Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LK420_RS08595 Genome accession   NZ_CP085949
Coordinates   1770098..1771492 (-) Length   464 a.a.
NCBI ID   WP_021035704.1    Uniprot ID   -
Organism   Haemophilus influenzae strain FDAARGOS_1562     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1765098..1776492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK420_RS08560 (LK420_08560) - 1765348..1765554 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  LK420_RS08565 (LK420_08565) - 1765628..1765834 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  LK420_RS08570 (LK420_08570) cueR 1765911..1766297 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  LK420_RS08575 (LK420_08575) metJ 1766311..1766628 (-) 318 WP_021035707.1 met regulon transcriptional regulator MetJ -
  LK420_RS08580 (LK420_08580) rho 1766876..1768138 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  LK420_RS08585 (LK420_08585) pilD 1768192..1768884 (-) 693 WP_041175383.1 prepilin peptidase Machinery gene
  LK420_RS08590 (LK420_08590) pilC 1768881..1770101 (-) 1221 WP_021035705.1 type II secretion system F family protein Machinery gene
  LK420_RS08595 (LK420_08595) pilB 1770098..1771492 (-) 1395 WP_021035704.1 GspE/PulE family protein Machinery gene
  LK420_RS08600 (LK420_08600) pilA 1771489..1771938 (-) 450 WP_021035703.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LK420_RS08605 (LK420_08605) ampD 1772053..1772607 (+) 555 WP_021035702.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LK420_RS08610 (LK420_08610) corC 1773239..1774138 (+) 900 WP_021035700.1 CNNM family magnesium/cobalt transport protein CorC -
  LK420_RS08615 (LK420_08615) lnt 1774161..1775690 (+) 1530 WP_021035699.1 apolipoprotein N-acyltransferase -
  LK420_RS08620 (LK420_08620) rsmE 1775740..1776477 (+) 738 WP_041175381.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52922.35 Da        Isoelectric Point: 5.6301

>NTDB_id=621529 LK420_RS08595 WP_021035704.1 1770098..1771492(-) (pilB) [Haemophilus influenzae strain FDAARGOS_1562]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
TFLRQDPDIIMLSEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYQGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=621529 LK420_RS08595 WP_021035704.1 1770098..1771492(-) (pilB) [Haemophilus influenzae strain FDAARGOS_1562]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGGTATTTACGATCTCGCCAGATTTATG
GGAACGTAATCAGCAACAACAGTCTCTGCTCTTACGTTATTTTGCGTTACCACTCAAAGAAGAAAATAATCGTCTTTGGT
TAGGTGTCGATTCCCTTTCTAATCTTTCCGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAGACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAATTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAGCCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACACGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
ACTTTCTTACGCCAAGATCCCGATATTATTATGCTAAGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTAGTACTCTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAACGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAATCAAGGAATGACGGATAATA
AAGAACTTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

95.905

100

0.959
  pilB Haemophilus influenzae Rd KW20

95.896

99.784

0.957
  pilB Glaesserella parasuis strain SC1401

56.798

98.276

0.558
  pilB Vibrio cholerae strain A1552

40.779

100

0.429
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

38.945

100

0.414
  pilB Acinetobacter baylyi ADP1

37.702

100

0.403
  pilB Acinetobacter baumannii D1279779

38.462

100

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.752

100

0.371
  pilF Neisseria gonorrhoeae MS11

36.134

100

0.371
  pilF Thermus thermophilus HB27

35.404

100

0.369