Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HWQ47_RS25090 Genome accession   NZ_CP085636
Coordinates   5761015..5762724 (+) Length   569 a.a.
NCBI ID   WP_269968686.1    Uniprot ID   -
Organism   Shewanella sp. MTB7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5756015..5767724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ47_RS25070 (HWQ47_25095) ampE 5756920..5757771 (-) 852 WP_269968683.1 beta-lactamase regulator AmpE -
  HWQ47_RS25075 (HWQ47_25100) ampD 5757826..5758416 (-) 591 WP_269971856.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HWQ47_RS25080 (HWQ47_25105) nadC 5758767..5759657 (+) 891 WP_269968684.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HWQ47_RS25085 (HWQ47_25110) - 5760472..5760945 (+) 474 WP_269968685.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HWQ47_RS25090 (HWQ47_25115) pilB 5761015..5762724 (+) 1710 WP_269968686.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HWQ47_RS25095 (HWQ47_25120) pilC 5762816..5764081 (+) 1266 WP_269971857.1 type II secretion system F family protein Machinery gene
  HWQ47_RS25100 (HWQ47_25125) pilD 5764356..5765267 (+) 912 WP_269968687.1 A24 family peptidase Machinery gene
  HWQ47_RS25105 (HWQ47_25130) coaE 5765303..5765908 (+) 606 WP_269968688.1 dephospho-CoA kinase -
  HWQ47_RS25110 (HWQ47_25135) zapD 5765917..5766651 (+) 735 WP_269968689.1 cell division protein ZapD -
  HWQ47_RS25115 (HWQ47_25140) yacG 5766724..5766936 (+) 213 WP_269968690.1 DNA gyrase inhibitor YacG -
  HWQ47_RS25120 (HWQ47_25145) mutT 5766940..5767344 (+) 405 WP_269968691.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63150.32 Da        Isoelectric Point: 4.9940

>NTDB_id=619013 HWQ47_RS25090 WP_269968686.1 5761015..5762724(+) (pilB) [Shewanella sp. MTB7]
MPTTGLHLGLSTLFIRKNLLSEEQIASAITKSRQSTQSLVSTIISEKLISAREIAELCYEEYGTPLLDLDEFDVASIPED
FLNKKLIEKHKCLPLFKRGNRLYIGTSDPTNISALEDFQFSAGLHAEAILVEDDKLTKALDKILEDDISGLDISGIDEET
LAGIEVEDTSKRVEDTAGESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSNRISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRQLYEEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFPAALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQHEEVPEHELLRLGF
KQTQIDEDLTLFKPIGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATQAKEQGMRDLRASGLLKVIQGVTSI
TEINRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=619013 HWQ47_RS25090 WP_269968686.1 5761015..5762724(+) (pilB) [Shewanella sp. MTB7]
ATGCCAACAACAGGGCTACACCTAGGTCTATCTACACTTTTTATTCGTAAAAATCTGCTAAGTGAAGAGCAGATAGCCTC
TGCCATCACCAAATCCAGACAAAGCACACAATCGCTCGTTTCAACTATCATCTCAGAAAAACTGATCTCAGCCCGTGAAA
TCGCTGAGCTTTGCTATGAGGAATATGGCACGCCACTGCTGGACTTGGATGAATTTGATGTCGCCAGTATTCCTGAAGAT
TTTCTTAATAAAAAACTGATCGAGAAACATAAATGCTTGCCCCTGTTTAAGCGAGGTAATCGCCTCTATATCGGCACTTC
TGATCCCACCAACATTTCAGCTCTGGAAGATTTTCAATTCAGTGCAGGTCTGCATGCTGAAGCTATATTGGTCGAAGATG
ATAAACTCACCAAAGCATTAGATAAAATACTAGAAGATGATATATCGGGCTTAGACATCAGCGGCATAGATGAAGAGACT
CTAGCCGGCATTGAAGTTGAAGATACCAGTAAACGCGTTGAGGATACGGCGGGTGAAAGTAGTGACGATGCGCCCATTGT
TATCTACATTAATAAGATTCTCACCGATGCTATTCGTAAAGGTGCCTCGGATCTGCATTTTGAGCCCTATGAAAAACGCT
ATCGTATTCGCTTTCGTATCGATGGCATACTGCACGAAGTCTCCGAGCCTCCGGTTAACTTATCTAATCGTATATCCGCA
CGATTAAAGGTGATGTCAAAACTTGATATCGCCGAACGCCGAGTGCCACAAGATGGCCGTATCAAGATGAAACTGTCACG
CACTAAGTCGATTGATTTCCGTGTCAGTACGCTCCCTACTATCTGGGGAGAGAAGATTGTGATGCGTATTCTAGATTCCT
CTTCGGCGCAGCTTGGCATCGAGAAATTAGGCTATGAAGATGATCAACGGCAACTCTACGAAGAGATGCTGGCTAAACCC
CAAGGCATGATTCTAGTCACTGGACCAACGGGTTCAGGTAAAACCGTCTCGCTGTATACTGGTCTCAATATCCTCAACAC
TGAAGAGCGCAATATTTCCACTGCTGAAGATCCGGTAGAGATTAACTTAGAAGGGGTTAATCAGGTTCACATCAATCTTA
AGGCTGGGCTGACCTTCCCTGCCGCACTTAGATCTTTCCTGCGTCAAGACCCAGATGTAGTCATGGTCGGTGAAATTCGC
GATTTAGAAACCGCCGAAATCGCCATTAAAGCTGCTCAAACCGGTCACTTAGTGCTCTCCACCCTGCACACTAATTCAGC
GGCTGAAACACTCACCCGCTTGATCAACATGGGCGTGCCTGGCTACAACATCGCCAGCTCAGTCAACCTTATCATAGCCC
AGCGATTAGCACGTCGATTGTGTCCTGAGTGTAAGCAACATGAAGAGGTCCCCGAACATGAGCTATTACGTCTCGGTTTT
AAACAAACGCAGATTGACGAAGATCTAACCCTATTCAAACCCATAGGTTGCGACTTATGTTCCGGCGGTTACAAGGGGCG
AGTCGGTATCTATGAGGTGATGAAGATGTCGGATGAGATAGCAAGGACCATTATGGAGGGAGGCAACTCTCTACAGATAG
CCACTCAAGCCAAAGAGCAAGGAATGCGAGATCTGCGCGCTTCAGGCCTATTGAAGGTCATTCAAGGTGTGACCAGTATC
ACCGAGATCAATCGCGTCACTAGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.111

98.594

0.573
  pilB Acinetobacter baumannii D1279779

56.863

98.594

0.561
  pilB Legionella pneumophila strain ERS1305867

56.05

98.77

0.554
  pilB Vibrio cholerae strain A1552

53.014

99.121

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

51.761

99.824

0.517
  pilB Vibrio campbellii strain DS40M4

51.596

99.121

0.511
  pilF Neisseria gonorrhoeae MS11

47.35

99.473

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.95

97.715

0.371
  pilF Thermus thermophilus HB27

36.898

98.594

0.364