Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY57_RS01970 Genome accession   NZ_CP087177
Coordinates   447687..449765 (+) Length   692 a.a.
NCBI ID   WP_258640125.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain B21-039     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442687..454765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY57_RS01950 (LOY57_01945) pilM 444902..445966 (+) 1065 WP_258640121.1 pilus assembly protein PilM Machinery gene
  LOY57_RS01955 (LOY57_01950) - 445966..446532 (+) 567 WP_042608694.1 PilN domain-containing protein -
  LOY57_RS01960 (LOY57_01955) pilO 446529..447152 (+) 624 WP_042608695.1 type 4a pilus biogenesis protein PilO -
  LOY57_RS01965 (LOY57_01960) - 447149..447673 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  LOY57_RS01970 (LOY57_01965) pilQ 447687..449765 (+) 2079 WP_258640125.1 type IV pilus secretin PilQ Machinery gene
  LOY57_RS01975 (LOY57_01970) aroK 449770..450288 (+) 519 WP_024011270.1 shikimate kinase AroK -
  LOY57_RS01980 (LOY57_01975) aroB 450441..451541 (+) 1101 WP_065615444.1 3-dehydroquinate synthase -
  LOY57_RS01985 (LOY57_01980) - 451552..453138 (+) 1587 WP_064363264.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74949.25 Da        Isoelectric Point: 5.4106

>NTDB_id=627375 LOY57_RS01970 WP_258640125.1 447687..449765(+) (pilQ) [Pseudomonas moraviensis strain B21-039]
MNRIFSTLGFSLWIALVSPMVMAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLANRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAPRAAPAAAAPAKFSVPRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSVDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=627375 LOY57_RS01970 WP_258640125.1 447687..449765(+) (pilQ) [Pseudomonas moraviensis strain B21-039]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAACCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCCCAGCCCAAGG
GCTACACGACCGAGTCGCCCGCGCGTATTGCACTGGATCTGCCGGGGGTGGCCAGCCAGTTGGCCAACAGAAATCTTGAT
CTGGGCAGCGGCAATGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCATACAGCACGCGGGTCGAAGGCAATAATCTGTTCGTGGTGGTTGGTCAGGGCGCCTCCGCGACCGCGCCGCGCC
CGGCCGCCGTTGCACCACGTGCCGCCCCGGCCGCTGCGGCGCCCGCGAAATTTTCTGTGCCGCGCAGCCGGGCAATTCGC
GGCGTCGATTTCCAGCGCGGTACCGAGGGCGAAGGCAACGTGGTCATCGATCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAACACGACGGCAAGATCATCCTCAGCTTCGCCCGCACCCAATTGCCGGAAAAGCTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTATTCGACCTTCCAGACTGATAACAAGCTCACGGTGAGCGTTCGCCCGATGAGTGTCGACGATCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTGTCGCTGAATTTTCAGGACATCGATGTACGCTCGGTGCTGC
AACTGATCGCAGACTTCACCAATCTCAACCTCGTGGCAAGCGACACGGTGCAGGGCGGCATCACGTTGCGCCTGCAGAAC
GTGCCGTGGGACCAGGCACTGGATCTGGTGTTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGCAACGTGCTACTGGT
GGCGCCGGCCGATGAAATTGCCGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCATTGC
GTCGCGAGCTGCTGCAAGTCAATTACGCCAAGGCTGCGGACATTGCCAAACTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAATTACGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGACAAGAGCCTCGGCGTACGCTGGGGCGGCTCGGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGCTCATCGAGCACCATCGGTACGCCGGGCAGCACCAGCACCAACTCGCCCTTCGTCGACATGGGCACGGTCAACAATAC
CTCCGGGATCGGTATCGCGTTCATCACCGACAACGTGTTGCTGGACCTGGAACTTACGGCGATGGAAAAGACCGGCAACG
GCGAGATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATACCGTAT
CAGGAGGCCAGCTCCAGTGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTCACTCCGCAGATCAC
GCCTGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTGAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.207

100

0.747
  comQ Acinetobacter baylyi ADP1

41.04

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.038

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.038

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.85

98.121

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.814

97.832

0.37