Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY49_RS01900 Genome accession   NZ_CP087167
Coordinates   435399..436463 (+) Length   354 a.a.
NCBI ID   WP_024011266.1    Uniprot ID   V8RDF4
Organism   Pseudomonas atacamensis strain B21-050     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430399..441463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY49_RS01890 (LOY49_01885) - 431042..432466 (+) 1425 WP_258604556.1 IS1182 family transposase -
  LOY49_RS01895 (LOY49_01890) - 432735..435179 (-) 2445 WP_373877946.1 penicillin-binding protein 1A -
  LOY49_RS01900 (LOY49_01895) pilM 435399..436463 (+) 1065 WP_024011266.1 pilus assembly protein PilM Machinery gene
  LOY49_RS01905 (LOY49_01900) - 436463..437029 (+) 567 WP_016772051.1 PilN domain-containing protein -
  LOY49_RS01910 (LOY49_01905) pilO 437026..437649 (+) 624 WP_071174143.1 type 4a pilus biogenesis protein PilO -
  LOY49_RS01915 (LOY49_01910) - 437646..438170 (+) 525 WP_160768498.1 pilus assembly protein PilP -
  LOY49_RS01920 (LOY49_01915) pilQ 438184..440262 (+) 2079 WP_258604560.1 type IV pilus secretin PilQ Machinery gene
  LOY49_RS01925 (LOY49_01920) aroK 440267..440785 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38045.57 Da        Isoelectric Point: 4.7539

>NTDB_id=627112 LOY49_RS01900 WP_024011266.1 435399..436463(+) (pilM) [Pseudomonas atacamensis strain B21-050]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627112 LOY49_RS01900 WP_024011266.1 435399..436463(+) (pilM) [Pseudomonas atacamensis strain B21-050]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTGGAGCCGTTGCCGGCGAACGCTGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTTGGCCAGGCACTGAGCCGCCTGCTGGTCAAGGCGCGTACCGGGGTCAAGAGCGTGGCGGTG
GCAGTGGCGGGTTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTGGCAACGCATCTGGC
GGCCTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGATATTGGCGCGACCATGACCACTCTCAGCGTCCTGCACAACGGCA
AGATCATCTATACCCGCGAGCAATTGTTCGGCGGACGCCAGCTCACCGAAGAGATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCAGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCCTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAAGTCTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCATATCCTGCTGG
CCGGCGGCACCGCCTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGTCTGGACACGCCGACGCAGGTCGCCAACCCA
TTCGCCGACATGGCATTGGGCAGCAAGGTCAACGCAGGAGCGCTGGCCAGCGACGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8RDF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483