Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   SCRE_RS04440 Genome accession   NC_022236
Coordinates   891418..892260 (-) Length   280 a.a.
NCBI ID   WP_020997792.1    Uniprot ID   U2ZGW0
Organism   Streptococcus constellatus subsp. pharyngis C232     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 886418..897260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCRE_RS04425 (SCRE_0871) trmFO 887139..888473 (-) 1335 WP_006267737.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  SCRE_RS04430 (SCRE_0872) - 888680..889177 (-) 498 WP_006267892.1 hypothetical protein -
  SCRE_RS04435 (SCRE_0873) topA 889239..891332 (-) 2094 WP_148290513.1 type I DNA topoisomerase -
  SCRE_RS04440 (SCRE_0874) dprA/cilB/dalA 891418..892260 (-) 843 WP_020997792.1 DNA-processing protein DprA Machinery gene
  SCRE_RS04445 (SCRE_0875) - 892345..892902 (-) 558 WP_006267839.1 sugar O-acetyltransferase -
  SCRE_RS09980 - 892968..893181 (-) 214 Protein_873 CAT RNA binding domain-containing protein -
  SCRE_RS04450 (SCRE_0876) - 893178..893960 (-) 783 WP_006267744.1 ribonuclease HII -
  SCRE_RS04455 (SCRE_0877) ylqF 893947..894798 (-) 852 WP_006267825.1 ribosome biogenesis GTPase YlqF -
  SCRE_RS04460 (SCRE_0878) - 894917..895834 (-) 918 WP_003069057.1 MBL fold metallo-hydrolase -
  SCRE_RS04465 (SCRE_0879) - 895838..896950 (-) 1113 Protein_877 acyl-CoA dehydrogenase family protein -

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 31165.75 Da        Isoelectric Point: 5.4273

>NTDB_id=61464 SCRE_RS04440 WP_020997792.1 891418..892260(-) (dprA/cilB/dalA) [Streptococcus constellatus subsp. pharyngis C232]
MDNFDIYKLKTAGLTNQQVINVLEYAEIREKELSVKDMAVVSECRNPALFIEKYLQLDDDLLRQEFEKFPSFSILEDVYP
WDLSEIYNPPVLLFYKGNLDLLKLPKVAVVGSRNSSRVGSQSVQKIIKELNNELVIVSGLARGIDTSAHMAALQNGGRTI
AVIGTGLDVFYPRANKKLQSYLGEHHLVLSEYGSEAEPLKFHFPERNRIIAGLCRGVIVAEAKMRSGSLITCERAMEEGR
DVFAIPGSILDGRSDGCHHLIQEGAKCIASGADVLSEFQF

Nucleotide


Download         Length: 843 bp        

>NTDB_id=61464 SCRE_RS04440 WP_020997792.1 891418..892260(-) (dprA/cilB/dalA) [Streptococcus constellatus subsp. pharyngis C232]
ATGGATAATTTTGATATTTATAAACTAAAAACAGCTGGTTTGACCAACCAACAAGTCATCAATGTATTGGAATACGCAGA
AATTCGCGAAAAAGAATTGTCAGTCAAAGATATGGCAGTTGTCTCCGAATGTCGTAATCCTGCTCTTTTTATTGAAAAAT
ATTTACAATTAGATGATGATTTATTACGTCAAGAATTTGAGAAATTCCCATCTTTTTCTATCTTGGAAGATGTATATCCT
TGGGATTTGAGTGAAATTTATAATCCACCCGTTCTTCTTTTTTATAAAGGTAATTTGGATTTGCTAAAATTACCTAAAGT
AGCTGTTGTAGGAAGTCGGAATAGTAGCCGAGTAGGCAGTCAATCCGTTCAAAAGATTATTAAAGAGCTAAACAATGAAT
TGGTTATCGTGAGTGGTTTAGCACGCGGAATTGATACCTCAGCTCACATGGCAGCATTGCAAAATGGTGGTCGAACAATT
GCAGTTATCGGAACGGGTCTAGACGTTTTTTATCCAAGGGCAAATAAAAAACTCCAATCGTATTTGGGAGAACATCATCT
AGTACTTAGTGAATATGGTTCTGAAGCAGAGCCATTAAAATTTCATTTTCCAGAGCGTAATCGGATTATAGCAGGATTGT
GCAGAGGGGTTATTGTTGCTGAAGCAAAAATGCGCTCAGGCAGCCTTATTACTTGTGAGCGAGCAATGGAAGAAGGGCGA
GATGTATTTGCTATTCCAGGGTCAATTTTAGATGGAAGAAGTGATGGCTGTCATCATTTGATTCAGGAAGGAGCAAAATG
CATCGCATCAGGGGCTGACGTACTTTCTGAATTCCAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(74-269)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-60)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U2ZGW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

76.071

100

0.761
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

75.714

100

0.757
  dprA/cilB/dalA Streptococcus pneumoniae D39

75.714

100

0.757
  dprA/cilB/dalA Streptococcus pneumoniae R6

75.714

100

0.757
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

75.714

100

0.757
  dprA/cilB/dalA Streptococcus mitis SK321

75.357

100

0.754
  dprA Streptococcus mutans UA159

65

100

0.65
  dprA Lactococcus lactis subsp. cremoris KW2

56.786

100

0.568
  dprA Legionella pneumophila strain ERS1305867

43.154

86.071

0.371
  dprA Staphylococcus aureus N315

36.879

100

0.371

Multiple sequence alignment