Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LIS66_RS01970 Genome accession   NZ_CP085885
Coordinates   449926..450990 (+) Length   354 a.a.
NCBI ID   WP_042608693.1    Uniprot ID   -
Organism   Pseudomonas sp. HN2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444926..455990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS66_RS01960 (LIS66_01960) - 445636..446904 (+) 1269 WP_042608691.1 malic enzyme-like NAD(P)-binding protein -
  LIS66_RS01965 (LIS66_01965) - 447262..449706 (-) 2445 WP_371919887.1 penicillin-binding protein 1A -
  LIS66_RS01970 (LIS66_01970) pilM 449926..450990 (+) 1065 WP_042608693.1 pilus assembly protein PilM Machinery gene
  LIS66_RS01975 (LIS66_01975) - 450990..451556 (+) 567 WP_042608694.1 PilN domain-containing protein -
  LIS66_RS01980 (LIS66_01980) pilO 451553..452176 (+) 624 WP_227973498.1 type 4a pilus biogenesis protein PilO -
  LIS66_RS01985 (LIS66_01985) - 452173..452697 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  LIS66_RS01990 (LIS66_01990) pilQ 452711..454789 (+) 2079 WP_227973499.1 type IV pilus secretin PilQ Machinery gene
  LIS66_RS01995 (LIS66_01995) aroK 454794..455312 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38062.62 Da        Isoelectric Point: 4.8225

>NTDB_id=621119 LIS66_RS01970 WP_042608693.1 449926..450990(+) (pilM) [Pseudomonas sp. HN2]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGCKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVTEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=621119 LIS66_RS01970 WP_042608693.1 449926..450990(+) (pilM) [Pseudomonas sp. HN2]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGTCGCCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTAGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGTGTCGGCCAGGCGCTCAGCCGGTTGCTGGTCAAGGCACGCACCGGATGCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCGGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGATGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGCGAGGCGGCGCTGGCGCTC
GCCGGCCTGACTGCACGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGGCTGCTGGCGACGCATCTGGC
TGCTTCCCAGGAGCGCCTGACCGTGGCGGTGGTCGACATCGGCGCGACGATGACCACCCTCAGCGTCCTGCATAACGGCA
AGATCATCTATACCCGCGAGCAACTCTTTGGCGGGCGTCAGCTCACCGAAGAGATCCAGCGCCGCTATGGCCTCACTGTC
GAGCAGGCGGGCCTGGCGAAAAAGCAGGGCGGCCTGCCTGACGATTACGTGACCGAAGTCCTGCAGCCATTTCGCGAGGC
ACTGGTGCAGCAGGTTTCGCGTTCGCTGCAGTTCTTCTTCGCCTCTGGCCAGTACAACGCCGTCGACCATATCCTGCTGG
CCGGCGGCACGGCCTCTGTACCCGGGCTGGATCGCCTGATCGAACAGCGCTTGAACACGCCGACTCAGGTTGCCAACCCG
TTTGCCGACATGGCCCTGGGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGTGATGCGCCCGCATTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48