Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY57_RS01950 Genome accession   NZ_CP087177
Coordinates   444902..445966 (+) Length   354 a.a.
NCBI ID   WP_258640121.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain B21-039     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 439902..450966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY57_RS01940 (LOY57_01935) - 440612..441880 (+) 1269 WP_042608691.1 malic enzyme-like NAD(P)-binding protein -
  LOY57_RS01945 (LOY57_01940) - 442238..444682 (-) 2445 WP_408980791.1 penicillin-binding protein 1A -
  LOY57_RS01950 (LOY57_01945) pilM 444902..445966 (+) 1065 WP_258640121.1 pilus assembly protein PilM Machinery gene
  LOY57_RS01955 (LOY57_01950) - 445966..446532 (+) 567 WP_042608694.1 PilN domain-containing protein -
  LOY57_RS01960 (LOY57_01955) pilO 446529..447152 (+) 624 WP_042608695.1 type 4a pilus biogenesis protein PilO -
  LOY57_RS01965 (LOY57_01960) - 447149..447673 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  LOY57_RS01970 (LOY57_01965) pilQ 447687..449765 (+) 2079 WP_258640125.1 type IV pilus secretin PilQ Machinery gene
  LOY57_RS01975 (LOY57_01970) aroK 449770..450288 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38048.59 Da        Isoelectric Point: 4.8182

>NTDB_id=627374 LOY57_RS01950 WP_258640121.1 444902..445966(+) (pilM) [Pseudomonas moraviensis strain B21-039]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGCKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVTEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627374 LOY57_RS01950 WP_258640121.1 444902..445966(+) (pilM) [Pseudomonas moraviensis strain B21-039]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGTCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTAGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGTGTCGGCCAGGCGCTCAGCCGGTTGCTGGTCAAGGCACGCACCGGGTGCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCGGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGATGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGTGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGCGAGGCGGCGCTGGCGCTC
GCCGGCCTGACTGCACGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGGCTGCTGGCGACGCATCTGGC
TGCTTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTAAGCGTCCTGCATAACGGCA
AGATCATCTATACCCGCGAGCAACTCTTTGGCGGGCGCCAGCTCACCGAAGAGATCCAGCGCCGCTATGGCCTCACTGTC
GAGCAGGCGGGCCTGGCGAAAAAGCAGGGCGGCCTGCCTGACGATTACGTGACCGAAGTCCTGCAGCCATTTCGCGATGC
ACTGGTGCAGCAGGTTTCGCGTTCGCTGCAGTTCTTCTTCGCCTCTGGCCAGTACAACGCCGTCGACCATATCCTGCTGG
CCGGCGGCACGGCCTCTGTGCCCGGGCTGGATCGCCTGATCGAACAGCGCTTGAACACGCCGACCCAGGTTGCCAACCCG
TTTGCCGACATGGCCCTGGGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGTGATGCGCCCGCATTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483