48_RS01930 (LOY48_01925) pilQ 444947..447025 (+) 2079 WP_258707484.1 type IV pilus secretin PilQ Machinery gene
  LOY48_RS01935 (LOY48_01930) aroK 447030..447548 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38069.59 Da        Isoelectric Point: 4.7453

>NTDB_id=627593 LOY48_RS01910 WP_258707482.1 442162..443226(+) (pilM) [Pseudomonas siliginis strain B21-063]
MLGLFNKKTNPLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIADLEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPDRVNVLLAACRKENVEVREAALAL
AGLTPRVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627593 LOY48_RS01910 WP_258707482.1 442162..443226(+) (pilM) [Pseudomonas siliginis strain B21-063]
GTGCTGGGACTCTTCAATAAAAAGACCAATCCGTTGCTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTATGCGGTGGAGCCGTTGCCGGCGAACGCCGTGGTCGAAAAGAACA
TCGCCGACCTCGAAGGCGTCGGCCAGGCGCTGAGCCGGTTGCTGGTGAAAGCGCGAACCGGGGTCAAGAGCGTGGCGGTG
GCAGTGGCCGGTTCTGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGATTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGATCGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAAAACGTCGAGGTACGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCCCGCGGGTGGTGGACGTCGAGGCCTACGCCCTCGAGCGCTCGTTCGGTCTGCTGGCGACGCATCTGGC
GGCCTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGACATCGGTGCGACCATGACCACTCTCAGCGTCCTGCACAACGGCA
AGATCATCTATACCCGAGAGCAATTGTTCGGCGGACGTCAGCTCACAGAAGAGATCCAGCGCCGTTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCTTGCAGCCATTTCGCGAGGC
ACTGGTGCAGCAAGTCTCGCGTTCGCTGCAGTTCTTCTTCGCCTCCGGGCAGTACAACGCGGTCGACCATATCCTGTTGG
CCGGCGGCACCGCCTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGGCTGGACACGCCGACGCAGGTCGCCAACCCA
TTCGCCGACATGGCCTTGGGCAGTAAGGTCAACGCAGGAGCGCTGGCCAGCGACGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

48.87

100

0.489