Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY56_RS01890 Genome accession   NZ_CP087168
Coordinates   404223..406298 (+) Length   691 a.a.
NCBI ID   WP_258619260.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 399223..411298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY56_RS01870 (LOY56_01870) comM 401438..402502 (+) 1065 WP_258619254.1 pilus assembly protein PilM Machinery gene
  LOY56_RS01875 (LOY56_01875) - 402502..403065 (+) 564 WP_258619255.1 PilN domain-containing protein -
  LOY56_RS01880 (LOY56_01880) pilO 403062..403685 (+) 624 WP_258619258.1 type 4a pilus biogenesis protein PilO -
  LOY56_RS01885 (LOY56_01885) - 403682..404209 (+) 528 WP_258619259.1 pilus assembly protein PilP -
  LOY56_RS01890 (LOY56_01890) pilQ 404223..406298 (+) 2076 WP_258619260.1 type IV pilus secretin PilQ Machinery gene
  LOY56_RS01895 (LOY56_01895) aroK 406303..406821 (+) 519 WP_258619262.1 shikimate kinase AroK -
  LOY56_RS01900 (LOY56_01900) aroB 406972..408072 (+) 1101 WP_258619264.1 3-dehydroquinate synthase -
  LOY56_RS01905 (LOY56_01905) - 408083..409684 (+) 1602 WP_258619268.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74882.00 Da        Isoelectric Point: 5.7805

>NTDB_id=627143 LOY56_RS01890 WP_258619260.1 404223..406298(+) (pilQ) [Pseudomonas sp. B21-048]
MNRIFSTFGISLWIAFLSPMVQAASLKALDVAALPGDRVELKLSFDGPPPQPHGYTTEQPARIALDLPGVTSQLANKNRD
LGGGNARSATVVEAKDRTRLIINLTQLAPYNSRVEGNNLFVVVGQGAGSKVPKPITSTSRAATAAPAPARANAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIIVGFTKTQLPERLRVRLDVKDFATPVQFVNASATGDRATISIEPSG
AFDYSTYQTDNKLTVSVRPMTVDDLQKRNAERFAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQKGNWNTSGVNG
SSTTIGTPGSTSTNSPFVDLGTSANTSGIGIAFITDNVLLDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=627143 LOY56_RS01890 WP_258619260.1 404223..406298(+) (pilQ) [Pseudomonas sp. B21-048]
ATGAACAGGATTTTTTCAACCTTCGGTATTTCGCTATGGATAGCGTTTCTGTCGCCGATGGTTCAGGCGGCCAGCCTCAA
AGCGCTGGATGTCGCTGCGCTGCCCGGTGACCGTGTCGAGTTGAAGTTGTCGTTCGACGGGCCGCCGCCGCAACCCCATG
GTTACACGACGGAGCAGCCTGCGCGGATTGCGCTGGACCTGCCGGGGGTCACCAGTCAGCTGGCGAACAAGAATCGCGAT
CTGGGCGGCGGCAATGCCCGCAGTGCCACGGTAGTAGAGGCTAAAGACCGCACACGACTGATCATCAACCTGACTCAATT
GGCCCCGTACAACTCACGCGTTGAAGGCAACAATCTGTTCGTGGTCGTCGGGCAGGGGGCGGGTAGCAAGGTGCCCAAGC
CGATTACCAGCACCTCGCGTGCAGCCACTGCAGCGCCAGCGCCCGCCAGGGCCAATGCGCCCGTGGGCAAAGCCATCCGG
GGCGTGGACTTCCAGCGCGGCACCCAGGGTGAGGGCAATGTGGTCATCGATCTGTCGGACCCGTCCATCGCCCCGGACAT
CCAGGAGCGCGACGGCAAGATCATCGTCGGTTTCACCAAGACCCAACTGCCTGAACGGTTGCGTGTACGCCTCGACGTCA
AGGATTTCGCCACGCCAGTGCAGTTCGTCAACGCCAGTGCCACGGGCGATCGGGCCACAATCAGTATCGAGCCCAGCGGC
GCCTTCGATTATTCGACCTATCAGACCGACAACAAATTGACCGTCAGCGTCCGGCCGATGACCGTCGATGACTTGCAAAA
ACGTAACGCCGAACGCTTTGCCTACAGCGGTGAAAAACTCTCGCTGAATTTTCAGGACATTGATGTGCGCTCGGTTCTGC
AACTGATTGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATAACTTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGTTGGACCTGGTGCTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGTAACGTGCTGCTGGT
GGCACCAGCCGATGAGATCGCCGCTCGGGAGCGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGCTAC
GTCGCGAGCTGTTACAGGTCAACTACGCCAAAGCCGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCTGAGGCG
AAAATCGACGAGCGAGGGTCAATCACTGTCGATGAGCGAACCAACAACATCATTGCTTACCAGACCCAGGATCGGCTCGA
CGAACTGCGTCGCATCGTTGCGCAGCTGGATATTCCGGTGCGTCAGGTAATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGATAAAAGCCTTGGCGTACGCTGGGGTGGTTCGGTGCAGAAGGGCAACTGGAACACGTCCGGGGTCAACGGC
TCTTCGACCACCATTGGTACACCGGGCAGCACCAGTACCAATTCGCCGTTCGTTGACTTGGGGACCTCTGCCAATACCTC
GGGGATCGGCATCGCCTTCATTACCGACAATGTGCTGCTGGACCTTGAGCTTTCGGCTATGGAGAAAACCGGCAACGGGG
AAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAAATACTAAAGGGCACCGAGATTCCCTATCAG
GAAGCCAGCTCCAGCGGCGCCACCTCGGTGTCGTTCAAGGAGGCTTCTCTGTCCCTGGAAGTGACCCCGCAGATCACGCC
AGACAACCGAATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAGAAAAACGAGGTCAACGCCAAAGTGTTGGTCAACGATGGCGAGACCATCGTCATTGGCGGAGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-121)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.249

100

0.757
  comQ Acinetobacter baylyi ADP1

41.541

100

0.437
  pilQ Acinetobacter baumannii D1279779

38.8

100

0.421
  pilQ Acinetobacter baumannii strain A118

38.8

100

0.421
  pilQ Legionella pneumophila strain Lp02

38.067

98.842

0.376
  pilQ Legionella pneumophila strain ERS1305867

37.941

98.408

0.373