Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KM317_RS14480 Genome accession   NZ_CP086333
Coordinates   3515325..3517061 (-) Length   578 a.a.
NCBI ID   WP_053833735.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. arrhenatheri strain LMG 727     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3510325..3522061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM317_RS14460 (KM317_14460) - 3511464..3513077 (+) 1614 WP_053833738.1 sensor histidine kinase -
  KM317_RS14465 (KM317_14465) pilR 3513152..3514540 (+) 1389 WP_053833737.1 sigma-54-dependent transcriptional regulator Regulator
  KM317_RS14470 (KM317_14470) - 3514705..3515001 (+) 297 WP_053833736.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KM317_RS14475 (KM317_14475) - 3515004..3515297 (+) 294 WP_009580422.1 addiction module antidote protein -
  KM317_RS14480 (KM317_14480) pilB 3515325..3517061 (-) 1737 WP_053833735.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KM317_RS14485 (KM317_14485) - 3517249..3517692 (+) 444 WP_186008602.1 hypothetical protein -
  KM317_RS14490 (KM317_14490) pilA 3517848..3518273 (-) 426 WP_053833734.1 pilin Machinery gene
  KM317_RS14495 (KM317_14495) pilC 3518654..3519913 (+) 1260 WP_053833733.1 type II secretion system F family protein Machinery gene
  KM317_RS14500 (KM317_14500) - 3519920..3520783 (+) 864 WP_053833732.1 prepilin peptidase -
  KM317_RS14505 (KM317_14505) coaE 3520797..3521417 (+) 621 WP_053833731.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62948.27 Da        Isoelectric Point: 6.2661

>NTDB_id=624359 KM317_RS14480 WP_053833735.1 3515325..3517061(-) (pilB) [Xanthomonas translucens pv. arrhenatheri strain LMG 727]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELLHKHQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGRLEVGAGDEDMGSGVDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRVLDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNEEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=624359 KM317_RS14480 WP_053833735.1 3515325..3517061(-) (pilB) [Xanthomonas translucens pv. arrhenatheri strain LMG 727]
ATGAACGCTGTGACATCCGCCAATCTCGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGTTGCACAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGACGCGCTGGGC
GACGACGACGAGGCCATGGGCAGGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGCGGCGTCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCGGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAAATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTGTCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATTCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGCGAGGCGGAAGTCGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGAAGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTTCGCCAGTCGGCGCTGCTC
AAGGCACGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.754

98.27

0.538
  pilB Acinetobacter baumannii D1279779

54.049

98.27

0.531
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilB Vibrio cholerae strain A1552

48.873

99.827

0.488
  pilF Neisseria gonorrhoeae MS11

48.768

98.27

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.82

97.924

0.458
  pilB Vibrio campbellii strain DS40M4

46.221

98.443

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

90.311

0.362