Detailed information NTG0616132

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: LG309_RS15835
• Genome accession: NZ_CP085231
• Coordinates: 3397305..3399008 (-)
• Length: 567 a.a.
• NCBI ID: WP_404395066.1
• Uniprot ID: -
• Organism: Stutzerimonas chloritidismutans strain ADH2-893
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3382794..3397302	3397305..3399008	flank	3

Gene organization within MGE regions

Location: 3382794..3399008

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG309_RS15745 (LG309_15720)	rsmI	3382794..3383657 (-)	864	WP_041014820.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
LG309_RS15750 (LG309_15725)	-	3383782..3385584 (+)	1803	WP_404395050.1	penicillin-binding protein activator	-
LG309_RS15755 (LG309_15730)	-	3385584..3385946 (+)	363	WP_014821349.1	YraN family protein	-
LG309_RS15760 (LG309_15735)	-	3386056..3386649 (+)	594	WP_013981954.1	phosphoheptose isomerase	-
LG309_RS15765 (LG309_15740)	-	3386646..3387224 (+)	579	WP_404395053.1	BON domain-containing protein	-
LG309_RS15770 (LG309_15745)	-	3387264..3387662 (-)	399	WP_404395055.1	ClpXP protease specificity-enhancing factor	-
LG309_RS15775 (LG309_15750)	-	3387675..3388292 (-)	618	WP_090519234.1	glutathione S-transferase N-terminal domain-containing protein	-
LG309_RS15780 (LG309_15755)	-	3388381..3389160 (-)	780	WP_278379752.1	cytochrome c1	-
LG309_RS15785 (LG309_15760)	-	3389160..3390371 (-)	1212	WP_041014823.1	cytochrome bc complex cytochrome b subunit	-
LG309_RS15790 (LG309_15765)	petA	3390371..3390964 (-)	594	WP_014821354.1	ubiquinol-cytochrome c reductase iron-sulfur subunit	-
LG309_RS15795 (LG309_15770)	rpsI	3391221..3391613 (-)	393	WP_003302797.1	30S ribosomal protein S9	-
LG309_RS15800 (LG309_15775)	rplM	3391628..3392056 (-)	429	WP_014821355.1	50S ribosomal protein L13	-
LG309_RS15805 (LG309_15780)	-	3392488..3393525 (+)	1038	WP_404395059.1	NADP(H)-dependent aldo-keto reductase	-
LG309_RS15810 (LG309_15785)	-	3393588..3394268 (+)	681	WP_045666681.1	energy-coupling factor ABC transporter permease	-
LG309_RS15815 (LG309_15790)	yacG	3394279..3394476 (-)	198	WP_003302802.1	DNA gyrase inhibitor YacG	-
LG309_RS15820 (LG309_15795)	coaE	3394473..3395081 (-)	609	WP_003302803.1	dephospho-CoA kinase	-
LG309_RS15825 (LG309_15800)	pilD	3395213..3396082 (-)	870	WP_404395062.1	prepilin peptidase	Machinery gene
LG309_RS15830 (LG309_15805)	pilC	3396085..3397302 (-)	1218	WP_404395064.1	type II secretion system F family protein	Machinery gene
LG309_RS15835 (LG309_15810)	pilB	3397305..3399008 (-)	1704	WP_404395066.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62402.59 Da
• Isoelectric Point: 5.7757

>NTDB_id=616132 LG309_RS15835 WP_404395066.1 3397305..3399008(-) (pilB) [Stutzerimonas chloritidismutans strain ADH2-893]
MNENAPLGGLARQLVLVGQLDEKIALQAQQQALRNQVPLVTWLVQNKLVKSRALVDIAGEQFGVPFFDLGALDKETQPKD
LVSEKLVRQHRALPLQRRGNKLYMAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKFFESASTALDGLGDADLD
GLDVEISGEVKDDEGPHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEEDQKELYLTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVDVPRDVLLKEGFP
EARIGSFKLYGPVGCDSCKNGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARKDGFNDLRTSGLVKAMHGVTSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=616132 LG309_RS15835 WP_404395066.1 3397305..3399008(-) (pilB) [Stutzerimonas chloritidismutans strain ADH2-893]
ATGAACGAAAACGCGCCCCTTGGCGGCTTGGCCCGGCAGCTGGTTCTCGTAGGCCAGCTCGACGAAAAAATTGCGCTGCA
GGCTCAGCAGCAAGCCTTGCGTAACCAGGTGCCTTTGGTGACATGGTTGGTGCAGAACAAGCTGGTCAAAAGTCGGGCGC
TTGTCGATATCGCCGGTGAGCAGTTTGGCGTTCCGTTCTTTGACTTGGGTGCGTTGGATAAAGAGACCCAGCCGAAGGAT
TTGGTCAGCGAGAAGCTGGTACGCCAGCATCGCGCACTGCCGCTACAGCGACGTGGAAATAAGCTGTATATGGCGGTTTC
CGACCCAACCAATCATCAAGCAATCAGCGACATCCAGTTCAACACTGGTCTGTCGGTCGAGGCGGTGCTGGTCGAAGACG
ATAAGCTTGGCGAGGCGATCGAGAAGTTCTTCGAGTCGGCGTCCACTGCGCTTGATGGTCTCGGAGATGCTGATCTTGAC
GGGTTGGATGTCGAAATCAGCGGAGAGGTGAAGGATGACGAAGGGCCTCACAATGAGGCCGATGATGCGCCGGTCGTGCG
CTTCGTTAACAAGATGTTGCTCGATGCGATCAAGGGTGGATCGTCAGACCTGCACTTCGAGCCCTATGAAAAATCCTATC
GGGTGCGTTTCCGTACCGATGGCATGTTGCACGAAGTCGCTCGTCCTCCGGTCCAGTTGGTTAGCCGGATTTCTGCGCGT
TTGAAGGTAATGGCCAGTCTTGATATCGCTGAGCGGCGCAAACCGCAGGACGGGCGTATCAAGATGAAGGTTTCGAAAAC
CAAATCCATCGACTTCCGCGTCAATACGCTGCCCACCCTGTGGGGCGAAAAAATCGTAATGCGGATCCTGGACTCGTCAA
GCGCCAAGATGGGTATTGATGCGCTGGGCTATGAGGAAGACCAGAAAGAGCTGTACCTAACCGCGTTGAAGCAACCGCAA
GGGATGATTCTGGTTACCGGTCCGACAGGTTCGGGCAAGACTGTTTCGCTGTATACCGGCCTGAATATTCTCAATACGGT
AGACGTAAATATTTCCACTGCGGAAGACCCCGTGGAAATCAACCTGGAGGGTATCAATCAGGTTAACGTCAACCCGAGGC
AGGGCATGGACTTTGCGCAGGCGCTGAGGGCTTTCCTTCGGCAGGATCCGGATATCATCATGGTCGGTGAGATCCGAGAC
TTGGAGACTGCTGAGATCGCCATCAAGGCGGCGCAGACCGGCCATATGGTCATGTCGACATTGCACACCAATAGCGCAGC
TGAAACGCTCACCCGGCTGCGCAATATGGGGGTGCCGGCGTTCAATATCGCTACCTCGGTGAACCTGATCATTGCGCAGC
GCTTGGCACGCAAACTATGCCCCAGCTGCAAGCAAGAAGTTGATGTGCCGCGTGATGTCCTGCTCAAGGAGGGTTTTCCA
GAGGCGCGGATCGGTTCGTTCAAGCTTTACGGGCCGGTTGGTTGCGATAGCTGCAAGAACGGTTACAAAGGCCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCAGCACTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGGAAAGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTCAAGGCCATGCATGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	59.609	99.118	0.591
pilB	Acinetobacter baylyi ADP1	57.592	100	0.582
pilB	Legionella pneumophila strain ERS1305867	54.816	100	0.552
pilB	Vibrio cholerae strain A1552	50.444	99.295	0.501
pilB	Vibrio parahaemolyticus RIMD 2210633	51.099	96.296	0.492
pilF	Neisseria gonorrhoeae MS11	51.381	95.767	0.492
pilB	Vibrio campbellii strain DS40M4	50.182	96.649	0.485
pilF	Thermus thermophilus HB27	38.204	100	0.383
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.4	94.004	0.37
pilB/pilB1	Synechocystis sp. PCC 6803	39.499	91.534	0.362