Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY35_RS02095 Genome accession   NZ_CP087184
Coordinates   458275..460347 (+) Length   690 a.a.
NCBI ID   WP_258630146.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-028     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453275..465347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY35_RS02070 - 455280..455405 (-) 126 WP_258630133.1 hypothetical protein -
  LOY35_RS02075 (LOY35_02070) pilM 455489..456553 (+) 1065 WP_258630136.1 pilus assembly protein PilM Machinery gene
  LOY35_RS02080 (LOY35_02075) - 456553..457119 (+) 567 WP_258630137.1 PilN domain-containing protein -
  LOY35_RS02085 (LOY35_02080) pilO 457116..457739 (+) 624 WP_258630140.1 type 4a pilus biogenesis protein PilO -
  LOY35_RS02090 (LOY35_02085) - 457736..458278 (+) 543 WP_258630143.1 pilus assembly protein PilP -
  LOY35_RS02095 (LOY35_02090) pilQ 458275..460347 (+) 2073 WP_258630146.1 type IV pilus secretin PilQ Machinery gene
  LOY35_RS02100 (LOY35_02095) aroK 460352..460870 (+) 519 WP_042731985.1 shikimate kinase AroK -
  LOY35_RS02105 (LOY35_02100) aroB 461019..462119 (+) 1101 WP_258630149.1 3-dehydroquinate synthase -
  LOY35_RS02110 (LOY35_02105) - 462130..463689 (+) 1560 WP_258630150.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74827.02 Da        Isoelectric Point: 5.3057

>NTDB_id=627542 LOY35_RS02095 WP_258630146.1 458275..460347(+) (pilQ) [Pseudomonas sp. B21-028]
MNRIFTAFGVSLWMALMSPMVLGASLKTLDVAALPGDRVELKLSFDGPPPTPRGYTTEQPARIALDLPGVTNQLARKTQD
LGSGNARSATVVEAGDRTRLIIGLTQLVPYNARVEGNNLFVVVGKGAEPKSTAQAPRPAAAGVVPARSNAPVGRAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATVSIEPGGTFD
YSTYQTDNKLTVSVRPMTVEDLRKRDTDRAAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSGVTNGS
STTIGTPGSTSTNQPFVDMGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDLVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=627542 LOY35_RS02095 WP_258630146.1 458275..460347(+) (pilQ) [Pseudomonas sp. B21-028]
ATGAACAGGATTTTTACCGCCTTCGGTGTTTCGCTATGGATGGCGCTGATGTCACCGATGGTGCTTGGGGCGAGCCTGAA
GACGCTGGATGTCGCGGCTTTGCCGGGGGACCGGGTCGAGTTGAAGCTTTCTTTCGATGGGCCACCGCCGACGCCCCGTG
GCTATACCACCGAACAACCGGCCCGGATTGCCCTGGACCTGCCAGGGGTGACCAATCAGCTGGCGCGCAAGACCCAGGAC
CTGGGCAGCGGCAATGCACGCAGCGCCACCGTGGTCGAGGCGGGGGACCGTACCCGGCTGATCATCGGCCTCACCCAGTT
GGTGCCGTATAACGCTCGGGTCGAAGGTAATAATCTGTTCGTGGTGGTCGGTAAGGGCGCGGAGCCGAAATCTACCGCCC
AGGCCCCACGTCCGGCGGCTGCCGGTGTGGTGCCAGCCAGGAGCAACGCGCCGGTGGGCCGGGCCATTCGTGGCGTGGAC
TTCCAGCGCGGGACCCAGGGTGAAGGCAACGTGGTGATCGACCTGTCCGACCCGTCCATTGCGCCGGATATCCAGGAGCG
GGAAGGCAAGATCATTCTCAACTTCGCCAGGACCCAATTGCCCGAACCGTTGCGGGTGCGCCTGGACGTCAAGGACTTCG
CCACCCCGGTGCAGTTCGTCAACGCCAGCGCCGGTTCCGACCGGGCCACCGTCAGCATCGAGCCCGGCGGTACCTTCGAT
TATTCGACCTACCAGACCGACAACAAGCTGACCGTCAGCGTCCGGCCCATGACGGTCGAGGACTTGCGCAAGCGCGATAC
CGACCGTGCGGCCTACAACGGTGAAAAGCTGTCCCTGAATTTTCAGGACATCGAAGTGCGCTCGGTCCTGCAGCTGATTG
CCGATTTCACCAACCTGAACCTGGTGGCAAGCGACACGGTGCAGGGCGGTATCACCTTGCGGTTGCAGAATGTTCCCTGG
GACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTGCTGTTGGTGGCCCCCGC
CGATGAGATTGCGGCTCGCGAGCGCCAGGAACTGGAATCCCAGAAGCAGATTGCCGACCTGGCGCCGCTGCGGCGAGAGC
TGTTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAAGCGAAAGCCGAT
GAGCGCGGTACGATCACGGTGGATGAGCGAACCAATAACATCATTGCCTACCAGACCCAGGACCGGCTCGACGAATTGCG
CCGGATCGTGGCGCAGCTGGATATTCCGGTGCGCCAGGTAATGATCGAGGCCCGGATCGTTGAGGCCAACGTCGATTACG
ATAAAAGCCTGGGCGTGCGTTGGGGCGGCTCGGTGCAGAACAAGGGCAACTGGAACACTTCGGGCGTGACCAACGGCAGC
TCCACCACCATCGGCACGCCGGGCAGCACCAGCACCAACCAGCCGTTCGTCGACATGGGCGCGTCGGGCAATACCTCGGG
TATCGGCATTGCGTTCATCACCGACAACGTACTGCTGGATCTGGAACTGACAGCCATGGAAAAAACCGGTAACGGGGAAA
TCGTTTCCCAGCCCAAGGTAGTGACCTCCGACAAGGAGACCGCAAAAATCCTCAAGGGCACGGAAATTCCCTACCAGGAA
GCCAGTTCCAGTGGGGCCACCTCGGTGTCGTTCAAGGAAGCCTCGCTATCGCTGGAGGTCACCCCGCAAATCACACCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTCCAGGAAGTACCACCGATCA
AGAAGAACGAAGTCAACGCCAAGGTGTTGGTCAATGATGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACTCAG
AGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATCTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.587

100

0.752
  comQ Acinetobacter baylyi ADP1

40.138

100

0.422
  pilQ Acinetobacter baumannii strain A118

38.566

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.566

100

0.413
  pilQ Legionella pneumophila strain Lp02

39.286

97.391

0.383
  pilQ Legionella pneumophila strain ERS1305867

39.046

97.246

0.38