Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KT882_RS06745 Genome accession   NZ_CP086370
Coordinates   1537644..1538834 (+) Length   396 a.a.
NCBI ID   WP_267478642.1    Uniprot ID   -
Organism   Pseudoduganella aquatica strain HC52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1532644..1543834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KT882_RS06720 - 1533524..1534237 (+) 714 WP_228895044.1 YggS family pyridoxal phosphate-dependent enzyme -
  KT882_RS06725 proC 1534305..1535174 (+) 870 WP_228895046.1 pyrroline-5-carboxylate reductase -
  KT882_RS06730 - 1535273..1535620 (-) 348 WP_161071629.1 YqjD family protein -
  KT882_RS06735 ubiA 1535662..1536516 (-) 855 WP_161071628.1 4-hydroxybenzoate octaprenyltransferase -
  KT882_RS06740 - 1536571..1537515 (-) 945 WP_161071627.1 hydrogen peroxide-inducible genes activator -
  KT882_RS06745 pilU 1537644..1538834 (+) 1191 WP_267478642.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KT882_RS06750 - 1538847..1539329 (+) 483 WP_161071625.1 adenylyltransferase/cytidyltransferase family protein -
  KT882_RS06755 - 1539429..1540295 (-) 867 WP_321170331.1 transposase -
  KT882_RS06760 trmL 1540356..1540826 (-) 471 WP_161071623.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  KT882_RS06765 - 1540862..1541641 (-) 780 WP_161071622.1 ComF family protein -
  KT882_RS06770 - 1541700..1542632 (+) 933 WP_228895048.1 methyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43946.28 Da        Isoelectric Point: 6.3206

>NTDB_id=624557 KT882_RS06745 WP_267478642.1 1537644..1538834(+) (pilU) [Pseudoduganella aquatica strain HC52]
MSSTFGHEEAQAYIHKLLKVMHQTGGSDLFISADFPPSIKSQGAMKPLSQQRLTGEVTRALAMAIMNDKQRAEFAAEMEC
NFAISLPDVCRFRVNVLMQQQNVAMVIRTIASEIPNFEKLDLPEVLKEVIMTKRGLVLVVGGTGSGKSTTLAAMIDYRNS
NSAGHIITVEDPVEYVHKNKGCLVTHREVGVDTHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLKAIVSQRLVRTEDGKGRKAAIEILLNTPTIAEMIFKGNFHAIKEIMAKS
RELGMCTFDQALYELYNKGYIAYEEALRNADSANGLRLQIKLRGDRKEPGEGGASRPVGELSMQIDAEEEDAEPGH

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=624557 KT882_RS06745 WP_267478642.1 1537644..1538834(+) (pilU) [Pseudoduganella aquatica strain HC52]
ATGTCATCCACATTTGGCCATGAAGAAGCGCAGGCCTACATCCACAAGCTGCTCAAGGTGATGCACCAGACTGGCGGCTC
GGACCTGTTCATCTCCGCCGACTTCCCGCCCAGCATCAAGTCGCAGGGCGCGATGAAACCGCTCAGCCAGCAGCGCCTGA
CCGGCGAAGTCACGCGCGCGCTGGCCATGGCCATCATGAACGACAAGCAGCGCGCCGAATTCGCCGCCGAGATGGAATGC
AATTTTGCCATCTCGCTGCCGGACGTGTGCCGCTTCCGCGTCAACGTGCTGATGCAGCAGCAGAACGTGGCCATGGTGAT
ACGCACCATCGCCTCCGAGATTCCGAATTTTGAAAAGCTGGACCTGCCCGAGGTGCTGAAGGAGGTCATCATGACCAAGC
GCGGGCTGGTGCTGGTGGTGGGCGGCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATAGACTACCGCAACAGC
AATTCGGCCGGCCACATCATCACGGTGGAAGACCCGGTGGAGTACGTGCACAAGAACAAGGGCTGCCTGGTGACGCACCG
CGAAGTGGGCGTGGACACGCATTCCTGGCACAACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTTTGATCG
GCGAGATCCGCGACACCGAGACCATGGAACACGCCATCGCCTTCGCCGAGACCGGCCATTTGTGCCTGGGCACGCTGCAC
GCCAACAACGCCAACCAGACCATGGACCGCATCATCAACTTCTTCCCGGAGGAGCGGCGCAACCAGCTGCTGATGGACTT
GTCGTCGAATCTGAAGGCCATCGTGTCGCAGCGGCTGGTGCGCACCGAGGACGGCAAGGGGCGCAAGGCGGCCATTGAAA
TACTGCTCAACACGCCGACGATTGCCGAGATGATCTTCAAGGGCAACTTCCACGCCATCAAGGAAATCATGGCCAAGTCG
CGCGAGCTGGGCATGTGCACCTTCGACCAGGCCCTGTACGAGCTCTATAATAAGGGCTACATTGCCTACGAAGAAGCGCT
GCGCAACGCCGACTCGGCCAACGGCCTGCGGCTGCAGATCAAGCTGCGCGGCGACCGCAAGGAGCCGGGCGAGGGCGGCG
CCAGCCGGCCGGTTGGCGAGCTGAGCATGCAAATCGACGCGGAAGAAGAAGACGCCGAGCCCGGCCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.105

95.96

0.548
  pilU Acinetobacter baylyi ADP1

52.011

94.192

0.49
  pilU Vibrio cholerae strain A1552

53.959

86.111

0.465
  pilT Legionella pneumophila strain Lp02

44.97

85.354

0.384
  pilT Legionella pneumophila strain ERS1305867

44.97

85.354

0.384
  pilT Pseudomonas aeruginosa PAK

43.62

85.101

0.371
  pilT Pseudomonas stutzeri DSM 10701

42.433

85.101

0.361