Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LG327_RS08160 Genome accession   NZ_CP085217
Coordinates   1738439..1740499 (+) Length   686 a.a.
NCBI ID   WP_404368282.1    Uniprot ID   -
Organism   Marinobacter sp. strain QJJ-212     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1733439..1745499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG327_RS08140 (LG327_08140) pilM 1735587..1736651 (+) 1065 WP_404368271.1 pilus assembly protein PilM Machinery gene
  LG327_RS08145 (LG327_08145) - 1736651..1737217 (+) 567 WP_404368273.1 PilN domain-containing protein -
  LG327_RS08150 (LG327_08150) - 1737214..1737825 (+) 612 WP_404368276.1 type 4a pilus biogenesis protein PilO -
  LG327_RS08155 (LG327_08155) - 1737828..1738373 (+) 546 WP_404368279.1 pilus assembly protein PilP -
  LG327_RS08160 (LG327_08160) pilQ 1738439..1740499 (+) 2061 WP_404368282.1 type IV pilus secretin PilQ Machinery gene
  LG327_RS08165 (LG327_08165) aroK 1740623..1741189 (+) 567 WP_404368284.1 shikimate kinase AroK -
  LG327_RS08170 (LG327_08170) aroB 1741182..1742276 (+) 1095 WP_404368286.1 3-dehydroquinate synthase -
  LG327_RS08175 (LG327_08175) - 1742294..1743841 (+) 1548 WP_404368289.1 AAA family ATPase -

Sequence

Protein


Download         Length: 686 a.a.        Molecular weight: 74022.15 Da        Isoelectric Point: 4.3327

>NTDB_id=615947 LG327_RS08160 WP_404368282.1 1738439..1740499(+) (pilQ) [Marinobacter sp. strain QJJ-212]
MFRKLTLCVSVIAFGMMSGLATAVTLEDVSFSSLPGERTEVTLKFDGQPPEPTGYTIERPARIAIDLRDTRSGLDTRNLS
LGSGNAQSMTVVETKDRTRLIFNLVELTPYTSSRSDNSIVLTIGEGAGTSAAAPTTATAARSGSGVVDGGIRDVDFRRGD
DGEGRVVIQLSDRKTPVDLSESAGRIRVVLEDTEVPPELRRRLDVTDFATPVSRVDTYMEGNDAVIEIRPEGSYDYIAYQ
ADTTFTVSVEPLTEEEAESRREDRFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTVSGSITLRLQNVPWDQALD
LILKTKGLDKRQIGNVLLVAPADEIAARERLELETSRQIAELAPVSLDIIQVNYAKAADIVSLIQEDAELISDRGFISSD
GRTNTISVRETVEKREQIRRLVNTWDVPVRQVLIEARLVRAQSNVARNLGVRWGGAAFDVSDGNVTSLGGSLNTVREGRT
AAGGGSGAISFPDALAVDLGVTQGGASSFAIGFGGDDFLIDLELSALESDGKAEIVSQPRVVTADRQAASIKSGEEIPFQ
EASSSGATSVSFKEAVLALEVTPQITPDDKIIMDLVVSQDSRGEVTQGIPAINTNEVTTQVLVRNGETVVLGGIFQSEVS
TTVTKTPFLGDIPYLGRLFKRTENVDERSELLVFITPKILKGDLIE

Nucleotide


Download         Length: 2061 bp        

>NTDB_id=615947 LG327_RS08160 WP_404368282.1 1738439..1740499(+) (pilQ) [Marinobacter sp. strain QJJ-212]
ATGTTCAGAAAACTAACTTTATGCGTCAGCGTGATTGCTTTTGGAATGATGTCCGGACTGGCGACTGCTGTGACCCTGGA
GGATGTGTCTTTTTCATCACTCCCTGGAGAGCGAACGGAGGTGACCCTCAAATTTGACGGCCAGCCACCGGAGCCGACTG
GTTACACTATTGAGCGTCCTGCACGCATTGCAATAGATCTGCGGGATACCCGGAGCGGGCTGGATACCCGAAACCTGTCC
CTGGGGTCGGGGAATGCACAGAGCATGACGGTTGTGGAGACAAAGGATCGCACACGCCTGATATTCAACCTTGTCGAGCT
TACCCCTTATACATCCAGCCGCAGCGACAATTCCATTGTTCTGACAATTGGTGAAGGTGCTGGCACTTCTGCGGCTGCGC
CGACCACTGCAACTGCTGCCAGGTCCGGATCAGGCGTCGTGGACGGTGGAATCAGGGATGTGGATTTCCGGCGCGGCGAT
GACGGTGAAGGTCGGGTCGTCATCCAGCTTTCAGACCGTAAAACACCGGTGGACCTTTCCGAGTCAGCCGGCCGTATTCG
TGTAGTGCTGGAAGACACCGAAGTTCCTCCGGAGCTTCGCCGCCGTCTGGATGTCACCGATTTTGCTACTCCCGTGAGCC
GTGTTGATACCTACATGGAAGGTAACGACGCAGTCATAGAGATTCGGCCCGAAGGCAGTTACGACTACATAGCGTATCAG
GCGGACACTACCTTTACCGTAAGTGTCGAGCCGCTGACCGAAGAAGAAGCGGAGAGCCGCCGGGAAGATCGCTTCCCCTA
TACAGGCGACAAGCTTTCCCTGAACTTCCAGGATATCGAAGTGCGCTCCGTGCTGCAGCTGATTGCTGATTTCACCGGCC
TGAACCTGGTGGCCAGCGACACGGTCAGCGGCAGCATCACGCTGCGCCTCCAGAACGTTCCCTGGGATCAGGCTCTTGAC
CTTATCCTGAAGACCAAGGGTCTGGACAAACGGCAGATAGGCAACGTCCTGCTGGTTGCACCGGCAGATGAAATTGCCGC
CCGGGAGCGGCTGGAGCTGGAAACCAGCAGGCAGATTGCAGAACTGGCACCTGTGTCACTGGACATTATCCAGGTCAATT
ACGCCAAGGCTGCTGACATAGTTTCCCTCATTCAGGAAGACGCGGAGCTGATCTCAGACCGGGGCTTCATCTCCTCGGAT
GGTCGTACCAATACCATCAGTGTCCGGGAAACCGTGGAGAAACGGGAACAGATCAGGCGTCTGGTGAATACCTGGGACGT
TCCCGTACGCCAGGTGCTGATTGAGGCCAGGCTTGTGCGGGCTCAGTCTAATGTTGCCAGAAACCTTGGTGTTCGCTGGG
GCGGTGCAGCGTTCGATGTTAGCGACGGAAACGTAACGTCGCTTGGCGGGAGCCTGAATACCGTTCGGGAAGGCCGGACA
GCCGCCGGTGGTGGCTCCGGTGCGATAAGTTTCCCTGACGCACTGGCAGTGGACCTGGGCGTCACTCAGGGCGGTGCATC
GTCCTTTGCAATCGGTTTTGGTGGTGATGATTTCCTGATTGATCTGGAACTGTCGGCCCTTGAGAGTGATGGCAAGGCCG
AAATCGTATCCCAGCCAAGAGTGGTTACGGCGGATCGACAGGCGGCGTCCATCAAGTCCGGTGAAGAAATTCCCTTCCAG
GAAGCGTCATCAAGTGGTGCAACGTCCGTATCCTTCAAAGAGGCGGTGCTGGCACTGGAAGTTACTCCCCAGATCACGCC
GGATGACAAGATCATCATGGACCTGGTGGTCAGTCAGGATTCCAGAGGTGAGGTCACCCAGGGGATTCCGGCCATCAATA
CCAATGAAGTCACCACCCAGGTGCTCGTGAGAAATGGTGAAACCGTTGTGCTGGGCGGTATCTTCCAGTCTGAAGTCTCA
ACCACGGTCACCAAGACGCCTTTTCTCGGGGACATTCCTTACCTTGGGCGCCTTTTCAAGCGGACAGAGAACGTGGATGA
GCGCAGCGAGCTGCTGGTATTCATCACTCCGAAGATTCTCAAGGGCGACCTGATTGAATAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(295-341)

Bacterial type II and III secretion system protein (Secretin)

(526-681)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(369-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

50.774

100

0.526
  comQ Acinetobacter baylyi ADP1

41.525

100

0.429
  pilQ Acinetobacter baumannii strain A118

39.477

100

0.418
  pilQ Acinetobacter baumannii D1279779

39.477

100

0.418
  pilQ Legionella pneumophila strain ERS1305867

40.296

98.397

0.396
  pilQ Legionella pneumophila strain Lp02

40.148

98.397

0.395