Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY49_RS01920 Genome accession   NZ_CP087167
Coordinates   438184..440262 (+) Length   692 a.a.
NCBI ID   WP_258604560.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain B21-050     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433184..445262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY49_RS01900 (LOY49_01895) pilM 435399..436463 (+) 1065 WP_024011266.1 pilus assembly protein PilM Machinery gene
  LOY49_RS01905 (LOY49_01900) - 436463..437029 (+) 567 WP_016772051.1 PilN domain-containing protein -
  LOY49_RS01910 (LOY49_01905) pilO 437026..437649 (+) 624 WP_071174143.1 type 4a pilus biogenesis protein PilO -
  LOY49_RS01915 (LOY49_01910) - 437646..438170 (+) 525 WP_160768498.1 pilus assembly protein PilP -
  LOY49_RS01920 (LOY49_01915) pilQ 438184..440262 (+) 2079 WP_258604560.1 type IV pilus secretin PilQ Machinery gene
  LOY49_RS01925 (LOY49_01920) aroK 440267..440785 (+) 519 WP_024011270.1 shikimate kinase AroK -
  LOY49_RS01930 (LOY49_01925) aroB 440938..442038 (+) 1101 WP_041477573.1 3-dehydroquinate synthase -
  LOY49_RS01935 (LOY49_01930) - 442049..443635 (+) 1587 WP_122611067.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74929.27 Da        Isoelectric Point: 5.4106

>NTDB_id=627113 LOY49_RS01920 WP_258604560.1 438184..440262(+) (pilQ) [Pseudomonas atacamensis strain B21-050]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQLANRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASAAAPRPAAVAPRAATAAVAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GLSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=627113 LOY49_RS01920 WP_258604560.1 438184..440262(+) (pilQ) [Pseudomonas atacamensis strain B21-050]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCGCCGGCGCGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGTTGGCCAACAGGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCTTACAGCACTCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGGCTGCGCCGCGCC
CGGCTGCCGTTGCACCGCGTGCCGCCACGGCCGCTGTGGCACCTGCCAGACCAGCCGCGCAACGAAGCAGGGCGATTCGC
GGCGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAACGTAGTCATCGACCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAGCACGATGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTGCCGGAAAAGCTGCGCGTGCGTCTGGACGTCA
AGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCAGCGTCGAGCCCAGCGGC
ACCTTCGACTACTCCACGTTTCAGACCGACAACAAGCTCACCGTCAGCGTGCGGCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAATCTCAATCTGGTGGCGAGCGATACGGTGCAGGGCGGTATCACTTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGTTGAAAACCAAGGGCTTGGACAAGCGCAAGATCGGCAATGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCTCGTGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGGCGCCCCTGC
GTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCCGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCCTCGGGGGTCAAC
GGTTTGTCGAGCACTATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAATAC
CTCGGGGATCGGCATCGCTTTCATCACCGACAACGTGCTGCTGGATCTCGAACTGACGGCGATGGAAAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAAGAGGCCTCGCTGTCGCTGGAAGTCACTCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACTTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.927

100

0.744
  comQ Acinetobacter baylyi ADP1

41.787

100

0.419
  pilQ Acinetobacter baumannii D1279779

37.787

100

0.405
  pilQ Acinetobacter baumannii strain A118

37.787

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.27

98.555

0.377
  pilQ Legionella pneumophila strain Lp02

38.144

98.121

0.374