Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LK401_RS01615 Genome accession   NZ_CP085952
Coordinates   322988..323437 (-) Length   149 a.a.
NCBI ID   WP_011271973.1    Uniprot ID   Q4QNP4
Organism   Haemophilus influenzae strain FDAARGOS_1560     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 317988..328437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK401_RS01595 (LK401_01595) rho 318376..319638 (+) 1263 WP_005629550.1 transcription termination factor Rho -
  LK401_RS01600 (LK401_01600) pilD 319692..320384 (-) 693 WP_038440789.1 A24 family peptidase Machinery gene
  LK401_RS01605 (LK401_01605) pilC 320381..321601 (-) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  LK401_RS01610 (LK401_01610) pilB 321594..322991 (-) 1398 WP_038440787.1 GspE/PulE family protein Machinery gene
  LK401_RS01615 (LK401_01615) pilA 322988..323437 (-) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LK401_RS01620 (LK401_01620) ampD 323552..324112 (+) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LK401_RS01625 (LK401_01625) corC 324734..325633 (+) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  LK401_RS01630 (LK401_01630) lnt 325656..327182 (+) 1527 WP_080291958.1 apolipoprotein N-acyltransferase -
  LK401_RS01635 (LK401_01635) rsmE 327235..327972 (+) 738 WP_038440782.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15572.89 Da        Isoelectric Point: 8.0922

>NTDB_id=621573 LK401_RS01615 WP_011271973.1 322988..323437(-) (pilA) [Haemophilus influenzae strain FDAARGOS_1560]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=621573 LK401_RS01615 WP_011271973.1 322988..323437(-) (pilA) [Haemophilus influenzae strain FDAARGOS_1560]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

100

100

1
  pilA Haemophilus influenzae Rd KW20

91.946

100

0.919
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

41.497

98.658

0.409
  pilA2 Legionella pneumophila strain ERS1305867

41.497

98.658

0.409
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376