Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY35_RS02075 Genome accession   NZ_CP087184
Coordinates   455489..456553 (+) Length   354 a.a.
NCBI ID   WP_258630136.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-028     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450489..461553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY35_RS02060 (LOY35_02060) - 451419..452687 (+) 1269 WP_258630128.1 malic enzyme-like NAD(P)-binding protein -
  LOY35_RS02065 (LOY35_02065) - 452836..455280 (-) 2445 WP_408981237.1 penicillin-binding protein 1A -
  LOY35_RS02070 - 455280..455405 (-) 126 WP_258630133.1 hypothetical protein -
  LOY35_RS02075 (LOY35_02070) pilM 455489..456553 (+) 1065 WP_258630136.1 pilus assembly protein PilM Machinery gene
  LOY35_RS02080 (LOY35_02075) - 456553..457119 (+) 567 WP_258630137.1 PilN domain-containing protein -
  LOY35_RS02085 (LOY35_02080) pilO 457116..457739 (+) 624 WP_258630140.1 type 4a pilus biogenesis protein PilO -
  LOY35_RS02090 (LOY35_02085) - 457736..458278 (+) 543 WP_258630143.1 pilus assembly protein PilP -
  LOY35_RS02095 (LOY35_02090) pilQ 458275..460347 (+) 2073 WP_258630146.1 type IV pilus secretin PilQ Machinery gene
  LOY35_RS02100 (LOY35_02095) aroK 460352..460870 (+) 519 WP_042731985.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38211.77 Da        Isoelectric Point: 4.9112

>NTDB_id=627541 LOY35_RS02075 WP_258630136.1 455489..456553(+) (pilM) [Pseudomonas sp. B21-028]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVIEKNIAELEGVGQALSRVLVKARTPSRSVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVLGVSPRNNERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FSNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627541 LOY35_RS02075 WP_258630136.1 455489..456553(+) (pilM) [Pseudomonas sp. B21-028]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTCTGGGCATCGACATCAGCTCCACATCGGTGAAGCTGCTCGAGCT
GAGCCGCCAGGGTGACCGTTACCGTGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAACGCCGTGATCGAGAAGAACA
TCGCCGAGCTCGAGGGGGTCGGGCAGGCGTTGTCCCGGGTTCTGGTCAAGGCCAGGACGCCCTCGCGCAGCGTCGCGGTG
GCCGTGGCGGGGTCGGCGGTGATCACCAAGACCATCGAGATGGATGCCGGGCTTTCCGACGACGAGATGGAGAACCAGCT
CAAGATCGAGGCTGACCAGTACATTCCCTATCCACTGGATGAAGTCGCCATCGATTTCGAGGTCCTGGGTGTGTCGCCGC
GCAACAACGAGCGGGTCGAGGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAAGTTCGCGAGGCGGCGCTGGCGCTG
GCGGGGCTGACGGCCCGGGTGGTCGACGTGGAAGCCTACGCATTGGAGCGCGCCTTCGGCCTGCTCGCCACGCAACTGGC
CGCGTCCCAGGAGCGGTTGACCGTTGCGGTGATCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGCTCACCGAGGAAATCCAGCGGCGCTACGGCCTGACCCTC
GAACAGGCCGGCCTGGCAAAGAAGCAGGGGGGCTTGCCGGACGATTATCTCAGCGAGGTGCTGCAGCCTTTTCGCGAGGC
GTTGGTGCAGCAGGTTTCGCGGTCCCTGCAGTTTTTCTTCGCTTCGGGCCAGTACAGCGCGGTGGACCACATCCTGTTGG
CTGGAGGTACGGCGTCAGTCGCCGGGCTGGACCGGTTGATCGAACAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTTCCAACATGGCCCTGGGCAGCAAGGTCAATGCCGGCGCCCTGGCCAGTGATGCGCCAGCGCTGATGATCGCTTGCGG
ACTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483