Detailed information NTG0622390

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL045_RS09225
• Genome accession: NZ_CP086083
• Coordinates: 1906488..1907135 (-)
• Length: 215 a.a.
• NCBI ID: WP_098393299.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain EIP20A
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1901488..1912135

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL045_RS09200 (LL045_09185)	atpF	1901558..1902064 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LL045_RS09205 (LL045_09190)	atpB	1902079..1902792 (-)	714	WP_038601808.1	F0F1 ATP synthase subunit A	-
LL045_RS09210 (LL045_09195)	-	1902837..1903052 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LL045_RS09215 (LL045_09200)	-	1903236..1904012 (-)	777	WP_029344289.1	alpha/beta hydrolase family protein	-
LL045_RS09220 (LL045_09205)	comEC	1904297..1906507 (-)	2211	WP_394530103.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LL045_RS09225 (LL045_09210)	comEA	1906488..1907135 (-)	648	WP_098393299.1	ComEA family DNA-binding protein	Machinery gene
LL045_RS09230 (LL045_09215)	-	1907195..1908556 (-)	1362	WP_311865731.1	ABC transporter permease	-
LL045_RS09235 (LL045_09220)	-	1908553..1909485 (-)	933	WP_394530105.1	ABC transporter ATP-binding protein	-
LL045_RS09240 (LL045_09225)	-	1909589..1909987 (-)	399	WP_038601821.1	hypothetical protein	-
LL045_RS09245 (LL045_09230)	-	1910091..1910654 (-)	564	WP_098393297.1	GNAT family N-acetyltransferase	-
LL045_RS09250 (LL045_09235)	-	1910827..1912005 (-)	1179	WP_038601825.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23606.83 Da
• Isoelectric Point: 4.9882

>NTDB_id=622390 LL045_RS09225 WP_098393299.1 1906488..1907135(-) (comEA) [Lactococcus lactis subsp. lactis strain EIP20A]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVIKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLIDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTAKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=622390 LL045_RS09225 WP_098393299.1 1906488..1907135(-) (comEA) [Lactococcus lactis subsp. lactis strain EIP20A]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATACTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTTATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCATTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAACCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAATTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGCAAAAATAAATATCAATCAAGCAGAT
TTAACAGAATTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGAACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTATGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (152-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.229	100	0.763
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	37.004	100	0.391