Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NPO58_RS15190 Genome accession   NZ_CP101741
Coordinates   3075650..3076090 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain FY26     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3070650..3081090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPO58_RS15165 (NPO58_15130) coaE 3071140..3071760 (-) 621 WP_001269535.1 dephospho-CoA kinase -
  NPO58_RS15170 (NPO58_15135) - 3071785..3071829 (+) 45 WP_120795372.1 protein YacM -
  NPO58_RS15175 (NPO58_15140) guaC 3071985..3073028 (+) 1044 WP_001217338.1 GMP reductase -
  NPO58_RS15180 (NPO58_15145) hofC 3073063..3074265 (-) 1203 WP_000157254.1 protein transport protein HofC -
  NPO58_RS15185 (NPO58_15150) pilB 3074255..3075640 (-) 1386 WP_115205727.1 type II secretion system protein GspE Machinery gene
  NPO58_RS15190 (NPO58_15155) pilA 3075650..3076090 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  NPO58_RS15195 (NPO58_15160) nadC 3076294..3077187 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NPO58_RS15200 (NPO58_15165) ampD 3077275..3077826 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NPO58_RS15205 (NPO58_15170) ampE 3077823..3078677 (+) 855 WP_000171983.1 beta-lactamase regulator AmpE -
  NPO58_RS15210 (NPO58_15175) aroP 3078720..3080090 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=611748 NPO58_RS15190 WP_000360914.1 3075650..3076090(-) (pilA) [Escherichia coli strain FY26]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=611748 NPO58_RS15190 WP_000360914.1 3075650..3076090(-) (pilA) [Escherichia coli strain FY26]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AATTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363