Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NPO58_RS15185 Genome accession   NZ_CP101741
Coordinates   3074255..3075640 (-) Length   461 a.a.
NCBI ID   WP_115205727.1    Uniprot ID   -
Organism   Escherichia coli strain FY26     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3069255..3080640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPO58_RS15150 (NPO58_15115) mutT 3069704..3070102 (+) 399 WP_000736044.1 8-oxo-dGTP diphosphatase MutT -
  NPO58_RS15155 (NPO58_15120) yacG 3070190..3070387 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  NPO58_RS15160 (NPO58_15125) zapD 3070397..3071140 (-) 744 WP_001194731.1 cell division protein ZapD -
  NPO58_RS15165 (NPO58_15130) coaE 3071140..3071760 (-) 621 WP_001269535.1 dephospho-CoA kinase -
  NPO58_RS15170 (NPO58_15135) - 3071785..3071829 (+) 45 WP_120795372.1 protein YacM -
  NPO58_RS15175 (NPO58_15140) guaC 3071985..3073028 (+) 1044 WP_001217338.1 GMP reductase -
  NPO58_RS15180 (NPO58_15145) hofC 3073063..3074265 (-) 1203 WP_000157254.1 protein transport protein HofC -
  NPO58_RS15185 (NPO58_15150) pilB 3074255..3075640 (-) 1386 WP_115205727.1 type II secretion system protein GspE Machinery gene
  NPO58_RS15190 (NPO58_15155) pilA 3075650..3076090 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  NPO58_RS15195 (NPO58_15160) nadC 3076294..3077187 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NPO58_RS15200 (NPO58_15165) ampD 3077275..3077826 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NPO58_RS15205 (NPO58_15170) ampE 3077823..3078677 (+) 855 WP_000171983.1 beta-lactamase regulator AmpE -
  NPO58_RS15210 (NPO58_15175) aroP 3078720..3080090 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50730.44 Da        Isoelectric Point: 6.7135

>NTDB_id=611747 NPO58_RS15185 WP_115205727.1 3074255..3075640(-) (pilB) [Escherichia coli strain FY26]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPANNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQRLVLVTGPTGSGKTVTLYSALQMLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=611747 NPO58_RS15185 WP_115205727.1 3074255..3075640(-) (pilB) [Escherichia coli strain FY26]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACACGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTAGAACAACGCGCGTCTGATATTCATATCGAACCAGCGAACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGAGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAATGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAACTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTAGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTAGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

40.821

100

0.41
  pilB Legionella pneumophila strain ERS1305867

49.219

83.297

0.41
  pilB Acinetobacter baylyi ADP1

39.316

100

0.399
  pilB Vibrio campbellii strain DS40M4

47.668

83.731

0.399
  pilB Vibrio cholerae strain A1552

45.897

84.599

0.388
  pilB Vibrio parahaemolyticus RIMD 2210633

45.385

84.599

0.384
  pilB Haemophilus influenzae 86-028NP

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

43.896

83.514

0.367
  pilB Haemophilus influenzae Rd KW20

43.669

83.948

0.367
  pilB Acinetobacter baumannii D1279779

42.64

85.466

0.364