Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LIS66_RS01990 Genome accession   NZ_CP085885
Coordinates   452711..454789 (+) Length   692 a.a.
NCBI ID   WP_227973499.1    Uniprot ID   -
Organism   Pseudomonas sp. HN2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447711..459789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS66_RS01970 (LIS66_01970) pilM 449926..450990 (+) 1065 WP_042608693.1 pilus assembly protein PilM Machinery gene
  LIS66_RS01975 (LIS66_01975) - 450990..451556 (+) 567 WP_042608694.1 PilN domain-containing protein -
  LIS66_RS01980 (LIS66_01980) pilO 451553..452176 (+) 624 WP_227973498.1 type 4a pilus biogenesis protein PilO -
  LIS66_RS01985 (LIS66_01985) - 452173..452697 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  LIS66_RS01990 (LIS66_01990) pilQ 452711..454789 (+) 2079 WP_227973499.1 type IV pilus secretin PilQ Machinery gene
  LIS66_RS01995 (LIS66_01995) aroK 454794..455312 (+) 519 WP_024011270.1 shikimate kinase AroK -
  LIS66_RS02000 (LIS66_02000) aroB 455465..456565 (+) 1101 WP_227973500.1 3-dehydroquinate synthase -
  LIS66_RS02005 (LIS66_02005) - 456576..458162 (+) 1587 WP_039756683.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74935.23 Da        Isoelectric Point: 5.4106

>NTDB_id=621120 LIS66_RS01990 WP_227973499.1 452711..454789(+) (pilQ) [Pseudomonas sp. HN2]
MNRIFSTLGFSLWIALVSPMVMAANLKALDVAALPGDRVELKLSFDGPPPQLKGYTTESPARIALDLPGVASQLANRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPATVAPRAAPAAAAPAKSSVPRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSVDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=621120 LIS66_RS01990 WP_227973499.1 452711..454789(+) (pilQ) [Pseudomonas sp. HN2]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAACCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCCCAGCTCAAGG
GCTACACGACCGAGTCGCCCGCGCGTATTGCACTGGATCTGCCGGGGGTGGCCAGCCAGTTGGCCAACAGAAATCTTGAT
CTGGGCAGCGGCAATGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCATACAGCACGCGGGTCGAAGGCAATAATCTGTTCGTGGTGGTTGGTCAGGGCGCCTCCGCGACCGCGCCGCGCC
CGGCCACCGTTGCACCACGTGCCGCCCCGGCCGCTGCGGCGCCCGCGAAATCTTCTGTGCCGCGCAGCCGGGCAATTCGC
GGCGTCGATTTCCAGCGCGGTACCGAGGGCGAAGGCAACGTGGTCATCGATCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAACACGACGGCAAGATCATCCTCAGCTTCGCCCGCACCCAATTGCCGGAAAAGCTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTATTCGACCTTCCAGACTGATAACAAGCTCACGGTGAGCGTTCGCCCGATGAGTGTCGACGATCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGTGACAAGCTGTCGCTGAATTTTCAGGACATCGACGTACGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAACCTCAACCTCGTGGCAAGCGACACGGTGCAGGGCGGCATCACGTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCACTGGATCTGGTGTTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGCAACGTGCTACTGGT
GGCGCCGGCCGATGAAATTGCCGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCATTGC
GTCGCGAGCTGCTGCAAGTCAATTACGCCAAGGCTGCGGACATTGCCAAACTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAATTACGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGACAAGAGCCTCGGCGTACGCTGGGGCGGCTCGGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGCTCATCGAGCACCATCGGTACGCCGGGCAGCACCAGCACCAACTCGCCCTTCGTCGACATGGGCACGGTCAACAATAC
CTCCGGGATCGGTATCGCGTTCATCACCGACAACGTGTTGCTGGACCTGGAACTGACGGCGATGGAAAAGACCGGCAACG
GCGAGATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATACCGTAT
CAGGAGGCCAGCTCCAGTGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTCACTCCGCAGATCAC
GCCTGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTGAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.346

100

0.749
  comQ Acinetobacter baylyi ADP1

41.329

100

0.413
  pilQ Acinetobacter baumannii D1279779

37.903

100

0.408
  pilQ Acinetobacter baumannii strain A118

37.903

100

0.408
  pilQ Legionella pneumophila strain Lp02

37.85

98.121

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.814

97.832

0.37